Urine Metabolites and Bioactive Compounds from Functional Food: Applications of Liquid Chromatography Mass Spectrometry

Ong, Eng Shi

doi:10.1080/10408347.2023.2235442

Cited by 4 publications

(2 citation statements)

References 87 publications

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“…Specifically, LC/MSMS was performed to identify the presence of α-Mangostin, γ-Mangostin and Gartanin from MPE with reference to the MSMS spectra obtained from the reference standards (Table 1, Supplementary Figure S1), which is also consistent with a recent report [40]. Subsequently, concentrations of the target compounds were determined at the various applied temperatures based on external calibration via reverse calculation using the peak areas and standard curves produced during the LC/UV methodology, as previously mentioned [41]. Based on target compounds and peaks detected using LC/UV, PHWE extraction at different temperatures yielded MPEs with unique chemical profiles (Figure 1A).…”

Section: Phwe and Chemical Standardization Of Mpementioning

confidence: 53%

Pressurized Hot Water Extraction of Mangosteen Pericarp and Its Associated Molecular Signatures in Endothelial Cells

Tan,

Shanmugham,

Chin

et al. 2023

Antioxidants

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The mangosteen (Garcinia mangostana L.) pericarp is known to be rich in potent bioactive phytochemical compounds such as xanthones, which possess pharmacologically important antioxidant activity and beneficial cardiometabolic properties. Mangosteen pericarp is typically classified as unavoidable food waste and discarded, despite being rich in bioactive phytochemical compounds that therefore present an exciting opportunity for valorization. Thus, this study aims to extract phytochemical compounds from mangosteen pericarp using pressurized hot water extraction (PHWE) and determine its biological effects in endothelial cells using RNA sequencing. Liquid chromatography with MS/MS (LC/MSMS) and UV detection (LC/UV) was subsequently used to identify three key phytochemical compounds extracted from the mangosteen pericarp: α-Mangostin, γ-Mangostin, and Gartanin. Within the tested range of extraction temperatures by PHWE, our results demonstrated that an extraction temperature of 120 °C yielded the highest concentrations of α-Mangostin, γ-Mangostin, and Gartanin with a concomitant improvement in antioxidant capacity compared to other extraction temperatures. Using global transcriptomic profiling and bioinformatic analysis, the treatment of endothelial cells with mangosteen pericarp extracts (120 °C PHWE) for 48 h caused 408 genes to be differentially expressed. Furthermore, our results demonstrated that key biological processes related to “steroid biosynthesis and metabolism”, likely involving the activation of the AMPK signaling pathway, were upregulated by mangosteen pericarp extract treatment. In conclusion, our study suggests a green extraction method to valorize phytochemical compounds from mangosteen pericarp as a natural product with potential beneficial effects on cardiometabolic health.

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Section: Phwe and Chemical Standardization Of Mpementioning

confidence: 53%

Pressurized Hot Water Extraction of Mangosteen Pericarp and Its Associated Molecular Signatures in Endothelial Cells

Tan,

Shanmugham,

Chin

et al. 2023

Antioxidants

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“…Metabolomic methods provide a deeper understanding of the metabolites that influence physiological responses to broccoli consumption. − However, nontargeted metabolomic analyses, while being efficient in discovering numerous metabolites, may be prone to interference and require expensive detection equipment [e.g., LC–tandem mass spectrometry (MS/MS) and HRMS]. , Highly selective analytical methods are more suitable for metabolites with similar chemical structures such as indole-active compounds. , Urine, owing to its complexity and noninvasive sampling, is considered an ideal biomaterial for measuring metabolites. − However, the low content of indole metabolites in urine necessitates selective sample preparation and accurate detection. Molecularly imprinted polymers (MIPs), which are known for their chemical stability and selectivity, offer a potential solution; however, the development of MIPs with ideal selectivity in aqueous solutions remains challenging. − …”

Section: Introductionmentioning

confidence: 99%

Development and Application of a Selective Analytical Method for Indole Metabolites in Urine: Dietary Exposure Biomarkers for Broccoli Consumption

Yuan,

Wang,

Zhang

et al. 2024

J. Agric. Food Chem.

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The identification of dietary exposure biomarkers is crucial for advancing our understanding of the health benefits of specific foods. Broccoli, a vegetable with well-known anticancer properties, contains active ingredients, such as isothiocyanates with indole side chains. Hence, indole metabolites related to broccoli consumption have the potential to serve as biomarkers of dietary exposure. In this work, we developed a new analytical method for indole metabolites in urine using a poly(deep eutectic solvents)molecularly imprinted polymer/vinyl-functionalized graphene oxide (PDESs-MIP/VGO) in miniaturized centrifugal pipet-tip solidphase extraction (CPT-SPE) coupled with liquid chromatography. This method integrates the strengths of PDESs-MIP/VGO, including rich adsorption interactions, high adsorption capacity, and excellent selectivity, with the simplicity and cost-effectiveness of CPT-SPE. The proposed method demonstrated low limits of quantification (1.2−2.5 ng mL −1 ), high accuracy (91.7−104.8%), and good precision (relative standard deviation ≤4.4%). By applying this method to analyze indole metabolites in urine, our results suggested that indole-3-carbinol and indole-3-acetonitrile have the potential to emerge as reliable dietary exposure biomarkers for broccoli intake. Furthermore, highly selective analytical methods based on molecular imprinting technology are advantageous for precise screening and analysis of dietary exposure biomarkers associated with food consumption.

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Urine biomarkers can outperform serum biomarkers in certain diseases

Xue,

Yang,

et al. 2023

URINE

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Urine Metabolites and Bioactive Compounds from Functional Food: Applications of Liquid Chromatography Mass Spectrometry

Cited by 4 publications

References 87 publications

Pressurized Hot Water Extraction of Mangosteen Pericarp and Its Associated Molecular Signatures in Endothelial Cells

Pressurized Hot Water Extraction of Mangosteen Pericarp and Its Associated Molecular Signatures in Endothelial Cells

Development and Application of a Selective Analytical Method for Indole Metabolites in Urine: Dietary Exposure Biomarkers for Broccoli Consumption

Urine biomarkers can outperform serum biomarkers in certain diseases

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