Urine Hydrogen Peroxide Levels and Their Relation to Outcome in Patients with Sepsis, Septic Shock, and Major Burn Injury

Abstract

Hydrogen peroxide (H2O2) and oxidative stress have been suggested as possible instigators of both the systemic inflammatory response and the increased vascular permeability associated with sepsis and septic shock. We measured H2O2 concentrations in the urine of 82 patients with severe infections, such as sepsis, septic shock, and infections not fulfilling sepsis-3 criteria, in patients with major burn injury with associated systemic inflammation, and healthy subjects. The mean concentrations of H2O2 were found to be lower in patients with severe infections compared to burn injury patients and healthy subjects. Patients with acute kidney injury (AKI), vs. those without AKI, in all diagnostic groups displayed higher concentrations of urine H2O2 (p < 0.001). Likewise, urine concentrations of H2O2 were higher in non-survivors as compared to survivors (p < 0.001) at day 28 in all diagnostic groups, as well as in patients with severe infections and burn injury (p < 0.001 for both). In this cohort, increased H2O2 in urine is thus associated with mortality in patients with sepsis and septic shock as well as in patients with burn injury.

A novel enzyme-free long-lasting chemiluminescence system based on a luminol functionalized β-cyclodextrin hydrogel for sensitive detection of H2O2 in urine and cells

“…The concentration of H 2 O 2 in normal urine is 6.7–14.6 μmol L −1 , which indicates that the developed CL sensor can meet the detection requirements of real samples. 43 In this work, the H 2 O 2 in human urine samples were determined under alkaline conditions. The human urine samples were provided by three healthy adult volunteers.…”

Rolling Circle Amplification/G‐Quadruplex‐Based Dual‐Signal Ratiometric Electrochemical Aptasensor for Ultrasensitive Detection of Pathogenic Bacteria

“…Clinical medicine has placed urgent demands for bacteriological testing to help deal with this infection type. [1,2] At present, clinical bacterial detection methods mainly include traditional biochemical identification, immunological detection methods, molecular biological analysis, mass spectrometry, and others. [3][4][5] However, these methods carry the disadvantages of being time consuming, operationally complex, and consuming patient samples, which limit their applicability in certain specific clinical scenarios.…”

“…Bacterial infections are acute systemic infections caused by pathogenic bacteria, or opportunistic pathogens entering the blood circulation and producing toxins and other metabolites. Clinical medicine has placed urgent demands for bacteriological testing to help deal with this infection type [1,2] . At present, clinical bacterial detection methods mainly include traditional biochemical identification, immunological detection methods, molecular biological analysis, mass spectrometry, and others [3–5] .…”

Kidney Transplant Recipients Show Limited Lung Diffusion Capacity but Similar Hydrogen Peroxide Exhalation as Healthy Matched Volunteers: A Pilot Study

“…Hydrogen peroxide (H 2 O 2 ) is one of the most over-produced ROS in CKD patients [1,2]. Increased concentrations of H 2 O 2 were reported in the urine, plasma, and exhaled breath condensate (EBC) of the analyzed CKD patients [10][11][12][13][14][15]. EBC is a composition of (A)particles or droplets that are aerosolized from the airway lining fluid, (B)-distilled water that arises through condensation of water vapor from the airway surface, and (C)-water soluble volatiles that are exhaled and absorbed into the condensing breath [16].…”