IntroductIonThere are several factors that could potentially alter the normal man steroid profile for example an age, sex and diet. From doping control view-point, for the adequate interpretation of the analytical results it is necessary to possess an ability of selecting adaptive changes in the body that response to physiological impulses having nothing in common with intended doping. Among the best recognized factors influencing quantitatively the androgenic profile are training loads forcing high physical activity, restricted energy intake and training specificity of a given sport discipline and interethnic variation [3,4,10,16].Although numerous studies were conducted to evaluate the effect of various stimuli, including pre-competition stress in competing athletes, on the behavior of endogenous blood androgens in humans, the effect of stress on urinary androgen excretion is still pure recognized. It should be noticed that under stress condition or following an exertion, the level of an androgen in urine does not always closely reflects that in blood because of strong effect of mentioned stimuli on renal function. For that reason the behavior proof urinary T/Et ratio being a proof of testosterone abuse by athletes may fluctuate AbstrAct: In the second part of twentieth century anabolic-androgenic steroids were introduced into doping practice and received continuously increasing significance. In order to prove the usage of doping substances, the determination of steroid profile in the urine came into practice. Several factors may be responsible for alterations in the normal steroid profile for example age, sex and diet. The aim of this study was to find out, whether the psychological stress may cause modifications in the steroid profile and T/Et ratio. The effect of physical activity was also considered. The steroid profile was determined in the group of 34 students being in non-stress conditions and under stress immediately before an important university exam. The intensity of stress was rated by self-reported questionnaire. The GC/MS method was applied to determine the steroid profile in the urine samples. The results of the experiment have shown that psychological stress may cause significant changes in the steroid profile, especially in females. Physical activity, independently of stress significantly modified the steroid profile. In summary, observed changes in steroid profile suggest, that major fluctuations of T/Et and A/E ratios under the influence of stressogenic factors and physical activity are unlikely.KEY wordS: doping, steroid profile, stress, physical activity dependently on external conditions. This problem is an important one, when anti-doping controls are carried out among competitors