Urinary Tract Infection

Byron, Julie K.

doi:10.1016/j.cvsm.2018.11.005

Cited by 79 publications

(94 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is unsurprising because urinary tract infection (UTI) is a common diagnosis in companion animals (24). Moreover, UTIs in cats and dogs usually involve a single agent: E. coli (25).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Extended-Spectrum β-Lactamase–Producing Escherichia coli From Dogs and Cats Admitted to a Veterinary Teaching Hospital in Taipei, Taiwan From 2014 to 2017

2020

View full text Add to dashboard Cite

Extended-spectrum β-lactamases (ESBLs) are enzymes that mediate resistance to newer β-lactam antibiotics, including extended-spectrum cephalosporins and monobactams. The production of ESBL is primarily plasmid mediated, and such plasmids often comprise the genes that encode resistance to other classes of antimicrobials, such as aminoglycosides and fluoroquinolones. Therefore, ESBL-producing microorganisms leave clinicians with limited therapeutic options in both human and veterinary medicine. Compared with human medicine, information regarding ESBL-producing microorganisms is limited in veterinary medicine. We screened for ESBL-producing Escherichia coli in dogs and cats admitted to National Taiwan University Veterinary Hospital, Taipei, from 2014 to 2017 and further analyzed the genotypes and phylogenetic traits of these ESBL producers. Double disk tests specified by the Clinical and Laboratory Standards Institute were performed on 283 E. coli isolates and revealed a total of 65 E. coli (54 from dogs and 11 from cats) with the ESBL phenotype (22.8%). bla CTX−M−1 group and bla CTX−M−2group were the most commonly identified ESBL gene groups. bla CTX−M−55 was the main ESBL gene within the bla CTX−M−1group , whereas the bla CTX−M−2group contained only bla CTX−M−124. The ESBL-producing E. coli were all resistant to ampicillin. The resistance rate to ceftiofur, doxycycline, enrofloxacin, and ciprofloxacin was 93.8, 73.8, 80, and 78.5%, respectively. Of the antibiotics tested, greater sensitivity to imipenem and gentamicin was noted. Multilocus sequence typing indicated that ST457, ST131, and ST648 were the most common sequence types. Our study identified eight ST131/O25b isolates, which is a global zoonotic clone of public health concern. The major ESBL genes Huang et al. ESBL-Producing Escherichia coli of these clones were bla CTX−M−174 and bla CTX−M−194. Because companion animals such as dogs and cats are in close contact with humans, the characterization of ESBL producers originating from them is crucial from the perspective of both public health and veterinary medicine.

show abstract

“…This is unsurprising because urinary tract infection (UTI) is a common diagnosis in companion animals (24). Moreover, UTIs in cats and dogs usually involve a single agent: E. coli (25).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Extended-Spectrum β-Lactamase–Producing Escherichia coli From Dogs and Cats Admitted to a Veterinary Teaching Hospital in Taipei, Taiwan From 2014 to 2017

2020

View full text Add to dashboard Cite

show abstract

“…Urinary tract disease is one of the most common diagnoses in veterinary medicine (Byron, 2019; Ling, 1984). In addition, dogs are a valuable translational model for many human diseases, including urogenital diseases like bladder cancer (Knapp et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Evaluating Extraction Methods to Study Canine Urine Microbiota

Mrofchak¹,

Madden²,

Evans³

et al. 2021

Preprint

View full text Add to dashboard Cite

The urinary microbiota is the collection of microbes present in urine that play a role in host health. Studies of urine microbiota have traditionally relied upon culturing methods aimed at identifying pathogens. However, recent culture-free sequencing studies of the urine microbiota have determined that a diverse array of microbes are present in health and disease. To study these microbes and their potential role in diseases like bladder cancer or interstitial cystitis, consistent extraction and detection of microbial DNA from urine is critical. However, urine is a low biomass substrate, requiring sensitive methods to capture DNA and making the risk of contamination high. To address this challenge, we collected urine samples from ten healthy dogs and extracted DNA from each sample using five different commercially available extraction methods. Extraction methods were compared based on total and bacterial DNA concentrations and microbial community composition and diversity assessed through 16S rRNA gene sequencing. Significant differences in the urinary microbiota were observed by dog and sex but not extraction method. The Bacteremia kit yielded the highest total DNA concentrations (Kruskal-Wallis, p = 0.165, not significant) and the highest bacterial DNA concentrations (Kruskal-Wallis, p = 0.044). Bacteremia also extracted bacterial DNA from the greatest number of samples. Taken together, these results suggest that the Bacteremia kit is an effective option for studying the urine microbiota. This work lays the foundation to study the urine microbiome in a wide range of urogenital diseases in dogs and other species.HighlightsCanine urine microbiota differed by sex and dog but not extraction method.Qiagen Bacteremia kit yielded the highest bacterial DNA concentrations from urine.The Bacteremia kit extracted bacterial DNA from the greatest number of samples.Absolute abundance of Sphingomonas species increased in female dog urine.Pasteurellaceae bacterium canine oral taxon 272 increased in male dog urine.

show abstract

“…2019). As described in this manuscript, dogs were prescribed nitrofurantoin for the treatment of recurrent lower UTIs with 12 of 14 dogs diagnosed with an underlying comorbidity likely contributing to their recurrent infections ( i.e ., urinary tract abnormalities, chronic immunosuppression, chronic myelopathy or newly diagnosed or poorly controlled endocrinopathy) (Byron 2019). All dogs had demonstrated bacteriuria via urine culture due to common uropathogens associated with UTIs.…”

Section: Discussionmentioning

confidence: 99%

Administration of nitrofurantoin in dogs with lower urinary tract infections: 14 cases (2013‐2019)

Leuin¹,

Hartmann

Viviano

2020

J of Small Animal Practice

View full text Add to dashboard Cite

The purpose of this study was to describe the clinical use of nitrofurantoin in client-owned dogs with lower urinary tract infections. The primary aim was to describe the patient population, dosage, treatment duration, outcome and side effects. Materials and MethOds: Medical records in an institution were retrospectively reviewed from July 2013 to January 2019. results: Nitrofurantoin was prescribed in this clinical population of 14 client-owned dogs for lower urinary tract infections. Recurrent urinary tract infection was the clinical diagnosis in all dogs. Each dog's urinary tract infection was associated with a nitrofurantoin-susceptible, multidrug-resistant uropathogen. The median dosage and duration of nitrofurantoin treatment was 4.3 mg/kg by mouth every 8 hours for 14 days. Twelve of the 14 dogs had successful outcomes including bacteriologic cure (n = 9), clinical cure (2) and resolution of target bacteria (1). Treatment failures (n = 2) were associated with uropathogens developing progressive nitrofurantoin resistance. clinical significance: In some dogs with recurrent lower urinary tract infections, nitrofurantoin may be an effective antibiotic for treatment of nitrofurantoin-susceptible uropathogens. Treatment failures were associated with progressive uropathogen resistance. Urine bacterial culture and quantitative susceptibility testing are essential to initiating and monitoring treatment due to the multidrug-resistant isolates and, in some cases, persistent bacteriuria in the face of treatment.

show abstract

Urinary Tract Infection

Cited by 79 publications

References 32 publications

Characteristics of Extended-Spectrum β-Lactamase–Producing Escherichia coli From Dogs and Cats Admitted to a Veterinary Teaching Hospital in Taipei, Taiwan From 2014 to 2017

Characteristics of Extended-Spectrum β-Lactamase–Producing Escherichia coli From Dogs and Cats Admitted to a Veterinary Teaching Hospital in Taipei, Taiwan From 2014 to 2017

Evaluating Extraction Methods to Study Canine Urine Microbiota

Administration of nitrofurantoin in dogs with lower urinary tract infections: 14 cases (2013‐2019)

Contact Info

Product

Resources

About