Urinary screening for methylphenidate (Ritalin) abuse: a comparison of liquid chromatography–tandem mass spectrometry, gas chromatography–mass spectrometry, and immunoassay methods

Eichhorst, Jeff; Etter, Michele L.; Lepage, Joyce; Lehotay, Denis C.

doi:10.1016/j.clinbiochem.2003.11.006

Cited by 39 publications

(31 citation statements)

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“…Des techniques ELISA ont été mises au point pour le screening urinaire (68)(69), mais avec parfois un taux élevé de faux positifs (69). Actuellement, la majorité des auteurs s'accordent à reconnaître la supériorité de la chromatographie liqui¬ de couplée à la spectrométrie de masse (CL-SM), et surtout à la spectrométrie de masse en tandem (CL-SM-SM) (61,69,70 (63), à la chro¬ matographie en phase gazeuse couplée à un détecteur thermoionique (62,64), puis à la spectrométrie de masse (CG-SM) en ionisation chimique positive ou négative (58,65,66) (67). Des techniques ELISA ont été mises au point pour le screening urinaire (68)(69), mais avec parfois un taux élevé de faux positifs (69).…”

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“…Actuellement, la majorité des auteurs s'accordent à reconnaître la supériorité de la chromatographie liqui¬ de couplée à la spectrométrie de masse (CL-SM), et surtout à la spectrométrie de masse en tandem (CL-SM-SM) (61,69,70 (63), à la chro¬ matographie en phase gazeuse couplée à un détecteur thermoionique (62,64), puis à la spectrométrie de masse (CG-SM) en ionisation chimique positive ou négative (58,65,66) (67). Des techniques ELISA ont été mises au point pour le screening urinaire (68)(69), mais avec parfois un taux élevé de faux positifs (69). Actuellement, la majorité des auteurs s'accordent à reconnaître la supériorité de la chromatographie liqui¬ de couplée à la spectrométrie de masse (CL-SM), et surtout à la spectrométrie de masse en tandem (CL-SM-SM) (61,69,70 …”

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Prescription de chlorhydrate de méthylphénidate : la vigilance s'impose

Pélissier-Alicot¹

2006

Ann Toxicol Anal

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Prescription de chlorhydrate de méthylphénidate : la vigilance s'impose

Pélissier-Alicot¹

2006

Ann Toxicol Anal

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“…Several analytical methods have been developed for detecting MPH (Ritalin) or AP (Adderall) in biological samples. They include the enzyme-linked immunosorbent assay (ELISA), 17 gas chromatography-mass spectrometry (GC-MS), [17][18][19] and liquid chromatography-tandem mass spectrometry (LC-MS/ *Reprint requests to Jin Young Kim E-mail: paxus@spo.go.kr…”

Section: Introductionmentioning

confidence: 99%

“…For any reuse, redistribution, or reproduction of a work, users must clarify the license terms under which the work was produced. 17,[20][21][22] but none of these studies included the confirmation method for simultaneous determination of MPH and AP in urine. The analysis of AP and HAP in urine has been generally carried out by GC-MS methods, 18,19 whereas LC-MS/MS has been proposed for the analysis of MPH and RA.…”

Section: Introductionmentioning

confidence: 99%

“…The analysis of AP and HAP in urine has been generally carried out by GC-MS methods, 18,19 whereas LC-MS/MS has been proposed for the analysis of MPH and RA. 17,[20][21][22] The major advantages of LC-MS/MS technique over GC-MS are its applicability to broad range of compounds, high specificity, direct detection of hydrophilic analytes without derivatization and a high degree of verification by using multiple reaction monitoring (MRM) transitions. [23][24][25] Due to these advantages, we used LC-MS/ MS method for simultaneous analysis of MPH, RA, AP and HAP in urine.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of Methylphenidate, Amphetamine and their Metabolites in Urine using Direct Injection Liquid Chromatography-Tandem Mass Spectrometry

Kwon¹,

Suh²,

In³

et al. 2014

Mass Spectrometry Letters

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Nonmedical use of prescription stimulants such as methylphenidate (MPH) and amphetamine (AP) by normal persons has been increased to improve cognitive functions. Due to high potential for their abuse, reliable analytical methods were required to detect these prescription stimulants in biological samples. A direct injection liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and implemented for simultaneous determination of MPH, AP and their metabolites ritalinic acid (RA) and 4-hydroxyamphetamine (HAP) in human urine. Urine sample was centrifuged and the upper layer (100 µL) was mixed with 800 µL of distilled water and 100 µL of internal standards (0.2 µg/mL in methanol). The mixture was then directly injected into the LC-MS/MS system. The mobile phase was composed of 0.2% formic acid in distilled water (A) and acetonitrile (B). Chromatographic separation was performed by using a Capcell Pak MG-II C18 (150 mm × 2.0 mm i.d., 5 µm, Shiseido) column and all analytes were eluted within 5 min. Linear least-squares regression with a 1/x weighting factor was used to generate a calibration curve and the assay was linear from 20 to 1500 ng/mL (HAP), 40-3000 ng/mL (AP and RA) and 2-150 ng/mL (MPH). The intra-and inter-day precisions were within 16.4%. The intra-and inter-day accuracies ranged from -15.6% to 10.8%. The limits of detection for all the analytes were less than 4.7 ng/mL. The suitability of the method was examined by analyzing urine samples from drug abusers.

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Methylphenidate

2012

Medical Toxicology of Drug Abuse

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Urinary screening for methylphenidate (Ritalin) abuse: a comparison of liquid chromatography–tandem mass spectrometry, gas chromatography–mass spectrometry, and immunoassay methods

Cited by 39 publications

References 4 publications

Prescription de chlorhydrate de méthylphénidate : la vigilance s'impose

Prescription de chlorhydrate de méthylphénidate : la vigilance s'impose

Simultaneous Determination of Methylphenidate, Amphetamine and their Metabolites in Urine using Direct Injection Liquid Chromatography-Tandem Mass Spectrometry

Methylphenidate

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