Simultaneous Determination of Methylphenidate, Amphetamine and their Metabolites in Urine using Direct Injection Liquid Chromatography-Tandem Mass Spectrometry

Kwon, Woonyong; Suh, SungIll; In, Moon Kyo; Kim, Jin Young

doi:10.5478/msl.2014.5.4.104

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…A body of work has been done on how to identify the presence of these drugs (25B-NBF, 25C-NBF, and DMBMPP) in the human body for drug detection purposes [37][38][39][40][41] , however little is known about their overall effects and mechanisms of action including potential effects on the microtubule cytoskeleton. As such, there is very limited dosage research for 25B-NBF, 25C-NBF, and DMBMPP.…”

Section: Discussionmentioning

confidence: 99%

A molecular analysis of substituted phenylethylamines as potential microtubule targeting agents through in-vitro microtubule-polymerization activity

Abreu

Barkdull

Munoz

et al. 2023

Preprint

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Natural phenethylamines are trace amine neurotransmitters associated with dopamine transmission and related illnesses such Parkinson’s disease, and addiction. Synthetic phenethylamines can have psychoactive and hallucinogenic effects due to their high affinity with the 5-HT2A receptor. Evidence indicates phenethylamines can directly alter the microtubule cytoskeleton being structurally similar to the microtubule destabilizing agent colchicine, however little work has been done on this interaction. As microtubules provide neuron structure, intracellular transport, and influence synaptic plasticity the interaction of phenethylamines with microtubules is important for understanding the potential harms, or potential pharmaceutical use of phenethylamines. We investigated 110 phenethylamines and their interaction with microtubules. Here we performed molecular docking of these compounds at the colchicine binding site and ranked them via binding energy. The top 10% of phenethylamines were further screened based on pharmacokinetic and physicochemical properties derived from SwissADME and LightBBB. Based on these properties 25B-NBF, 25C-NBF, and DMBMPP were tested in in-vitro microtubule polymerization assays showing that they alter microtubule polymerization dynamics in a dose dependent manner. As these compounds can rapidly cross the blood brain barrier and directly affect cytoskeletal dynamics, they have the potential to modulate cytoskeletal based neural plasticity. Further investigations into these mechanisms are warranted.

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Section: Discussionmentioning

confidence: 99%

A molecular analysis of substituted phenylethylamines as potential microtubule targeting agents through in-vitro microtubule-polymerization activity

Abreu

Barkdull

Munoz

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Seo et al examined the stability of 25H‐NBOMe, 25B‐NBOMe, 25 E‐NBOMe, 25‐NBOMe, 25C‐NBOH, 25I‐NBOH, 25B‐NBF, 25C‐NBF, and 25I‐N (Seo et al, 2019). They found that all analytes were stable after three freeze/thaw cycles, when stored at room temperature for 1 day, and when stored at 4°C post‐preparation.…”

Section: Stability Of Forensically Relevant Drugs In Biological Matricesmentioning

confidence: 99%

Drug stability in forensic toxicology

Nisbet

DiEmma

Scott

2023

WIREs Forensic Science

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Knowledge of the stability of analytes in solvents and biological matrices is of high importance in the field of forensic toxicology. This is particularly true where quantitative analysis is undertaken; degradation of analytes will result in under-estimation of concentrations, whereas the production of analytes/ metabolites will lead to over-estimation. Although stability is included as part of method validation, this typically focuses on processed sample stability, and the impact of freeze/thaw cycles upon analytes. Although beneficial for method evaluation, this does not assist laboratory analysts with the short-and long-term storage of samples prior to this step and does not consider the impact the biological matrix may have on the degradation or production of the analyte in question. The timeframe for these studies is also relatively short (typically 72 h), which does not cover the time frame between sample receivership and analysis for many forensic cases. This review collects previously published work on long-term stability studies, grouping compounds into their associated drug classes and matrices. Research shows that the majority of compounds are more stable at lower storage temperatures, and that analysis should be completed as quickly as possible. It is advised that analyte stability be considered prior to any interpretation of concentrations in a forensic setting.

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“…The analyte and IS were detected using MRM mode with m/z 56.1, 84.2 and 234.1 for the analyte and 183.1 and 260.2 for the IS, respectively [39]. Other LC-MS/MS methods for quantification of MPH in human plasma [135][136][137], blood, oral fluids [137], hair [138], and urine samples [139,140] are reported in Table 2.…”

Section: Liquid Chromatography (Lc)mentioning

confidence: 99%

Review on analytical methods for quantification of ADHD drugs in human biological samples

Sundari¹,

Amuthalakshmi²,

Nalini³

2020

Reviews in Analytical Chemistry

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Attention deficit hyperactivity disorder (ADHD) is a common neuro-developmental disorder. The symptoms of ADHD include difficulty in attention, memory and impulse control. Many pharmaceutical formulations (stimulants and non-stimulants) are available on the market to treat ADHD symptoms. The most commonly used drugs for treatment are amphetamine, methylphenidate, atomoxetine, bupropion, guanfacine and clonidine. In the field of pharmaceuticals, bioanalysis is an important tool used for the quantification of drugs and their metabolites present in biological samples using various analytical methods. Although a number of analytical methods were reported for the quantification of these drugs in biological samples of experimental animals, due to species differences, it is important to develop analytical methods to quantify these drugs in human biological samples to aid forensic and pharmacokinetic studies. In this review, we compile the bio-analytical methods such as spectrophotometry, spectrofluorimetry, mass spectrometry, electrophoresis, liquid chromatography and gas chromatography used for the quantification of ADHD drugs in human biological samples such as blood, plasma, serum, oral fluids, sweat, hair and urine based on earlier published articles from various journals.

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Simultaneous Determination of Methylphenidate, Amphetamine and their Metabolites in Urine using Direct Injection Liquid Chromatography-Tandem Mass Spectrometry

Cited by 4 publications

References 23 publications

A molecular analysis of substituted phenylethylamines as potential microtubule targeting agents through in-vitro microtubule-polymerization activity

A molecular analysis of substituted phenylethylamines as potential microtubule targeting agents through in-vitro microtubule-polymerization activity

Drug stability in forensic toxicology

Review on analytical methods for quantification of ADHD drugs in human biological samples

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