Urinary Proteome Analysis to Exclude Severe Vesicoureteral Reflux

Drube, Jens; Schiffer, Eric; Lau, Esther; Petersen, Claus; Kirschstein, Martin; Kemper, Markus J.; Lichtinghagen, Ralf; Ure, Benno M.; Mischak, Harald; Pape, Lars; Ehrich, J. H. H.

doi:10.1542/peds.2010-3467

Cited by 28 publications

(19 citation statements)

References 51 publications

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“…Because of its high negative predictive value, this analysis would be useful as a screening test to detect high‐grade VUR. Only children with a positive result would then require VCUG . Because the urinary proteome can include many urinary fibrosis‐related peptides from the extracellular matrix, it can act as a non‐invasive liquid biopsy to identify patients with CKD .…”

Section: Are There Any Alternatives To Vcug?mentioning

confidence: 99%

Pediatric voiding cystourethrography: An essential examination for urologists but a terrible experience for children

et al. 2018

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Voiding cystourethrography is the most important fluoroscopic examination in pediatric urology for the investigation of lower urogenital tract diseases, such as vesicoureteral reflux or urethral stricture. However, this invasive procedure imposes a significant burden on children and their parents, and recently there has been a paradigm shift in the diagnosis and treatment of vesicoureteral reflux. In the 2011 revision, the American Academy of Pediatrics guidelines on urinary tract infection recommended abandoning routine voiding cystourethrography after the first febrile urinary tract infection. In 2014, the randomized intervention for children with vesicoureteral reflux study recommended discontinuation of routine continuous antibiotic prophylaxis for vesicoureteral reflux. The time is now ripe to radically reconsider indications for voiding cystourethrography and the procedure itself.

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Section: Are There Any Alternatives To Vcug?mentioning

confidence: 99%

Pediatric voiding cystourethrography: An essential examination for urologists but a terrible experience for children

et al. 2018

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“…45–47 The possibility that application of omics approaches to such samples could identify molecular signatures and prognostic biomarkers was suggested by findings from studies in common kidney disorders such as diabetic nephropathy, 48 allograft rejection, 49 and vesicoureteral reflux. 50 Changes in urinary miRNA profile have been detected in disorders such as lupus nephritis 51 and renal fibrosis. 52 The study of the urine metabolome by nuclear magnetic resonance spectroscopy and mass spectrometry is another emerging technology that can generate molecular fingerprints of diagnostic or prognostic value, 53 as already shown in patients with Fanconi’s syndrome.…”

Section: Opportunitiesmentioning

confidence: 99%

Rare inherited kidney diseases: challenges, opportunities, and perspectives

et al. 2014

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At least 10% of adults and nearly all children who receive renal-replacement therapy have an inherited kidney disease. These patients rarely die when their disease progresses and can remain alive for many years because of advances in organ-replacement therapy. However, these disorders substantially decrease their quality of life and have a large effect on health-care systems. Since the kidneys regulate essential homoeostatic processes, inherited kidney disorders have multisystem complications, which add to the usual challenges for rare disorders. In this review, we discuss the nature of rare inherited kidney diseases, the challenges they pose, and opportunities from technological advances, which are well suited to target the kidney. Mechanistic insights from rare disorders are relevant for common disorders such as hypertension, kidney stones, cardiovascular disease, and progression of chronic kidney disease.

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“…In a small cohort of 73 children, Drube et al. developed a CE‐MS‐based urinary biomarker panel with high sensitivity (88%) and specificity (79%) for detecting VUR, which upon validation in a larger cohort, could spare young patients from going through invasive procedures to diagnose VUR .…”

Section: Clinical and Bioanalytical Applicationsmentioning

confidence: 99%

“…High-grade vesicoureteral reflux (VUR) is a risk factor for impaired renal function and current diagnosis by voiding cystourethrography is highly invasive and only concerns a minority of children. In a small cohort of 73 children, Drube et al developed a CE-MS-based urinary biomarker panel with high sensitivity (88%) and specificity (79%) for detecting VUR, which upon validation in a larger cohort, could spare young patients from going through invasive procedures to diagnose VUR [96].…”

Section: Pediatric Nephrologymentioning

confidence: 99%

Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications

et al. 2013

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Proteome analysis using capillary electrophoresis coupled to mass spectrometry (CE-MS) has been used in a number of clinical applications in the past years. The main focus of CE-MS-based studies has been on the investigation of urine, due to the stability of the urinary proteome, ease of collection, and also the low molecular weight range of the urinary proteome, mostly peptides below 30 kDa. The reproducibility of this approach has enabled analysis of over 20 000 samples in a comparable way, giving enormous statistical power to any additional study involving this methodological setup. In this article, we review the major technological issues associated with the application of CE-MS in the routine investigation of the urinary proteome for clinical applications. We pinpoint recent developments that may have a chance to improve on the currently used approach, and highlight obstacles that need to be solved. In the second part of the article, we review the recent clinical applications, aiming to highlight relevant issues, and possible future routine applications in clinical diagnosis. In the end, we provide a short outlook, and indicate future developments to be expected, as well as problems that need to be solved to enable routine application of CE-MS in a clinical setting.

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Urinary Proteome Analysis to Exclude Severe Vesicoureteral Reflux

Cited by 28 publications

References 51 publications

Pediatric voiding cystourethrography: An essential examination for urologists but a terrible experience for children

Pediatric voiding cystourethrography: An essential examination for urologists but a terrible experience for children

Rare inherited kidney diseases: challenges, opportunities, and perspectives

Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications

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