Urinary peptidome analyses for the diagnosis of chronic kidney disease in dogs

Pelander, Lena; Brunchault, Valérie; Buffin-Meyer, Bénédicte; Klein, Julie; Breuil, Benjamin; Zürbig, Petra; Magalhães, Pedro; Mullen, William; Elliott, Jonathan; Syme, H M; Schanstra, Joost P.; Häggström, Jens; Ljungvall, Ingrid

doi:10.1016/j.tvjl.2019.05.010

Cited by 11 publications

(9 citation statements)

References 33 publications

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“…Considering all the dogs with CKD (G 1 and G 2) , F2 migrating in the zone of alpha 1globulins appears to be significantly increased in dogs with kidney disease. In agreement with the present results of this study, Pelander et al (20) and Gonzalez et al ( 25) have reported elevations of certain proteins in CKD patients with or without L. infantum infection using capillary electrophoresis coupled to mass spectrometry (CE-MS). One of these proteins is retinol binding protein (belonging to the alpha-1 globulin group of proteins), which is related to proximal tubular dysfunction (26).…”

Section: Discussionsupporting

confidence: 93%

“…Urinary capillary electrophoresis (UCE) is an available laboratory technique that can provide an easily interpretable electrophoretic pattern divided into five fractions as in serum (fraction 1 corresponding to the albumin migration zone, fraction 2 corresponding to the alpha1-globulins zone, fraction 3 corresponding to the alpha2-globulins zone, fraction 4 corresponding to beta-globulins zone, fraction 5 corresponding to the gamma globulins zone) which can be useful in the early diagnosis of the renal disease (16,20).…”

Section: Discussionmentioning

confidence: 99%

“…There are only two studies about the use of UCE in dogs. One of these studies evaluates urinary peptides excreted for the diagnosis of chronic kidney disease, while the other study establishes reference intervals for protein fractions in the urine of healthy animals in order to make a comparison with pathological processes (16,20).…”

Section: Introductionmentioning

confidence: 99%

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Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection

2022

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IntroductionThe study of early markers to detect kidney malfunction has increased in recent years since serum markers, such as creatinine increase when there is a 75% loss of renal mass. Urinary capillary electrophoresis (UCE) is an available laboratory technique that provides an easily interpretable electrophoretic pattern. This pattern in our study has been divided into five fractions as it is done in serum: fraction 1 migrating in the albumin zone, fraction 2 in the alpha1-globulins zone, fraction 3 in the alpha2-globulins zone, fraction 4 in the beta-globulins zone, and fraction 5 in the gamma globulins zone. UCE can be useful in the early diagnosis of renal disease.Material and methodsIn this study, UCE was performed in dogs with azotemia and proteinuria due to chronic kidney disease (CKD) not related to Leishmania infantum (L. infantum) infection (G1, n = 11) and dogs with CKD related to L. infantum infection (G2, n = 17) and compared with reference intervals from healthy dogs (G0, n = 123), with the aim of comparing their phoretograms and assessing changes in the fractions of the phoretograms based on the health status of individuals.ResultsFraction 2 was statistically augmented in dogs with CKD (G1) when compared with the healthy population (G0) and dogs infected by L. infantum (G2). Fraction 3 was statistically increased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 4 was found to be statistically decreased in dogs with CKD (G1) and dogs infected by L. infantum (G2) compared with G0. Fraction 5 was statistically higher in dogs with L. infantum (G2) compared with G0 and dogs with CKD (G1). No statistical relationship was found between the protein to creatinine ratio and different fractions from the urinary phoretogram in the study population. No statistical relationship was found between serum and urine fractions in the study population.DiscussionThe results of the present study suggest that UCE is a promising non-invasive technique that might be used as a part of the diagnostic and follow-up in dogs with kidney disease due to different pathologies.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection

2022

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“…In animal studies, the mice urine samples were analyzed to determine the influence of gut microbiota on the composition of uremic solute in CKD [105]. Pelander et al [106] studied the peptides in dog urine to find a link to CKD. In the latter study, urine samples from 25 dogs with CKD and 25 healthy dogs were used.…”

Section: F I G U R E 4 Study Design and Urinary Ce-ms Analysis Of Patients With Renal Cyst And Diabetes Syndrome (Rcad) (A)mentioning

confidence: 99%

“…Pelander et al. [ 106 ] studied the peptides in dog urine to find a link to CKD. In the latter study, urine samples from 25 dogs with CKD and 25 healthy dogs were used.…”

Section: Ce‐ms As a Tool For Metabolomic Studiesmentioning

confidence: 99%

Current applications of capillary electrophoresis‐mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid‐2021): A review

Helena

Voráčová

Řemínek

et al. 2021

J of Separation Science

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Capillary electrophoresis coupled online with mass detection is a modern tool for analyzing wide ranges of compounds in complex samples, including urine. Capillary electrophoresis with mass spectrometry allows the separation and identification of various analytes spanning from small ions to high molecular weight protein complexes. Similarly to the much more common liquid chromatographymass spectrometry techniques, the capillary electrophoresis separation reduces the complexity of the mixture of analytes entering the mass spectrometer resulting in reduced ion suppression and a more straightforward interpretation of the mass spectrometry data. This review summarizes capillary electrophoresis with mass spectrometry studies published between the years 2017 and 2021, aiming at the determination of various compounds excreted in urine. The properties of the urine, including its diagnostical and analytical features and chemical composition, are also discussed including general protocols for the urine sample preparation. The mechanism of the electrophoretic separation and the instrumentation for capillary electrophoresis with mass spectrometry coupling is also included. This review shows the potential of the capillary electrophoresis with mass spectrometry technique for the analyses of different kinds of analytes in a complex biological matrix. The discussed applications are divided into two main groups (capillary electrophoresis with mass spectrometry for the determination of drugs and drugs of abuse in urine and capillary electrophoresis with mass spectrometry for the studies of urinary metabolome).

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CE–MSApproaches for Peptidomics

Štěpánová¹,

Kašička²

2022

Capillary Electrophoresis‐Mass Spectrometry for Proteomics and Metabolomics

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Urinary peptidome analyses for the diagnosis of chronic kidney disease in dogs

Cited by 11 publications

References 33 publications

Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection

Urinary phoretograms performed by capillary electrophoresis in dogs with chronic disease with or without Leishmania infantum infection

Current applications of capillary electrophoresis‐mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid‐2021): A review

CE–MSApproaches for Peptidomics

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