<scp>CE–MS</scp>Approaches for Peptidomics

Štěpánová, Sille; Kašička, Václav

doi:10.1002/9783527833092.ch9

Capillary Electrophoresis‐Mass Spectrometry for Proteomics and Metabolomics 2022

DOI: 10.1002/9783527833092.ch9

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CE–MSApproaches for Peptidomics

Sille Štěpánová¹,

Václav Kašička²

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2023

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Cited by 2 publications

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“…Nowadays, CE methods are appreciated as recognized counterparts and/or complements of the (U)HPLC (highperformance liquid chromatography) and nano-LC methods [36][37][38] that are mostly used for both analytical and preparative peptide separations. In addition to the last one of the previous reviews [35], the recent developments of CE and CEC separations of peptides were reported in some other reviews [39][40][41][42], books [43,44], and book chap-ters [22,45,46] dealing with particular aspects of CE and MCE of peptides, proteins, amino acids (AAs), and other biomolecules.…”

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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Ion mobility in gas and liquid phases: How much orthogonality is obtained in capillary electrophoresis–ion mobility–mass spectrometry?

Schairer,

Plathe,

Hudelmaier

et al. 2023

Electrophoresis

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Ion mobility–mass spectrometry (IM–MS) is an ever‐evolving tool to separate ions in the gas phase according to electrophoretic mobility with subsequent mass determination. CE is rarely coupled to IM–MS, possibly due to similar separation mechanisms based on electrophoretic mobility. Here, we investigate the orthogonality of CE and ion mobility (IM) by analyzing a complex peptide mixture (tryptic digest of HeLa proteins) with trapped ion mobility mass spectrometry (TIMS–MS). Using the nanoCEasy interface, excellent sensitivity was achieved by identifying thousands of peptides and achieving a peak capacity of 7500 (CE: 203–323 in a 150 cm long capillary, IM: 27–31). Plotting CE versus mass and CE versus (inverse) mobility, a clear grouping in curved striped patterns is observed according to the charge‐to‐size and mass‐to‐charge ratios. The peptide charge in the acidic background electrolyte can be estimated from the number of basic amino acids, with a few exceptions where neighboring effects reduce the positive charge. A surprisingly high orthogonality of CE and IM is observed, which is obviously caused by solvation effects leading to different charges and sizes in the liquid phase compared to the gas phase. A high orthogonality of CE and ion mobility is expected to be observed for other peptide samples as well as other substance classes, making CE–IM–MS a promising tool for various applications.

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CE–MSApproaches for Peptidomics

Cited by 2 publications

References 119 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Ion mobility in gas and liquid phases: How much orthogonality is obtained in capillary electrophoresis–ion mobility–mass spectrometry?

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