Urinary isoprostane excretion is not confounded by the lipid content of the diet

Richelle, Myriam; Turini, Marco; Guidoux, R.; Tavazzi, Isabelle; Métairon, Sylviane; Fay, Laurent B.

doi:10.1016/s0014-5793(99)01259-4

Cited by 110 publications

(78 citation statements)

References 20 publications

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“…Although it had been stated in the past that the formation of isoprostanes was not affected by the lipid content of the diet (Richelle et al, 1999;Willcox et al, 2003), it was recently shown that the abundant amount of n-3 PUFA in the organism leads to the formation of the family of F 3 -isoprostanes and decreases F 2 -isoprostanes (Gao et al, 2006). However, attempts to measure F 3 -isoprostanes have been so far met with only limited success, especially with noninvasively collected samples such as urine (Adiyaman et al, 1998).…”

Section: Discussionmentioning

confidence: 95%

The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs

Frankič

Levart

Salobir

2010

Animal

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The objective of our study was to determine the antioxidative potential of a plant extract (PE) mixture composed of carvacrol, capsicum oleoresin and cinnamaldehyde against high n-3 polyunsaturated fatty acid (PUFA)-induced oxidative stress in young pigs. Thirty-two weaned castrated male crossbred pigs (BW 10.9 kg; n 5 32) were randomly assigned to four dietary treatments (n 5 8). The negative control diet (Cont) contained 17.2% energy from fat. Oxidative stress was induced in three of the four experimental groups with the inclusion of n-3 PUFA rich linseed oil. Linseed oil substituted wheat starch in the diet to elevate the amount of energy from fat to 34.1%. One of these diets served as a positive control (Oil), one was additionally supplemented with 271.2 mg/kg of PE mixture and one with 90.4 mg/kg a-tocopheryl acetate (Vit E). After 14 days of treatment, blood and urine were collected for the determination of lipid peroxidation and DNA damage. Lipid peroxidation was studied by plasma malondialdehyde (MDA) concentrations, 24 h urinary MDA and F 2 -isoprostane (iPF 2a -VI) excretion, total antioxidant status of plasma and glutathione peroxidase assays. Lymphocyte DNA fragmentation and 24 h urinary 8-hydroxy-2 0 -deoxyguanosine excretion were measured to determine DNA damage. Consumption of n-3 PUFA rich linseed oil increased the amount of MDA in plasma and urine, and induced DNA damage in lymphocytes, but did not elevate the amount of iPF 2a -VI excreted in the urine. The supplementation with PE and with Vit E did not reduce MDA levels in plasma and urine, but it decreased the percentage of DNA damage in lymphocytes ( P , 0.001). The PE reduced the urinary iPF 2a -VI excretion in comparison to the Cont diet. The results show that PE and Vit E supplemented to pigs in concentrations of 271.2 mg/kg and 90.4 mg/kg, respectively, can effectively protect pig's blood lymphocytes against oxidative DNA damage, thus suggesting their potentially beneficial effects on the immune system under dietary-induced oxidative stress.

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Section: Discussionmentioning

confidence: 95%

The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs

Frankič

Levart

Salobir

2010

Animal

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“…In addition, we have shown that urinary excretion of 8-epi-PGF 2␣ and plasma MDA correlate with other more commonly used adiposity indexes, such as BMI and WHR. Nevertheless, we found some specific relations between body fat measures and these two measures of lipid peroxidation that may result from differences in specificity and sensitivity in assessing lipid peroxidation (29,30). MDA is a general indicator of oxidative stress rather than a specific marker of lipid peroxidation and it is known to vary in response to the diet (23,24).…”

Section: Discussionmentioning

confidence: 97%

Visceral fat accumulation determines postprandial lipemic response, lipid peroxidation, DNA damage, and endothelial dysfunction in nonobese Korean men

Jang

Kim

Ryu

et al. 2003

Journal of Lipid Research

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Visceral fat has been associated with multiple cardiovascular disease (CVD) risk factors. The aim of this study was to identify anthropometrical measures most closely associated with some well-known CVD risk factors. Because most Asians at risk have normal body mass index (BMI) according to Western standards, we studied healthy nonobese Korean males (n ‫؍‬ 102; age: 36.5 ؎ 0.8 years, BMI: 23.8 ؎ 0.2 kg/m 2 ). Visceral fat area (VFA) at the fourth lumbar vertebra was associated with increased postprandial triglyceride (TG) response ( r ‫؍‬ 0.53, P Ͻ 0.001) and with plasma malondialdehyde (MDA) ( r ‫؍‬ 0.36, P Ͻ 0.01) and PGF2 ␣ ( r ‫؍‬ 0.24, P Ͻ 0.05). When matched for BMI and age, men with high VFA (HVFA) ( у 100 cm 2 ; n ‫؍‬ 27) had higher blood pressure ( P Ͻ 0.01), increased consumption of cigarettes ( P Ͻ 0.01), and lower ratio of energy expenditure to calorie intake ( P Ͻ 0.01) as compared with low VFA men ( Ͻ 100 cm 2 ; n ‫؍‬ 27). Men with HVFA showed higher TG, glucose, and insulin responses following fat and oral glucose tolerance tests respectively higher plasma concentrations of MDA ( P Ͻ 0.001), urinary PGF 2 ␣ ( P Ͻ 0.05), and lymphocytes deoxyribonucleic acid tail moments ( P Ͻ 0.01). Conversely, HVFA was associated with lower testosterone, insulin-like growth factor-1, and brachial artery flowmediated dilation ( P Ͻ 0.001). In conclusion, our data indicate that visceral fat accumulation, even in nonobese men, is a major factor contributing to increased CVD risk. The most recent figures about the epidemic of obesity and overweight in the US and other industrialized countries are appalling. Thirty-four percent of US adults are considered overweight, and an additional 31 percent are obese. The consequences of this are far beyond the esthetics of the population. Being overweight or obese increases the risk of hypertension, heart disease, stroke, diabetes, and some cancers. In the US alone, 300,000 people die each year due to obesity-related causes, making it the second-leading cause of death after smoking. Asians experience similar risk of obesity-related diseases, but the problem is more insidious, as the risk threshold at which body mass index (BMI) appears to trigger the disease may be much lower for Asians ( ‫ف‬ 23 kg/m 2 ) than for white populations (30 kg/m 2 ) (1). This may result from differences in body frame that affect the relation between body fat and BMI.The population of Korea, similar to other industrialized countries in Asia, is experiencing dramatic and fast changes in dietary and physical activity habits that are the driving force behind the increase in obesity and obesity-related diseases. Korean adults have increased the percent of calories from fat in their diets from 6% in 1969 to 19% in 1998. Despite the greater than 3-fold increase in the dietary fat intake, their daily calorie intake (about 1,950 kcal/d) and BMI (about 22.5 kg/m 2 ) have changed little over the same period (2). However, these lifestyle changes have dramatically affected the central adipose tissue distr...

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“…Oxidative lipid damage, referred to as lipid peroxidation, produces a gradual loss of cell membrane fluidity, reduces membrane potential, and increases permeability to ions like Ca 2+ . Oxidative stress has been proposed to be involved in the pathophysiology of many chronic diseases, including atherosclerosis (Gutteridge and Halliwell, 1990) and diabetes (Richelle et al;, and is known to accelerate ageing processes (De la Fuente and Victor, 2000).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of quercetin on the morphology of pancreatic β-cells of streptozotocin-treated diabetic rats

Adewole

Caxton-Martins²,

Ojewole³

2007

Afr. J. Trad. Compl. Alt. Med.

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This study was undertaken to investigate the protective effects of quercetin (QCT) on the morphology of pancreatic β-cells against diabetes mellitus and oxidative stress experimentally-induced by streptozotocin (STZ) treatment in Wistar rats. Fifty male and female Wistar rats (200-250 g) were randomly divided into three experimental groups (i. e., control, STZ-treated, and STZ + Quercetin-treated groups). Diabetes was induced in the diabetic groups (B and C) of animals, by a single intraperitoneal injection of STZ (75 mg/kg), while each of the rats in the 'control' group received equal volume of citrate buffer (pH 6.3) solution intraperitoneally. In group C rats, quercetin (QCT, 25 mg/kg/day i. p.) was injected daily for 3 days prior to STZ treatment, and QCT administration continued until the end of the study period (30 days). Diabetes mellitus was confirmed by using Bayer's Glucometer Elite ® and compatible blood glucose test strips. The rats were sacrificed serially until the end of the study period (after 30 days). The pancreases of the sacrificed rats were excised and randomly processed for histological staining and biochemical assays for antioxidant enzymes [such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and serum nitric oxide (NO)]. In the diabetic state, pancreatic β-cells of STZ-treated group B rats histologically demonstrated an early chromatin aggregation, cytoplasmic vesiculation in the central β-cells, nuclear shrinkage, and lysis of β-cells with distortion of granules. The morphology of QCT-treated rats' pancreases showed viable cellularity with distinct β-cell mass. STZ treatment significantly decreased (p<0.05) GSHPx, SOD, CAT and pancreatic insulin content. However, STZ treatment increased blood glucose concentrations, MDA and serum NO. The QCT-treated group of animals showed a significant decrease (p<0.05) in elevated blood glucose, MDA and NO. Furthermore, QCT treatment significantly increased (p<0.05) antioxidant enzymes' activities, as well as pancreatic insulin contents. Quercetin (QCT) treatment protected and preserved pancreatic β-cell architecture and integrity. In conclusion, the findings of the present experimental animal study indicate that QCT treatment has beneficial effects on pancreatic tissues subjected to STZ-induced oxidative stress by directly quenching lipid peroxides and indirectly enhancing production of endogenous antioxidants.

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Urinary isoprostane excretion is not confounded by the lipid content of the diet

Cited by 110 publications

References 20 publications

The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs

The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs

Visceral fat accumulation determines postprandial lipemic response, lipid peroxidation, DNA damage, and endothelial dysfunction in nonobese Korean men

Protective effect of quercetin on the morphology of pancreatic β-cells of streptozotocin-treated diabetic rats

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