The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs

Abstract

The objective of our study was to determine the antioxidative potential of a plant extract (PE) mixture composed of carvacrol, capsicum oleoresin and cinnamaldehyde against high n-3 polyunsaturated fatty acid (PUFA)-induced oxidative stress in young pigs. Thirty-two weaned castrated male crossbred pigs (BW 10.9 kg; n 5 32) were randomly assigned to four dietary treatments (n 5 8). The negative control diet (Cont) contained 17.2% energy from fat. Oxidative stress was induced in three of the four experimental groups with the inclusion of n-3 PUFA rich linseed oil. Linseed oil substituted wheat starch in the diet to elevate the amount of energy from fat to 34.1%. One of these diets served as a positive control (Oil), one was additionally supplemented with 271.2 mg/kg of PE mixture and one with 90.4 mg/kg a-tocopheryl acetate (Vit E). After 14 days of treatment, blood and urine were collected for the determination of lipid peroxidation and DNA damage. Lipid peroxidation was studied by plasma malondialdehyde (MDA) concentrations, 24 h urinary MDA and F 2 -isoprostane (iPF 2a -VI) excretion, total antioxidant status of plasma and glutathione peroxidase assays. Lymphocyte DNA fragmentation and 24 h urinary 8-hydroxy-2 0 -deoxyguanosine excretion were measured to determine DNA damage. Consumption of n-3 PUFA rich linseed oil increased the amount of MDA in plasma and urine, and induced DNA damage in lymphocytes, but did not elevate the amount of iPF 2a -VI excreted in the urine. The supplementation with PE and with Vit E did not reduce MDA levels in plasma and urine, but it decreased the percentage of DNA damage in lymphocytes ( P , 0.001). The PE reduced the urinary iPF 2a -VI excretion in comparison to the Cont diet. The results show that PE and Vit E supplemented to pigs in concentrations of 271.2 mg/kg and 90.4 mg/kg, respectively, can effectively protect pig's blood lymphocytes against oxidative DNA damage, thus suggesting their potentially beneficial effects on the immune system under dietary-induced oxidative stress.

Effects of Cinnamon (C. zeylanicum) Bark Oil Against Taxanes-Induced Damages in Sperm Quality, Testicular and Epididymal Oxidant/Antioxidant Balance, Testicular Apoptosis, and Sperm DNA Integrity

“…CBO is one of the most important volatile oils. Although there is no evidence about the effect of cinnamaldehyde, which is the major component of CBO used in this study, on sperm parameters, testis structure, and sperm DNA integrity, it has been reported that ingestion of plant extract containing cinnamaldehyde effectively protects pig lymphocytes against oxidative DNA damage (51). Oil and other products of C. zeylanicum have been reported to result in decrease in testicular lipid peroxidation levels, and increases in LH, FSH, and testosterone (52,53) concentrations; reproductive organ weights; sperm count and motility; antioxidant activities (GSH, GSH-Px, and CAT); and DST in healthy animals (28).…”

Effect of capsaicin and capsaicin plus DL-methionine hydroxy analog in diet on growth performance and gastrointestinal conditions of nursery pigs

“…Supplemental CS or CS+LMA in the diets (21 to 64 days of age) did not influence the body weight, feed intake and FCR of piglets (P>0.05). Positive effects of supplementing CS, DL-LMA or CS+LMA on growth performance and health of piglets have been observed (Kaewtapee et al 2009;Frankic et al 2010;Jarupan et al 2018). However, several reports showed that CS supplementation did not support the growth rate and feed utilization (Udompoka, 2006), and less positive response in growth performance from the supplementation of various feed additives has been also reported (Walsh et al 2012).…”

The effects of oral carvacrol treatment against H2O2induced injury on isolated pancreas islet cells of rats

“…The treatment with carvacrol restored the imbalance between ROS and antioxidant system and by the way prevented the fragmentation. 24 In conclusion, the viability of donor pancreas islets is protected with carvacrol treatment and carvacrol effectively protects of apoptosis. All of the steps were applied according to the manufacturer's instructions.…”