Urinary hyaluronic acid: a versatile marker of bladder cancer

El‐Hefnawy, Ahmed S.; Rizk, Ehsan; Khamis, Nabila Maher Al Demerdash; Barakat, Mohammed Ali Atwa; Khater, Sherry M.; Shokeir, Ahmed A.

doi:10.1007/s11255-020-02480-4

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…53 It is a potential method for bladder cancer detection, and the cut-off value is 500 ng/mL in urine. 54 The LOD of our study in HA was 8.3 pg/mL, which was lower than those done by ELISA, 53 capillary electrophoresis, 55 and liquid chromatography, 56 as shown in Table 2. The detection range of our work covers that of ELISA (sensing range: 1 ng/mL to 2.5 μg/ mL), 53,57 indicating that the AuNM sandwich immunoassay is a candidate method to diagnose bladder cancer via HA.…”

Section: Detection Of Bladder Cancercontrasting

confidence: 53%

“…Lokeshwar et al first demonstrated that the HA test has 83% sensitivity and 90% specificity for bladder cancer detection in 1997 and later combined HA with hyaluronidase to improve the accuracy in 2000 . It is a potential method for bladder cancer detection, and the cut-off value is 500 ng/mL in urine . The LOD of our study in HA was 8.3 pg/mL, which was lower than those done by ELISA, capillary electrophoresis, and liquid chromatography, as shown in Table .…”

Section: Resultsmentioning

confidence: 54%

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Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

Yang

Chang

Chiu

et al. 2023

ACS Appl. Nano Mater.

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Bladder cancer is one of the most common malignancies in the urinary system. Cystoscopy is the traditional standard diagnostic method for bladder cancer with subsequent biopsy or surgery. However, this method is uncomfortable for most patients because it requires anesthesia and possibly causes infections. Because of the high recurrence rate of bladder cancer, a rapid, low-cost, high-sensitivity, and noninvasive sensing method is needed. This study employed gold nanomushroom (AuNM) chips for bladder cancer biomarker detection, combining the benefits of sandwich immunoassay and localized surface plasmon resonance (LSPR) sensing. With a metal nanotransfer printing technique, which is cheap and straightforward, the AuNMs were patterned on flexible polycarbonate (PC) sheets. The gold caps stood above PC stems and provided ample spatial areas for capturing the biomarkers to be sensed. Three biomarkers served as the antigens and analytes, including human complement factor H (CFH), hyaluronic acid (HA), and nuclear matrix protein 22 (NMP22). Different antibodies, against the same biomarker, were covalently conjugated to AuNMs or gold nanoparticles, respectively. When the antibody–antigen–antibody sandwich structure formed, the plasmonic coupling between the AuNM surface and the gold nanoparticles significantly enhanced LSPR signals. The LSPR red shifts correlated quantitatively with the concentrations of the biomarkers. The limits of detection were 6.5, 8.3, and 7.0 pg/mL for CFH, HA, and NMP22, respectively. The chip’s specificity was tested and confirmed, excluding the nonspecific binding and false-positive possibility. The sensing performance of this sandwich immunoassay-based AuNM chip was better than that of the commercialized enzyme-linked immunosorbent assay. It provided a rapid, label-free, and easy operating platform for diagnosing and monitoring bladder cancer.

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Section: Detection Of Bladder Cancercontrasting

confidence: 53%

Section: Resultsmentioning

confidence: 54%

Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

Yang

Chang

Chiu

et al. 2023

ACS Appl. Nano Mater.

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“…The relationship between HA and bladder cancer is also strong. First, higher HA levels are associated with bladder cancer [ 58 , 59 ]. Second, the HA synthase gene HAS2 was found to have elevated expression in bladder cancer cells [ 59 , 60 , 61 ].…”

Section: Cd44 As a Marker For Bladder Cancer Growth And Progressionmentioning

confidence: 99%

CD44 in Bladder Cancer

Duex,

Theodorescu

2024

Cancers

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“…In china, its incidence ranks first among urogenital system tumors (3). Bladder cancer mainly includes bladder urothelial carcinoma, bladder squamous cell carcinoma and bladder adenocarcinoma (4). Early bladder cancer lacks significant symptoms, and a small number of patients develop hematuria, which is often in the advanced stage at the time of diagnosis (5).…”

Section: Introductionmentioning

confidence: 99%

Lamin B2 contributes to the proliferation of bladder cancer cells via activating the expression of cell division cycle‑associated protein 3

Chen

et al. 2022

Int J Mol Med

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Bladder cancer is the most common malignant tumor of the urinary system, and in China it is first among urogenital system tumors. More therapeutic targets are still urgently required to combat this disease. Lamin B2 (LMNB2) is a type of nuclear lamina filament protein, which is involved in multiple cellular processes, and known as an oncogene affecting the progression of multiple types of cancers. Although the multiple effects of LMNB2 on cancer progression have been elucidated, its possible role in bladder cancer remains unclear. In the present study, it was determined that LMNB2 expression was upregulated in human bladder cancer tissues, and its expression was correlated with the prognosis and the clinical features, including tumor stage (P=0.001) and recurrence (P= 0.006) of patients with bladder cancer. In addition, it was further revealed that LMNB2 depletion inhibited bladder cancer cell proliferation, stimulated cell cycle arrest and apoptosis in vitro, and suppressed tumor growth of bladder cancer cells in mice. Furthermore, the present data revealed that LMNB2 promoted the proliferation of bladder cancer cells via transcriptional activation of CDCA3 expression. Therefore, the role of LMNB2 in bladder cancer progression was demonstrated, and may serve as a promising therapeutic target for bladder cancer treatment.

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Urinary hyaluronic acid: a versatile marker of bladder cancer

Cited by 6 publications

References 29 publications

Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

CD44 in Bladder Cancer

Lamin B2 contributes to the proliferation of bladder cancer cells via activating the expression of cell division cycle‑associated protein 3

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