Lamin B2 contributes to the proliferation of bladder cancer cells via activating the expression of cell division cycle‑associated protein 3

Abstract

Bladder cancer is the most common malignant tumor of the urinary system, and in China it is first among urogenital system tumors. More therapeutic targets are still urgently required to combat this disease. Lamin B2 (LMNB2) is a type of nuclear lamina filament protein, which is involved in multiple cellular processes, and known as an oncogene affecting the progression of multiple types of cancers. Although the multiple effects of LMNB2 on cancer progression have been elucidated, its possible role in bladder cancer remains unclear. In the present study, it was determined that LMNB2 expression was upregulated in human bladder cancer tissues, and its expression was correlated with the prognosis and the clinical features, including tumor stage (P=0.001) and recurrence (P= 0.006) of patients with bladder cancer. In addition, it was further revealed that LMNB2 depletion inhibited bladder cancer cell proliferation, stimulated cell cycle arrest and apoptosis in vitro, and suppressed tumor growth of bladder cancer cells in mice. Furthermore, the present data revealed that LMNB2 promoted the proliferation of bladder cancer cells via transcriptional activation of CDCA3 expression. Therefore, the role of LMNB2 in bladder cancer progression was demonstrated, and may serve as a promising therapeutic target for bladder cancer treatment.

Pan-Cancer TCGA Analysis Reveals the Potential Involvement of Lamin B2 in Dysregulating Chromosome Segregation in Human Cancer

“…CDC20 plays a major role in proper chromosome segregation and promoting mitosis (Kapanidou et al 2017) and has been recently reported as a novel biomarker for epithelial ovarian cancer (Xi et al 2022). Recently, Ji et al (2022) reported that lamin B2 mediates the proliferation of bladder cancer cells through activation of cell division cycle associated 3 (CDCA3), which is highly consistent with our data (Ji et al 2022). On the other hand, CDCA8, an important member of the chromosomal passenger complex (CPC complex), is involved in proper chromosome segregation and has also been reported to overexpress in malignant tumors.…”

“…Dong et al (2021) reported that lamin B2 promotes tumorigenesis in colorectal cancer by regulating p21 (Dong et al 2021). In another study, Ji et al (2022) reported that lamin B2 transcriptionally activates cell division cycle-associated protein 3 (CDCA3) in bladder cancer and thereby controls the proliferation of bladder cancer cells (Ji et al 2022). In order to gain a clear understanding of the functionality of lamin B2 in driving cancer, we have identi ed which of the lamin B2 binding partners are co-expressed with lamin B2 in all cancers.…”

“…For instance, lamin B1 expression is elevated in hepatocellular carcinoma (Lim et al 2002;Sun et al 2010; Wong and Luk 2012) and prostate cancer (Luo et al 2021), while the downregulation of the lamin B1 is also reported for multiple cancers such as gastric cancer ( signi cance and potential functions of lamin B1 in pan-cancer based on TCGA data analysis (Qin et al 2022). Similar to lamin B1, lamin B2 has also been reported in cancers such as the bladder (Ji et al 2022), colorectal (Dong et al 2021), and triple-negative breast cancer (Zhao et al 2021). Nevertheless, there is no systematic study that showcases the involvement of lamin B2 in pan-cancer and its prognostic signi cance.…”

A Lamin Family-Based Signature Predicts Prognosis and Immunotherapy Response in Hepatocellular Carcinoma

“…Knockdown of LMNB1 was functionally relevant for melanoma and lung adenocarcinoma (LUAD) progression by influencing cell senescence and DNA damage [16,17]. Upregulation of LMNB2 expression promoted cancer cell proliferation and indicated worse disease-free survival in colorectal cancer and bladder cancer [18,19]. Moreover, lamin family genes play functional roles in the immune system.…”