Urinary heavy metals, DNA methylation, and subclinical atherosclerosis

Lin, Chien‐Yu; Lee, Hui‐Ling; Hwang, Yi‐Ting; Huang, Po-Chin; Wang, Chikang; Sung, Fung‐Chang; Wu, Chunchi; Su, Ta‐Chen

doi:10.1016/j.ecoenv.2020.111039

Cited by 24 publications

(12 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The review by Tinkov et al [8] included one study on carotid artery intima-media thickness (cIMT) [54], two studies from Sweden of carotid artery plaque [55][56], and another Swedish study of both plaque and cIMT [57]. After this review was conducted, Lin et al [58] published a study on cIMT in young (12-30 years) Taiwanese individuals. In addition, the first study of coronary artery atherosclerosis (estimated as coronary artery calcium score [CACS]) was published recently [59].…”

Section: Asymptomatic Atherosclerosis In Carotid or Coronary Arteriesmentioning

confidence: 99%

See 1 more Smart Citation

Review of cadmium exposure and smoking‐independent effects on atherosclerotic cardiovascular disease in the general population

Fagerberg

Barregård

2021

J Intern Med

View full text Add to dashboard Cite

show abstract

Section: Asymptomatic Atherosclerosis In Carotid or Coronary Arteriesmentioning

confidence: 99%

“…After this review was conducted, Lin et al. [58] published a study on cIMT in young (12–30 years) Taiwanese individuals. In addition, the first study of coronary artery atherosclerosis (estimated as coronary artery calcium score [CACS]) was published recently [59].…”

Section: Part I: Epidemiological Studies On Cadmium and Ascvdmentioning

confidence: 99%

Review of cadmium exposure and smoking‐independent effects on atherosclerotic cardiovascular disease in the general population

Fagerberg

Barregård

2021

J Intern Med

View full text Add to dashboard Cite

show abstract

“…Meanwhile, variations in DNA methylation are associated with stressful conditions that are known to affect cell proliferation 15 . In particular, many heavy metals can alter DNA methylation, that is, causing hypermethylation or hypomethylation 16 . Cd‐induced toxicity has normally been related to a global DNA hypermethylation or over‐expression of DNA methyltransferases (DNMTs) 17 .…”

Section: Introductionmentioning

confidence: 99%

Low‐dose cadmium exposure facilitates cell proliferation by promoter hypermethylation of RASSF1A and DAPK1 genes

Zhang

Tang

et al. 2021

Environmental Toxicology

View full text Add to dashboard Cite

Cadmium (Cd) at low concentrations has a potential to promote cell proliferation. However, the molecular mechanisms of Cd‐induced proliferation are not well understood. Here, we reported that Cd (0–500 nM) significantly promoted the proliferation of HepG2 cells as demonstrated by elevated cell viability, more EdU‐positive cells and increased gene expression of KI‐67 and COX‐2. Meanwhile, the gene expression of DNA methyltransferases was found to be elevated while that of tumor suppressor genes DAPK1 and RASSF1A were decreased under Cd exposure. Correspondingly, the methylation level of promoters in DAPK1 and RASSF1A were increased. Specifically, the CpG sites at −461 (Chr3:50, 374, 481) of RASSF1A promoter, and that at −260 (Chr9:90, 113, 207), −239 (Chr9:90, 113, 228), and −68 (Chr9:90, 113, 399) of DAPK1 promoter, were significantly hypermethylated. Moreover, 5‐azacytidine (an inhibitor of DNA methyltransferase) partly impaired Cd‐induced promoter hypermethylation of RASSF1A and DAPK1 genes, increased their expressions and slowed down Cd‐induced cell proliferation, suggesting that DNA methylation play an essential part in Cd‐boosted proliferation. The study showed that Cd caused promoter hypermethylation of RASSF1A and DAPK1, decreasing their expression and leading to higher level of cell proliferation. Furthermore, Cd at low concentrations could influence DNA methylation, which may serve as the proliferative mechanism of Cd.

show abstract

“…The cardiovascular system is one of the most important targets of Pb toxicity [27,28]. The entry of Pb into the cardiovascular system has been suggested to occur via various calcium (Ca 2+ ) channels [29,30], and Pb impairs the normal function of cardiac contraction [27,31,32]. We used the improved Met-lead 1.44 M1 Pb biosensor in iPSC-derived cardiomyocytes to demonstrate the sensing ability of Met-lead (FRET ratio increase; Figure 2F,G; original fluorescent images of Met-lead 1.44 M1 in YFP and CFP channels are shown in Figure S2) while simultaneously obtaining physiological parameters (e.g., the cardiomyocyte beating rate; Figure 2D,E).…”

Section: Simultaneous Monitoring Of Physical Cardiomyocyte Activity and Pb Contentmentioning

confidence: 99%

Versatile Cell and Animal Models for Advanced Investigation of Lead Poisoning

Yang

Chang²

2021

Biosensors

View full text Add to dashboard Cite

The heavy metal, lead (Pb) can irreversibly damage the human nervous system. To help understand Pb-induced damage, we applied a genetically encoded Förster resonance energy transfer (FRET)-based Pb biosensor Met-lead 1.44 M1 to two living systems to monitor the concentration of Pb: induced pluripotent stem cell (iPSC)-derived cardiomyocytes as a semi-tissue platform and Drosophila melanogaster fruit flies as an in vivo animal model. Different FRET imaging modalities were used to obtain FRET signals, which represented the presence of Pb in the tested samples in different spatial dimensions. Using iPSC-derived cardiomyocytes, the relationship between beating activity (20–24 beats per minute, bpm) determined from the fluctuation of fluorescent signals and the concentrations of Pb represented by the FRET emission ratio values of Met-lead 1.44 M1 was revealed from simultaneous measurements. Pb (50 μM) affected the beating activity of cardiomyocytes, whereas two drugs that stop the entry of Pb differentially affected this beating activity: verapamil (2 μM) did not reverse the cessation of beating, whereas 2-APB (50 μM) partially restored this activity (16 bpm). The results clearly demonstrate the potential of this biosensor system as an anti-Pb drug screening application. In the Drosophila model, Pb was detected within the adult brain or larval central nervous system (Cha-gal4 > UAS-Met-lead 1.44 M1) using fast epifluorescence and high-resolution two-photon 3D FRET ratio image systems. The tissue-specific expression of Pb biosensors provides an excellent opportunity to explore the possible Pb-specific populations within living organisms. We believe that this integrated Pb biosensor system can be applied to the prevention of Pb poisoning and advanced research on Pb neurotoxicology.

show abstract

Urinary heavy metals, DNA methylation, and subclinical atherosclerosis

Cited by 24 publications

References 55 publications

Review of cadmium exposure and smoking‐independent effects on atherosclerotic cardiovascular disease in the general population

Review of cadmium exposure and smoking‐independent effects on atherosclerotic cardiovascular disease in the general population

Low‐dose cadmium exposure facilitates cell proliferation by promoter hypermethylation of RASSF1A and DAPK1 genes

Versatile Cell and Animal Models for Advanced Investigation of Lead Poisoning

Contact Info

Product

Resources

About