Urinary exosomal mRNA detection using novel isothermal gene amplification method based on three-way junction

Moon, Jeong; Lim, Jaewoo; Lee, Seo-Young; Son, Ho‐Young; Rho, Hyun Wook; Kim, Hongki; Kang, Hyunju; Jeong, Jinyoung; Lim, Eun‐Kyung; Jung, Juyeon; Huh, Yong Min; Park, Hyun Gyu; Kang, Taejoon

doi:10.1016/j.bios.2020.112474

Cited by 20 publications

(11 citation statements)

References 40 publications

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“…After effective pre-enrichment (Figure a) or high selection of EVs (Figure b), targets in EVs can be detected directly in situ using nucleic acid probes. To enable signal output, the probes are typically labeled with radioisotopes, epitopes, biotin, or fluorophores, and combined with signal amplification techniques to improve sensitivity. − …”

Section: Detection Methods Of Ev Cargomentioning

confidence: 99%

Analysis and Biomedical Applications of Functional Cargo in Extracellular Vesicles

et al. 2022

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Extracellular vesicles (EVs) can facilitate essential communication among cells in a range of pathophysiological conditions including cancer metastasis and progression, immune regulation, and neuronal communication. EVs are membrane-enclosed vesicles generated through endocytic origin and contain many cellular components, including proteins, lipids, nucleic acids, and metabolites. Over the past few years, the intravesicular content of EVs has proven to be a valuable biomarker for disease diagnostics, involving cancer, cardiovascular diseases, and central nervous system diseases. This review aims to provide insight into EV biogenesis, composition, function, and isolation, present a comprehensive overview of emerging techniques for EV cargo analysis, highlighting their major technical features and limitations, and summarize the potential role of EV cargos as biomarkers in disease diagnostics. Further, progress and remaining challenges will be discussed for clinical diagnostic outlooks.

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Section: Detection Methods Of Ev Cargomentioning

confidence: 99%

Analysis and Biomedical Applications of Functional Cargo in Extracellular Vesicles

et al. 2022

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“…mRNA is a single-stranded molecule of RNA that is complementary to the genetic sequence of a gene and has been commonly used as a biomarker for early detection and treatment of cancer due to its humoral stability and biological regulatory function. It is usually quantitatively detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) [35][36][37]. Although qRT-PCR is a very sensitive method, it is timeconsuming and requires heavy instruments for the associated thermocycling reactions.…”

Section: Messenger Rna (Mrna)mentioning

confidence: 99%

“…Therefore, isothermal gene amplification [36,38], ECL [39], fluorescence [40,41], electrochemistry [42,43], and surface plasmon resonance (SPR) [44] have been developed as an alternative to PCR for mRNA detection.…”

Section: Messenger Rna (Mrna)mentioning

confidence: 99%

Nanomaterial-Based Fluorescence Resonance Energy Transfer (FRET) and Metal-Enhanced Fluorescence (MEF) to Detect Nucleic Acid in Cancer Diagnosis

Choi

Shin

et al. 2021

Biomedicines

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Nucleic acids, including DNA and RNA, have received prodigious attention as potential biomarkers for precise and early diagnosis of cancers. However, due to their small quantity and instability in body fluids, precise and sensitive detection is highly important. Taking advantage of the ease-to-functionality and plasmonic effect of nanomaterials, fluorescence resonance energy transfer (FRET) and metal-enhanced fluorescence (MEF)-based biosensors have been developed for accurate and sensitive quantitation of cancer-related nucleic acids. This review summarizes the recent strategies and advances in recently developed nanomaterial-based FRET and MEF for biosensors for the detection of nucleic acids in cancer diagnosis. Challenges and opportunities in this field are also discussed. We anticipate that the FRET and MEF-based biosensors discussed in this review will provide valuable information for the sensitive detection of nucleic acids and early diagnosis of cancers.

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“…Mass spectrometry analyses of breast cancer cell-line-delivered exosomes and patient-derived scaffolds revealed that exosomes are a rich source of breast-cancer-related proteins and surface biomarkers that may be used for disease diagnosis and prognosis [125][126][127]. Exosomes are very promising non-invasive biomarkers not only because of the high spectrum of information they may deliver, but also because they can be found in all body fluids, including urine [128].…”

Section: Exosomesmentioning

confidence: 99%

How to Predict Metastasis in Luminal Breast Cancer? Current Solutions and Future Prospects

Tabor

Szostakowska-Rodzoś

Fabisiewicz

et al. 2020

IJMS

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Breast cancer metastasis is the main cause of breast cancer mortality. Luminal breast cancer represents the majority of breast cancer cases and, despite relatively good prognosis, its heterogeneity creates problems with a proper stratification of patients and correct identification of the group with a high risk of metastatic relapse. Current prognostic tools are based on the analysis of the primary tumor and, despite their undisputed power of prediction, they might be insufficient to foresee the relapse in an accurate and precise manner, especially if the relapse occurs after a long period of dormancy, which is very common in luminal breast cancer. New approaches tend to rely on body fluid analyses, which have the advantage of being non-invasive and versatile and may be repeated and used for monitoring the disease in the long run. In this review we describe the current, newly-developed, and only-just-discovered methods which are or may become useful in the assessment of the probability of the relapse.

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Urinary exosomal mRNA detection using novel isothermal gene amplification method based on three-way junction

Cited by 20 publications

References 40 publications

Analysis and Biomedical Applications of Functional Cargo in Extracellular Vesicles

Analysis and Biomedical Applications of Functional Cargo in Extracellular Vesicles

Nanomaterial-Based Fluorescence Resonance Energy Transfer (FRET) and Metal-Enhanced Fluorescence (MEF) to Detect Nucleic Acid in Cancer Diagnosis

How to Predict Metastasis in Luminal Breast Cancer? Current Solutions and Future Prospects

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