Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats

Belenguer, Álvaro; Yañez, David; Balcells, J.; Baber, Nadide Hulya Ozdemir; Ronquillo, Manuel González

doi:10.1016/s0301-6226(02)00081-7

Cited by 54 publications

(78 citation statements)

References 17 publications

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“…Endogenous contribution to urinary PD The isotopic ( 15 N) dilution of urinary PD in relation to duodenal PB confirmed an endogenous fraction in the urinary excretion (Table 4), which has been observed in non-isotopic studies of goats (Lindberg, 1989;Belenguer et al, 2002), other ruminants (Balcells et al, 1991;Orellana-Boero et al, 2001;Guerouali et al, 2004) and non-ruminant species (Martín Orú e et al, 1995;Balcells et al, 1998). Differences in estimates of the endogenous fraction may partly reflect the different experimental techniques adopted.…”

Section: Discussionsupporting

confidence: 59%

“…Like sheep, goats exhibited low tissue xanthine-oxidase activity (Belenguer et al, 2002), which suggests a significant salvage of exogenous PD. That salvage pathway implies isotope enrichment of tissue PD, which might have affected our results, but it should be a time-dependent function.…”

Section: Discussionmentioning

confidence: 98%

“…In previous experience in our laboratory Granadina goats showed problems after surgery (Belenguer et al, 2002); therefore, in the present assay, a simple infusion catheter was placed into the proximal duodenum, rather than using cannulated animals. Nevertheless, the dry matter intake recorded in our study was lower and more variable than expected.…”

Section: Discussionmentioning

confidence: 99%

“…Differences in estimates of the endogenous fraction may partly reflect the different experimental techniques adopted. Lindberg (1991) estimated the endogenous fraction in milk-fed goat kids (242 mmol/kg LW 0.75 ), and Belenguer et al (2002) did so by using fasted goats (202.2 mmol/kg LW 0.75 ). However, those and other procedures, such as intragastric nutrition (Ørskov et al, 1979) or duodenal digesta draining (Balcells et al, 1991), are too aggressive to use with lactating animals.…”

Section: Discussionmentioning

confidence: 99%

“…lactation, of animals can affect the metabolism of PB . The recovery of duodenal PB and endogenous excretion have been investigated in non-lactating goats (Belenguer et al, 2002) but not in lactating animals. The aim of this study was to define the response model between the duodenal absorption of PB and their urinary excretion as PD in dairy goats.…”

Section: Introductionmentioning

confidence: 99%

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Modelling purine derivative excretion in dairy goats: endogenous excretion and the relationship between duodenal input and urinary output

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To determine the endogenous contribution of purine derivatives (PD) to renal excretion and the urinary recovery of duodenal purine bases (PB), five dairy Granadina goats (initial weight 6 s.e.: 38.6 6 2.78 kg) were each fitted with a duodenal infusion catheter. Animals were offered ad libitum a mixed diet (75 : 25; alfalfa hay : concentrate), which was supplied in equal portions every 3 h. To label microbial PB, ( 15 NH 4 ) 2 SO 4 was added to the concentrate. The lower enrichment of urinary PD ( 15 N-allantoin) compared with duodenal PB enrichment confirmed the presence of an endogenous PD fraction (268.5 6 21.98 mmol/kg weight 0.75 or 0.386 of the total PD excretion). The recovery of PD in urine and milk increased linearly in response to increasing amounts of duodenally infused RNA (starting on day 21 after parturition). On average, 0.74 of infused PB from RNA was recovered in urine. Milk PD constituted a minor (,0.01) fraction of the total PD excretion and this fraction decreased as the amount of infused PB increased. Our findings indicate that lactation in goats did not affect the urinary recovery of duodenal PB but increased the endogenous contribution to urinary excretion of PD.

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