Urinary excretion of pseudouridine and purine metabolites in ruminants

Puchała, R.; Shelford, J. A.; Barej, W.; Kulasek, G.; Pior, Hanna; Keyserlingk, M.A.G. von; Makoni, N.F.

doi:10.1111/j.1439-0396.1993.tb00804.x

Cited by 15 publications

(18 citation statements)

References 15 publications

(4 reference statements)

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“…There was no much difference in the body weight of the animals used in this study and they had similar excretion of urinary creatinine. The daily mean urinary creatinine excretion observed was lower than previous reports of between 112 to 117 mmol/d in European cattle (Puchala et al, 1993). It is conceivable that between breed/species differences in musculature and variations in body weight could account for these discrepancies.…”

Section: Purine Derivatives and Creatinine In Urine And Plasma At Difcontrasting

confidence: 75%

Effect of levels of feed intake on plasma concentration and urinary excretion of purine derivatives in crossbred bulls

George¹,

Dipu²,

Mehra³

et al. 2007

J. Anim. Feed Sci.

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The study evaluated the potential of the plasma concentration of purine derivatives (PD) as an alternative to total urine collection method to predict the microbial nitrogen (MN) supply in crossbred bulls. In a completely randomized design, 15 growing crossbred bulls were divided into three groups and assigned three levels (100, 80 and 60%) of voluntary dry matter intake (VDMI) on a diet of wheat straw and concentrate mixture (50:50). A metabolism trial of ten days duration was conducted after 90 days of experimental feeding. The daily urinary excretion of allantoin and PD decreased significantly (P<0.01) with the reduction in feed intake while creatinine excretion remained similar in animals fed at different levels. The MN supply calculated from the PD excreted in total urine (40.16 to 68.08 g/d) was higher (P<0.01) at increased levels of feed intake. The concentration of PD in plasma reflected the differences in MN supply among animals fed at 100 and 80% of VDMI, while it could not predict the differences in same among animals fed at 80 and 60% of VDMI. Daily glomerular filtration rate (GFR) was found to be similar in animals fed at 100 and 80% of VDMI, while there was a fall in GFR, when feed intake was lowered to 60% of VDMI. The results of this study revealed that, if the plasma concentration of PD is to be used as an index of microbial protein supply, the variation in GFR needs to be considered. Therefore, PD measurement in total urine is a better indicator of MN supply than the plasma PD concentration.

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Section: Purine Derivatives and Creatinine In Urine And Plasma At Difcontrasting

confidence: 75%

Effect of levels of feed intake on plasma concentration and urinary excretion of purine derivatives in crossbred bulls

George¹,

Dipu²,

Mehra³

et al. 2007

J. Anim. Feed Sci.

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“…Urinary concentrations of pseudouridine (Table 1) were similar to the 2.2 mmol/day of the non-lactating cows measured by Puchala et al (1993); however, plasma pseudouridine concentrations were not in the range of Rosskopf et al (1991), who found 0.006 ± 0.0016 mmol L − 1 circulating in bovine blood plasma. The cattle used in the study of Rosskopf et al (1991) were milking cattle and not rapidly growing beef steers, and this may account for the discrepancy.…”

Section: Discussionsupporting

confidence: 50%

“…Pseudouridine is a nucleoside isomer of uridine that exists in ribonucleic acids as a carbon-carbon linked ribonucleside on the TφC arm of transfer RNA (tRNA) (Lehninger, 1982) and it is released without further processing when tRNA is degraded (Weissman et al, 1962). Puchala et al (1993) validated the use of pseudouridine as an indicator of tRNA degradation by showing that infusion of RNA into the rumen did not increase its excretion in the urine over 24 h. Although not a specific indicator of tRNA degradation in skeletal muscle, the amount of pseudouridine excreted in urine over 24 h is useful as a metabolic indicator of whole body protein synthesis.…”

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confidence: 97%

Creatinine and pseudouridine in plasma and urine from Brahman-cross steers fed a low, medium or high plane of nutrition

2008

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“…Urinary allantoin has been studied more thoroughly in domestic ruminants, whose 24-h excretion was also positively correlated with energy intake over short time periods when there was little change in physical condition (Chen et al 1990a(Chen et al , 1990b(Chen et al , 1992Verbic et al 1990;Giesecke et al 1993Giesecke et al , 1994Puchala et al 1993). Cattle and sheep fed maintenance diets have shown pronounced species differences in endogenous allantoin excretion, and the effects of short-term fasting on allantoin excretion have been inconsistent Faichney 1964a, 1964b;Rys et al 1975;Antoniewicz and Pisulewski 1982;Fujihara et al 1987;Chen et al 1990aChen et al , 1990bPuchala and Kulasek 1991).…”

Section: Introductionmentioning

confidence: 99%

Prolonged winter undernutrition and the interpretation of urinary allantoin:creatinine ratios in white-tailed deer

DelGiudice¹,

Kerr²,

Mech³

et al. 2000

Can. J. Zool.

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The urinary allantoin:creatinine (A:C) ratio (expressed in micromoles of allantoin to micromoles of creatinine) has shown potential as an index of recent winter energy intake in preliminary controlled studies of elk (Cervus elaphus) involving mild condition deterioration (up to 11% loss of body mass). To ensure reliable nutritional assessments of free-ranging cervids by measuring A:C ratios of urine in snow, it is essential to extend this work. We assessed the effect of moderate and severe winter nutritional restriction on urinary A:C ratios of captive white-tailed deer (Odocoileus virginianus) that lost up to 32% body mass and related these ratios to metabolizable energy intake (MEI), body-mass loss, and other reported nutritional indicators. Deer in the control group were fed a low-protein, low-energy diet ad libitum, whereas deer in the treatment group were fed restricted amounts of the same diet. MEI was below the winter maintenance requirement for all deer, but was lower (P = 0.029) in treatment deer than in control deer. Percent body-mass loss differed between the two groups as the study progressed, and represented the full range of physiological tolerance (0-32% loss). Mean A:C ratios of control deer, which lost up to 17.4% body mass, showed a slight increasing (P = 0.086) trend, whereas initially similar A:C ratios of severely restricted deer increased (P = 0.0002) markedly by the eighth week (0.52 vs. 0.09 µmol:µmol). The urinary A:C ratio was not related (P = 0.839) to recent (2 days prior to urine sampling) MEI, but there was a marginally significant relation (r2 = 0.42, P = 0.110) between the A:C ratio and cumulative percent mass loss. The urinary A:C ratio was directly related to urinary urea nitrogen:creatinine (r2 = 0.59, P < 0.0001) and 3-methylhistidine:creatinine (r2 = 0.43, P < 0.0001) ratios. This study confirms that elevated and increasing A:C ratios may be due either to increasing energy intake or to accelerated tissue catabolism and increased endogenous contributions to urinary allantoin excretion.

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Urinary excretion of pseudouridine and purine metabolites in ruminants

Cited by 15 publications

References 15 publications

Effect of levels of feed intake on plasma concentration and urinary excretion of purine derivatives in crossbred bulls

Effect of levels of feed intake on plasma concentration and urinary excretion of purine derivatives in crossbred bulls

Creatinine and pseudouridine in plasma and urine from Brahman-cross steers fed a low, medium or high plane of nutrition

Prolonged winter undernutrition and the interpretation of urinary allantoin:creatinine ratios in white-tailed deer

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