Urinary excretion of 8-hydroxy-2′-deoxyguanosine measured by high-performance liquid chromatography with electrochemical detection

Pilger, Alexander; Ivancsits, Sabine; Germadnik, D.; Rüdiger, H. W.

doi:10.1016/s0378-4347(01)00449-2

Cited by 75 publications

(66 citation statements)

References 39 publications

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“…The calibration curve for the standard compound was linear (r 2 > 0.999) for concentrations in the range of 0.2-100 nmol/L (LLOQ: 0.2 nmol/L), which covers the lower range of the reported levels of 8-OHdG in human urine [29], and the slope was 0.00630±0.00026 (mean±S.D. RSD, 4.1%).…”

Section: Calibration Curve and Validationmentioning

confidence: 76%

“…The ELISA method suffers from the problem of non-selectivity because the antibody may cross-react with other substances present in urine [12,27,28]. HPLC-ECD has often been used [29], but it suffers from possible interference from the biological matrix, incompatibility of a stable isotope labeled internal standard [16,21]. For GC/MS analysis, 8-OHdG must be purified by HPLC and derivatized before analysis [15,16,28].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of 8-hydroxy-2′-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

Hosozumi

Toriba

Chuesaard

et al. 2012

Journal of Chromatography B

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Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a widely used noninvasive biomarker of oxidative stress. A selective, sensitive and rapid method for determining 8-OHdG in human urine was developed using hydrophilic interaction chromatographytandem mass spectrometry (HILIC-MS/MS) with electrospray ionization. 8-OHdG and isotopically labeled 8-OHdG (internal standard) were separated on a HILIC column with a mobile phase of 10 mM ammonium acetate : acetonitrile (1 : 9, v/v) within 10 min and detected by using a positive electrospray ionization interface under the selected reaction monitoring mode. The detection limits of 8-OHdG (corresponding to a signal-to-noise ratio of 3) for the HILIC-MS/MS system and the conventional method using a reversed-phase column with MS/MS were 1.0 and 26.0 fmol/injection, respectively. The proposed method makes it possible to monitor the basal level of urinary 8-OHdG from non-exposed healthy subjects and can be used for large-scale human studies.

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Section: Calibration Curve and Validationmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Analysis of 8-hydroxy-2′-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

Hosozumi

Toriba

Chuesaard

et al. 2012

Journal of Chromatography B

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“…One of the most frequently used biomarkers for •OH is 8-OHdG (15)(16)(17). However, their use should be limited, because only the amount of •OH formed inside nuclei and mitochondria can be shown (1,17).…”

Section: Daily Amount Of •Oh Scavenged By Deoxyguanosine (Dg) and Crnmentioning

confidence: 99%

“…However, their use should be limited, because only the amount of •OH formed inside nuclei and mitochondria can be shown (1,17). Furthermore, before the determination of 8-OHdG in urine, there are several reaction-steps from 8-OHdG-containing nucleotides.…”

Section: Daily Amount Of •Oh Scavenged By Deoxyguanosine (Dg) and Crnmentioning

confidence: 99%

“…Since we know that urinary (CTL + MG) and DMC are roughly in a one to one ratio (1,5), we estimated the amount of DMC from the corresponding measured amount of CTL plus MG: the total sum might be nearly equal to 2 × (CTL + MG). Furthermore, we wanted to show in this mini-review that the wellknown urinary level of 8-hydroxydeoxyguanosine (8-OHdG) ( Figure 1B) (17,18). However, we wanted to cite papers reporting that 8-OHdG could be a useful marker of •OH inside the nucleus and mitochondria (19).…”

Section: Introductionmentioning

confidence: 99%

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Daily hydroxyl radical scavenging capacity of mammals

Ienaga

Park

Yokozawa

2014

DD&T

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8‐Hydroxy‐2′‐deoxyguanosine as a marker of oxidativeDNAdamage related to occupational and environmental exposures [BATValue Documentation, 2010]

2016

The MAK‐Collection for Occupational Health and Safety

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Published in the series BAT Value Documentations , Vol. 5 (2010) The article contains sections titled: Introduction Methods for the measurement of 8‐ OHdG Determination of 8‐ OHdG in DNA High performance liquid chromatography ( HPLC ) with electrochemical ( EC ) detection Gas chromatography with mass spectrometry 32 P ‐postlabeling Methods based on the use of formamidopyrimidine DNA N‐glycosylase Enzyme‐linked immunosorband assay Determination of 8‐ OHdG in urine Use of 8‐ OHdG in occupational and environmental exposure studies Smoking Benzene, toluene, styrene and xylenes Asbestos Silica Chromium and cobalt Fine particulate matter Polycyclic aromatic hydrocarbons Reliability of 8‐ OHdG as a biomarker of oxidative stress in humans Conclusion

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Urinary excretion of 8-hydroxy-2′-deoxyguanosine measured by high-performance liquid chromatography with electrochemical detection

Cited by 75 publications

References 39 publications

Analysis of 8-hydroxy-2′-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

Analysis of 8-hydroxy-2′-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

Daily hydroxyl radical scavenging capacity of mammals

8‐Hydroxy‐2′‐deoxyguanosine as a marker of oxidativeDNAdamage related to occupational and environmental exposures [BATValue Documentation, 2010]

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