Urinary cholesterol excretion in men with benign prostatic hyperplasia and carcinoma of the prostate

Abstract

Urinary excretion of nonesterified (NEC) and total cholesterol (TC) has been investigated in 79 patients with prostatic adenoma (BPH) and 48 patients with carcinoma of the prostate. Normal range of NEC was determined in 62 healthy individuals and was found to be 0.26-2.2 mg/24 hours (2 SD) with a mean value of 0.76 mg/24 hours. TC ranged from 0.3-2.9 mg124 hours with a mean value of 0.92 mg/24 hours. In benign prostatic hyperplasia normal values for NEC and TC were determined in stages without residual urine. in prostatic cancer has been reported re~e n t l y .~* " It was concluded that both parameters are valuable in the diagnosis of the disease. A comparative evaluation of NEC and TC has not yet been undertaken; in addition, the performed studies did not show the value of these parameters in diagnosis of early clinical stages. Other known common conditions of urinary cholesterol hyperexcretion in males are pluripotential testicular neoplasms, diseases of kidney and urinary tract and benign prostatic h y p e r p l a~i a .~J~~'~,~* J~ In regard to the frequency of BPH in comparison to prostatic carcinoma, it was additionally necessary Accepted for publication March 15, 1978. to investigate whether elevated levels of urinary cholesterol were present in all clinical stages of BPH or only in patients with BPH and residual urine with indication for surgical treatment. MATERIALS A N D METHODSNonesterified and total urinary cholesterol were analyzed in 2 ml aliquots of 24-hour urine. After extraction with 6 ml ethyl acetate for 30 minutes in a rotating system, the urinary phase was removed. The ethyl acetate extract was purified with alkali (2 ml 0 . 1~ NaOH) and water washings and dried under a stream of nitrogen. The residue was dissolved with 0.1 ml of the internal standard solution (10 mg 4-androstene-3, 17-dione/dl isooctane), followed by GLC on a 180 cm 1 % XE 60 column, i.d. 2 mm, temp. const. 220 C. Quantitation was performed due to the peak height ratio, since alterations of cholesterol and 4-androstenedione concentration gave a linear response.

Correlation of total cholesterol and protein in urine in patients with the NEPHROTIC syndrome

“…220 ° C. Quantitation was performed due to the peak height ratio, since alterations of cholesterol and 4-androstenedione concentrations gave a linear response. For the determination of total urinary cholesterol hydrolysis of the dried extract with 0.1 ml 3 N KOH and 0.9 ml ethanol for 60 min at 60°C was necessary prior to GLC [4]. The specificity of the method was confirmed by simultaneous mass-fragment•metric analysis in selected samples +.…”

The Human Genome as Common Heritage: Common Sense or Legal Nonsense?

“…The human genome is also an abstract concept in that no particular, singular, natural object is the referent of the phrase "the human genome." 56 Parents do not pass on just one single human genome to their children. Rather, each person receives a unique genome (unless she is a monozygotic twin) created by the combination of chromosomes inherited from each parent.…”

“…Furthermore, "if the human genome is an abstraction and not a natural object, people's worries about preserving its integ-rity for future generations become concerns about the future of an idea, not a natural resource." 59 Although the human genome is an abstraction, one might understand this abstraction as being embodied in the genomes of all of the people who exist and all who will exist. One might think of the human genome as a kind of cumulative record, in which each piece plays an important role in creating the whole, and together they represent something more significant than merely the sum of their parts.…”

Η Συμβολη Και Η Σημασια Της Διορθικης Υπερηχογραφιας Στην Σταδιοποιηση Του Καρκινου Του Προστατη

“…χοληστερόλη των ούρων η οποία αυξάνει σε ποσοστό 52,3% περίπου σε ασθενείς με καρκίνο προστάτη σταδίου Α και Β και 63,2% σε ασθενείς σταδίου D και C(83,84,85).…”