Uridine treatment prevents REM sleep deprivation-induced learning and memory impairment

Öcalan, Büşra; Çakır, Ayşen; Koçoğlu, Sema Serter; Suyen, Guldal Gulec; Kahveci, Nevzat

doi:10.1016/j.neures.2019.01.003

Cited by 19 publications

(8 citation statements)

References 52 publications

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“…Sleep deprivation (SD) refers to the inability to achieve adequate undisturbed night sleeps because of environmental or personal reasons. In humans, SD is associated with several adverse effects, including impaired learning and memory, physiology, psychology, and immune functions [1,2]. Sleep reportedly has an antioxidative function [3], and previous studies revealed that SD alters systemic and brain energy metabolism [4,5], possibly because of an accumulation of reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR Signaling Pathway in the Liver of Sleep-Deprived Rats

Zhang

et al. 2020

Oxidative Medicine and Cellular Longevity

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Sleep deprivation adversely affects the digestive system. Multiple studies have suggested sleep deprivation and oxidative stress are closely related. Autophagy can be triggered by oxidative stress as a self-defense strategy to promote survival. In this study, we investigated the effects of sleep deprivation on liver functions, oxidative stress, and concomitant hepatocyte autophagy, as well as the associated pathways. Enzymatic and nonenzymatic biochemical markers in the serum were used to assess hepatic function and damage. To evaluate the occurrence of autophagy, expression of autophagy-related proteins was tested and autophagosomes were labeled. Additionally, methane dicarboxylic aldehyde (MDA), antioxidant enzymes, and the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway were analyzed using chemical methods and a Western blot. Serum alanine transaminase, aspartate aminotransferase, and alkaline phosphatase increased in sleep-deprived rats. Total protein and albumin abundance was also abnormal. Sleep deprivation induced histopathological changes in the liver. The superoxide dismutase level decreased significantly in the liver of sleep-deprived rats. In contrast, the MDA content increased in the sleep deprivation group. Moreover, the microtubule-associated protein 1 light chain 3 beta (LC3B) II/I ratio and Beclin I content increased considerably in the sleep-deprived rats, while p62 levels decreased. Sleep deprivation apparently inhibited the AKT/mTOR signaling pathway. We conclude that sleep deprivation can induce oxidative stress and ultimately cause liver injury. Autophagy triggered by oxidative stress appears to be mediated by the AKT/mTOR pathway and plays a role in relieving oxidative stress caused by sleep deprivation.

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Section: Introductionmentioning

confidence: 99%

Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR Signaling Pathway in the Liver of Sleep-Deprived Rats

Zhang

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…In addition, distribution of the CREB protein from the whole brain was reduced to only the brain cell layer. To the best of our knowledge, the brain‐stained CREB protein result represents the first molecule that has shown correlation between cognition and overexpression or reduced expression of CREB, which may facilitate or inhibit cognitive behavior 56,57 …”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, the brainstained CREB protein result represents the first molecule that has shown correlation between cognition and overexpression or reduced expression of CREB, which may facilitate or inhibit cognitive behavior. 56,57 PKA/ERK/CREB signaling has been shown to be associated with cognitive behavior in cultured hippocampal neurons. 58 In this study, azadirachtin indirectly affected the phosphorylation of PKA protein.…”

Section: Discussionmentioning

confidence: 99%

Azadirachtin downregulates the expression of the CREB gene and protein in the brain and directly or indirectly affects the cognitive behavior of the Spodoptera litura fourth‐instar larvae

Qin

You

Zhang

et al. 2021

Pest Management Science

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BACKGROUND Azadirachtin has the potential to be used for pest control. Nevertheless, few studies have investigated the effects of azadirachtin on the cognitive behavior of pests. In this study, expression of the cAMP response element‐binding protein (CREB) and its gene were studied via a series of experiments in Spodoptera litura larvae treated with azadirachtin. RESULTS RNA‐Seq analysis of S. litura larvae treated with azadirachtin was undertaken. According to Kyoto Encyclopedia of Genes and Genomes analysis, the top 20 enriched pathways included neuroactive ligand–receptor interaction pathways, with seven significantly differentially expressed genes including CREB. Quantitative real time polymerase chain reaction (qRT‐PCR) results indicated that the CREB gene was expressed during all developmental stages of S. litura, but relative expression of the CREB gene was significantly downregulated after treatment with azadirachtin. Grayscale statistical analysis also showed that expression levels of protein kinase A (PKA), extracellular signal‐regulated kinase (ERK) and CREB proteins were significantly downregulated after treatment with azadirachtin. Moreover, RNA interference results showed that the effect of azadirachtin on the cognitive behavior of S. litura was consistent with that seen after interfering with CREB. In addition, larval selectivity to addictive odor sources was reduced, and the initial reaction time was increased. CONCLUSIONS This study clarified that azadirachtin can affect the cognitive behavior of S. litura and treatment with azadirachtin resulted in a downregulation of gene and protein expression of CREB and its pathway proteins. © 2020 Society of Chemical Industry

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“…Previous research has also shown that TSD decreases working memory capacity (Peng et al, 2020[ 84 ]). RSD impairs spatial memory performance in rats (Ocalan et al, 2019[ 79 ]). 72 hours RSD induces an impairment effect on spatial learning and memory of rats in the Morris water maze apparatus (Wang et al, 2009[ 117 ]).…”

Section: Sd and Memorymentioning

confidence: 99%

Inconsistent effects of sleep deprivation on memory function

Vaseghi

Arjmandi-Rad

Kholghi

et al. 2021

EXCLI Journal; 20:Doc1011; ISSN 1611-2156

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Uridine treatment prevents REM sleep deprivation-induced learning and memory impairment

Cited by 19 publications

References 52 publications

Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR Signaling Pathway in the Liver of Sleep-Deprived Rats

Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR Signaling Pathway in the Liver of Sleep-Deprived Rats

Azadirachtin downregulates the expression of the CREB gene and protein in the brain and directly or indirectly affects the cognitive behavior of the Spodoptera litura fourth‐instar larvae

Inconsistent effects of sleep deprivation on memory function

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