Uricase grafted nanoconducting matrix based electrochemical biosensor for ultrafast uric acid detection in human serum samples

Verma, Shilpi; Choudhary, Jyoti; Singh, Krishna Pal; Chandra, Pranjal; Singh, Surinder P.

doi:10.1016/j.ijbiomac.2019.02.121

Cited by 87 publications

(32 citation statements)

References 44 publications

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“…The sensor required lower positive potential (low overpotential) compared to traditional electrochemical sensors. The linear range was from 50 to 800 µM with good selectivity and real sample analysis results [183,185]. These works show the advantage of using ECBs compared to traditional methods in the detection of small biomolecules.…”

Section: Mnps In Small Biomolecule Sensingmentioning

confidence: 72%

“…The low LOD of 0.066 µM and wide linear range of 50 to 700 µM shows the effectiveness of using UOx enzyme in the modification process [182,184]. In another work, an AuNP and rGO complex was utilized for the immobilization of UOx [183,185]. This biosensor was utilized for the rapid detection of UA from human serum samples.…”

Section: Mnps In Small Biomolecule Sensingmentioning

confidence: 99%

“…Carcinogenesis happens at a genetic level in the cell, and follows a complex pathway that ultimately disturbs the homeostatic equilibrium by altering the cell death and cell proliferation rate [185]. Despite tremendous efforts and development, treatment of cancer is still challenging because of the following reasons: (i) proliferation of cancer cells and cell proteins through mutation of the proto-oncogenes; (ii) rejection of growth inhibition signals; (iii) evasion of apoptosis or activating anti-apoptotic genes in cells [69,190,191].…”

Section: Mnps In Cancer Biomarker Detectionmentioning

confidence: 99%

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Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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With the rise in public health awareness, research on point-of-care testing (POCT) has significantly advanced. Electrochemical biosensors (ECBs) are one of the most promising candidates for the future of POCT due to their quick and accurate response, ease of operation, and cost effectiveness. This review focuses on the use of metal nanoparticles (MNPs) for fabricating ECBs that has a potential to be used for POCT. The field has expanded remarkably from its initial enzymatic and immunosensor-based setups. This review provides a concise categorization of the ECBs to allow for a better understanding of the development process. The influence of structural aspects of MNPs in biocompatibility and effective sensor design has been explored. The advances in MNP-based ECBs for the detection of some of the most prominent cancer biomarkers (carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), Herceptin-2 (HER2), etc.) and small biomolecules (glucose, dopamine, hydrogen peroxide, etc.) have been discussed in detail. Additionally, the novel coronavirus (2019-nCoV) ECBs have been briefly discussed. Beyond that, the limitations and challenges that ECBs face in clinical applications are examined and possible pathways for overcoming these limitations are discussed.

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Section: Mnps In Small Biomolecule Sensingmentioning

confidence: 72%

Section: Mnps In Small Biomolecule Sensingmentioning

confidence: 99%

Section: Mnps In Cancer Biomarker Detectionmentioning

confidence: 99%

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Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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“…The % recovery value for each spiked concentration was calculated as per the previous literatures. 33,34 The % recovery values for the spiked samples were 93. .06% suggesting the reliability of the existing biosensor and its efficiency towards UA analysis in clinical detection (Table 1).…”

Section: Detection Of Ua Using Uox/fc/cu 2 O/gce Electrodementioning

confidence: 99%

A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode

Yan

Yang

et al. 2020

Sci Rep

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A uric acid (UA) electrochemical biosensor was constructed using ferrocene (fc) decorated cuprous oxide (cu 2 o) enhanced electro-active characteristics and covalently immobilized with uricase (Uox) on glassy carbon electrode (Gce). the electrochemical characteristics of the fabricated electrode was analysed by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry (DpV). DpV studies revealed rapid response of fabricated electrode Uox/fc/cu 2 o/Gce towards UA in a wide concentration range of 0.1-1,000 μM with a sensitivity of 1.900 μA mM −1 cm −2 and very low detection limit of 0.0596 μM. A very low magnitude Michaelis-Menten constant (Km) value was evaluated as 34.7351 μM which indicated the chemical attraction of the enzyme towards the UA was much higher. the developed biosensor was successfully applied to detect UA in human urine samples. Moreover, reproducibility and stability studies demonstrated the fabricated UOx/Fc/ cu 2 O/GCE biosensor had high reproducibility with a RSD of 2.8% and good reusability with a RSD of 3.2%. Specificity studies results showed the fabricated biosensor had strong anti-interference ability. the improved sensor performance was attributed to the synergistic electronic properties of cu 2 o and fc that provided enhances delectrocatalytic activity and electron transfer. the present biosensor can be extended for use in clinical settings.

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“…According to the characterization results, the improved electrode had an eminent electrochemical reduction peak. In the same year, Verma et al ( 2019 ) used gold nanoparticles to decorate graphene oxide nanocomposite thin films, which were covalently immobilized with uricase enzyme to detect UA sensitively and selectively. The interference in the real samples was evaluated, and the mixed samples were measured by UA.…”

Section: Application Of Carbon Nanomaterials In Metabolic Disease Detmentioning

confidence: 99%

Electrochemical Sensors Based on Carbon Nanomaterial Used in Diagnosing Metabolic Disease

Zhang

2020

Front. Chem.

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Metabolic diseases have become common diseases with the improvement of living standards because of changed dietary habits and living habits, which seriously affect health. Currently, related biomarkers have been widely used as important indicators for clinical diagnosis, treatment, and prognosis of metabolic diseases. Among all detection methods for biomarkers of metabolic diseases, electrochemical sensor technology has the advantages of simplicity, real-time analysis, and low cost. Carbon nanomaterials were preeminent materials for fabricating electrochemical sensors in order to enhance the performance. In this paper, we summarize the research progress in the past 3 years of electrochemical sensors based on carbon nanomaterials in detecting markers of metabolic diseases, which include carbon nanotubes, graphene, carbon quantum dots, fullerene, and carbon nitride. Additionally, we discuss the future prospects for this field.

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Uricase grafted nanoconducting matrix based electrochemical biosensor for ultrafast uric acid detection in human serum samples

Cited by 87 publications

References 44 publications

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode

Electrochemical Sensors Based on Carbon Nanomaterial Used in Diagnosing Metabolic Disease

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