A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode

Yan, Qinghua; Na, Zhi; Yang, Li; Xu, Guangri; Feng, Qigao; Zhang, Qiqing; Sun, Song

doi:10.1038/s41598-020-67394-8

Cited by 94 publications

(47 citation statements)

References 37 publications

(35 reference statements)

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“…The obtained value of sensitivity is much higher than the ones reported in literature (3 to 40 (µA)mM −1 ) for other uric acid biosensors [23,24]. Ya et al [25] has recently reported a uric acid biosensor based on Cu 2 O nanoparticles on glassy carbon electrode and further decorated with ferrocene and reported a sensitivity of 1.9 µA/(mM-cm 2 ). Kudo et al [26] reported a uric acid biosensor based on the measurement of H 2 O 2 produced in the enzymatic reaction and obtained a sensitivity of 170 nA/mM in saliva.…”

Section: Sensing Of Uric Acidmentioning

confidence: 59%

Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Jindal

Gupta

Tomar

2021

Springer Proceedings in Materials

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A novel uric acid biosensor employing ZnO thin film as matrix is developed using pulsed laser deposition technique. The dependence of electrocatalytic properties of ZnO thin films on the ZnO processing pressure during growth is studied. It has been observed that the growth kinetics of ZnO matrix play a critical role in governing the electron transfer characteristics of ZnO thin film based biosensors. It is found from the cyclic voltammetric measurements that the peak oxidation current of ZnO/ITO/glass electrode increases with a rise in pressure of ambient gas from 1 to 100 mT and is maximum (548 µA) for ZnO thin film based electrode prepared in an oxygen ambient of 100 mT. The variation in peak current with change in processing pressure is attributed to the change in surface properties, which largely depends on the mean free path and kinetic energy of ablated species arriving at the substrate. The optimized ZnO thin film (100 mT) offers high surface coverage (9.74 × 10 -9 mol/cm 2 ) during immobilization of uric acid resulting in a sensitivity of 122 µA/(mM-cm 2 ). In addition, the prepared ZnO based biosensor exhibit high affinity towards detection of uric acid (Km~0.07 mM), low limit of detection (0.01 mM) along a storage stability of more than 20 weeks. Thus, the present work suggests an important role of plume kinetics for the fabrication of ZnO thin film based biosensors.

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Section: Sensing Of Uric Acidmentioning

confidence: 59%

Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Jindal

Gupta

Tomar

2021

Springer Proceedings in Materials

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“…So, we can assume that our suggested procedure can be applied for the indirect quantification of MEB. Well-dispersed Cu 2 ONPs-C over graphite grain surfaces was beneficial for the electrocatalytic reaction that may occur at the electrode surface [18]. The markable enhancement of Cu + oxidation current indicates the electrocatalytic activity of the Cu 2 ONPs-C@G, which can be attributed to synergistic electrochemical activity between Cu 2 ONPs-C and graphite grain surfaces, which in turn can be illustrated as the existence of the Cu + /Cu 2+ redox couple, making the electrode surface highly sensitive.…”

Section: Electrochemical Behavior Of Meb On the Cu 2 Onps-c@g/pementioning

confidence: 99%

“…Because of these properties, metal and metal oxide nanoparticles have been used successfully for increasing carbon paste electrode sensitivity toward various electroanalytical applications [15,16]. It was found in the literature that pure Cu2ONPs [11] and Cu2ONPs supported on carbon materials were applied successfully in different voltametric determinations [17,18].…”

Section: Introductionmentioning

confidence: 99%

Graphite Studded with Facile-Synthesized Cu2O Nanoparticle-Based Cubes as a Novel Electrochemical Sensor for Highly Sensitive Voltametric Determination of Mebeverine Hydrochloride

et al. 2021

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Herein, a feasible chemical reduction method followed by intensive mixing was applied for the preparation of an attractive material based on graphite studded with cuprous oxide nanoparticle-based cubes (Cu2ONPs–C@G). Transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray diffraction (XRD) and cyclic voltammetry (CV) were utilized for characterization. Cuprous oxide nanoparticles (Cu2ONPs), with a diameter range mainly distributed from 4 to 20 nm, aggregate to form microcubes (Cu2ONPs–C) with an average diameter of about 367 nm. Paste electrode was prepared using Cu2ONPs–C@G (Cu2ONPs–C@G/PE) for voltametric quantification of the musculotropic antispasmodic drug: mebeverine hydrochloride (MEB). The electrochemical behavior of MEB was studied using CV, and the optimum analytical parameters were investigated using square wave adsorptive anodic stripping voltammetry (SWAdASV). Moreover, density functional theory (DFT) was used to emphasize the ability of MEB to form a complex with Cu2+, confirming the suggested electrochemical behavior of MEB at Cu2ONPs–C@G/PE. With good stability and high reproducibility, SWAdASV of Cu2ONPs–C@G/PE shows successful quantification of MEB over the concentration range of 5.00 × 10−11–1.10 × 10−9 M with lower limit of detection (LOD) and lower limit of quantification (LOQ) values of 2.41 × 10−11 M and 8.05 × 10−11 M, respectively. Finally, accurate quantification of MEB in dosage forms (tablets) and biological fluids (spiked human urine and plasma samples) was achieved using Cu2ONPs-C@G/PE.

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“…Intercalation of propoxur with layered zinc hydroxide produces a nanocomposite material that has good electron transfer ability and a large surface-tovolume ratio. It has also been reported that this material shows low toxicity, high thermal stability, biocompatibility and the potential for controlled release [26,27]. Based on the listed advantages, in this work, we are presenting for the first time LZH-SDS-PRO material as a mediator used to increase the electrocatalytic activity of the redox reaction important for the determination of UA.…”

Section: Introductionmentioning

confidence: 99%

Sensitive determination of uric acid at zinc layered hydroxide-sodium dodecyl sulphate-propoxur nanocomposites

Tajudin¹,

Ahmad

Isa

et al. 2022

J. Electrochem. Sci. Eng.

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An electrochemical chemical sensor for determination of uric acid (UA) with highly sensitive and widely working range was fabricated using zinc layered hydroxide-sodium dodecyl sulphate-propoxur (ZLH-SDS-PRO) nanocomposites modified with multiwall carbon nanotubes (MWCNT). Introduction of the ZLH-SDS-PRO as a conducting matrix has enhanced the conductivity of MWCNT. The ZLH-SDS-PRO/MWCNT morphology was characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), meanwhile the electrochemical behaviour of UA and K3[Fe(CN)6] at ZLH-SDS-PRO/MWCNT paste electrode was studied by square wave voltammetry (SWV) and cyclic voltammetry (CV), respectively. Under the optimized experimental conditions, the electrode established linear plot for UA from 7.0 µmol/L to 0.7 mmol/L (R2 = 0.9920) and the LOD was calculated to be 4.28 µmol/L (S/N = 3). The fabricated electrochemical electrode was successfully applied to urine samples and exhibit excellent stability and reproducibility, which made it worthwhile to be explored for analytical applications.

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A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode

Cited by 94 publications

References 37 publications

Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Electrocatalytic Properties of ZnO Thin Film Based Biosensor for Detection of Uric Acid

Graphite Studded with Facile-Synthesized Cu2O Nanoparticle-Based Cubes as a Novel Electrochemical Sensor for Highly Sensitive Voltametric Determination of Mebeverine Hydrochloride

Sensitive determination of uric acid at zinc layered hydroxide-sodium dodecyl sulphate-propoxur nanocomposites

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