− A phytochemical study of Alphonsea cylindrica King (unreported) has led to the isolation of six alkaloids. The compounds were identified as kinabaline (1; azafluorenone alkaloid), muniranine (2), O-methylmoschatoline (3; oxoaporphine alkaloid), lysicamine (4), atherospermidine (5) and N-methylouregidione (6; 4, 5-dioxoaporphine alkaloid). The structures of the isolated compounds were determined based on the spectroscopic techniques and by comparison with data reported in the literature. Alkaloid 2 was isolated as a new derivative of azafluorenone while alkaloids 1, 3 -6 were isolated for the first time from Alphonsea species. In addition, alkaloid 3 and 4 showed inhibition zone against Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus cereus in disc diffusion test. The minimum inhibition concentration (MIC) values of lysicamine (4) against S. aureus, B. cereus and P. aeruginosa were found to be smaller than O-methylmoschatoline (3). Therefore, the reported antibacterial activity showed the potential of this plant as natural antibacterial agent and supported the documented traditional use of Alphonsea sp. in the treatment of diarrhea and fever.

In this study, hydroxyapatite (HAp) was extracted from freshwater fish scales and saltwater fish scales using thermal decomposition method at various temperatures. The percentages of HAp in each species of raw fish scales were analysed using thermogravimetry analysis (TGA) whereas Fourier transform infrared (FTIR) was used to confirm the present of HA. The crystallinity of the HAp was studied using x-ray diffractometer (XRD). TGA and FTIR are found to be useful techniques to predict the composition of the HAp present in the fish scales. The results show that the composition of HAp in the fish scales was not significantly dependent on the species of the fish. The percentage of HAp in the fish scales ranged from 40 to 45 wt%. The heating temperature had an effect on the crystallinity and colour of the HAp produced. Results from FTIR and XRD confirmed that the HAp extracted from fish scales was similar to the HAp standard. However, the natural HAp produced from saltwater fish scales was more crystalline than that produced from freshwater fish scales. The HAp produced from this study is relatively cheap and more importantly they are HALAL for four billion Muslims population around the world.

The behaviour of fatigue growth and cyclic tip deformation of long cracks due to two successive single overloads was investigated both experimentally and numerically. The results show the effect of the ratio of the second and first overloads, and the crack increment between the two overloads. The contributions of both crack tip blunting and residual stress fields were separated and accommodated in a previously developed crack tip deformation parameter, which was utilized to predict the resulting fatigue crack growth behaviour. The following trends were experimentally observed. Should the ratio of the second and first overloads not be less than one, fatigue crack growth rates followed the predictions based on the second overload. Otherwise, either of the following two situations resulted: (1) when the two overloads were closely applied, the second overload caused an initial acceleration in growth rates followed by a behaviour controlled by the first overload; (2) when the second overload was applied after the crack growth had reached its minimum rate due to the first overload, more retardation in growth rate was observed. Based on the model developed in the paper, it is possible to enhance the retardation effect of an overload if this overload is preceded by another overload. This enhancement depends on the ratio of the two overloads and the crack increment between them.

A two‐dimensional elastic–plastic finite element analysis was utilized to investigate the transition behaviour of a physically short fatigue crack following the application of a single overload cycle. The deformation accommodated at the tip of a crack artificially advancing with a fully reversed load was considered. The development of the cyclic crack tip opening displacement was computed and then modelled to include the effects of the stress level of the base cycles, overload pattern and crack length at which the transient cycle was applied. The cyclic crack tip opening displacement was initially of a relatively high value. It decreased and then increased to match the behaviour under the base load cycles. The extent and location of both the minimum and matching points were dependent on the overload crack length and the stress compared with the material’s yield stress. In the case of the yield stress being exceeded by the overload, the minimum and the‐return‐to‐normality points are identical. A previously developed crack tip deformation parameter was invoked to predict relevant experimental fatigue growth rates of short cracks reported in the literature.

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