Uric acid and creatinine biosensors with enhanced room-temperature storage stability by a multilayer enzyme matrix

Liu, Yiqun; Luo, Xian; Dong, Yaping; Hui, Miao; Xu, Lingyi; Li, Haixia; Lv, Jicheng; Yang, Li; Cui, Yue

doi:10.1016/j.aca.2022.340264

Cited by 10 publications

(3 citation statements)

References 44 publications

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“…This indicates that the enzyme has good storage stability. Previous studies have shown that low storage stability limits the practical application of enzymes [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase

Zhai

Duan

et al. 2022

IJERPH

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Sulfide is a toxic pollutant in the farming environment. Microbial removal of sulfide always faces various biochemical challenges, and the application of enzymes for agricultural environmental remediation has promising prospects. In this study, a strain of Cellulosimicrobium sp. was isolated: numbered strain L1. Strain L1 can transform S2−, extracellular enzymes play a major role in this process. Next, the extracellular enzyme was purified, and the molecular weight of the purified sulfur convertase was about 70 kDa. The sulfur convertase is an oxidase with thermal and storage stability, and the inhibitor and organic solvent have little effect on its activity. In livestock wastewater, the sulfur convertase can completely remove S2−. In summary, this study developed a sulfur convertase and provides a basis for the application in environmental remediation.

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“…This indicates that the enzyme has good storage stability. Previous studies have shown that low storage stability limits the practical application of enzymes [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase

Zhai

Duan

et al. 2022

IJERPH

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show abstract

“…Biosensors are generally based on bioreceptors such as enzymes, DNA, etc. for the identification of molecular analytes and/or microorganisms [16][17][18][19][20]. Enzymes are catalytic proteins used to enhance the rate of a reaction without being consumed or changing the balance of chemical reactions.…”

Section: Enzymatic Receptorsmentioning

confidence: 99%

Electrochemical Creatinine (Bio)Sensors for Point-of-Care Diagnosis of Renal Malfunction and Chronic Kidney Disorders

et al. 2023

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In the post-pandemic era, point-of-care (POC) diagnosis of diseases is an important research frontier. Modern portable electrochemical (bio)sensors enable the design of POC diagnostics for the identification of diseases and regular healthcare monitoring. Herein, we present a critical review of the electrochemical creatinine (bio)sensors. These sensors either make use of biological receptors such as enzymes or employ synthetic responsive materials, which provide a sensitive interface for creatinine-specific interactions. The characteristics of different receptors and electrochemical devices are discussed, along with their limitations. The major challenges in the development of affordable and deliverable creatinine diagnostics and the drawbacks of enzymatic and enzymeless electrochemical biosensors are elaborated, especially considering their analytical performance parameters. These revolutionary devices have potential biomedical applications ranging from early POC diagnosis of chronic kidney disease (CKD) and other kidney-related illnesses to routine monitoring of creatinine in elderly and at-risk humans.

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“…[1] Emerging biosensing technologies have been reported to address this urgent need to create POC diagnostics. There have been picric acid methods for the detection of creatinine, [10,11] Nibased biosensors for the electrocatalytic detection of urea, [12,13] and immunoturbidimetry assays for the estimation of HSA. [8,14] However, these novel technologies come with a number of limitations, and some of their sensing characteristics limit any potential practical application.…”

Section: Introductionmentioning

confidence: 99%

A Point‐of‐Care Sensing Platform for Multiplexed Detection of Chronic Kidney Disease Biomarkers Using Molecularly Imprinted Polymers

Li,

Luo,

Kong

et al. 2024

Adv Funct Materials

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Chronic kidney disease (CKD) is one of the most serious non‐communicable diseases affecting the population. In the early‐stages patients have no obvious symptoms, until it becomes life‐threatening leading end‐stage kidney failure. Therefore, it is important to early diagnose CKD to allow therapeutic interventions and progression monitoring. Here, a point‐of‐care (POC) sensing platform is reported for the simultaneous detection of three CKD biomarkers, namely creatinine, urea, and human serum albumin (HSA), using reduced graphene oxide/polydopamine‐molecularly imprinted polymer (rGO/PDA‐MIP) fabricated with novel surface‐molecularly imprinting technology. A multi‐channel electrochemical POC readout system with differential pulse voltammetry (DPV) function is developed, allowing the simultaneous detection of the three biomarkers, in combination with the surface‐MIP electrodes. This sensing platform achieves the record low limit‐of‐detection (LoD) at a femtomolar level for all three analytes, with wide detection ranges covering their physiological concentrations. Clinical validation is performed by measuring these analytes in serum and urine from healthy controls and patients with CKD. The average recovery rate is 81.8–119.1% compared to the results obtained from the hospital, while this platform is more cost‐effective, user‐friendly, and requires less sample‐to‐result time, showing the potential to be deployed in resource‐limited settings for the early diagnosis and tracking progression of CKD.

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Uric acid and creatinine biosensors with enhanced room-temperature storage stability by a multilayer enzyme matrix

Cited by 10 publications

References 44 publications

Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase

Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase

Electrochemical Creatinine (Bio)Sensors for Point-of-Care Diagnosis of Renal Malfunction and Chronic Kidney Disorders

A Point‐of‐Care Sensing Platform for Multiplexed Detection of Chronic Kidney Disease Biomarkers Using Molecularly Imprinted Polymers

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