Urethral striated muscle and extracellular matrix morphological characteristics among mildly diabetic pregnant rats: translational approach

Pículo, Fernanda; Marini, Gabriela; Barbosa, Angélica Mércia Pascon; Damasceno, Débora Cristina; Matheus, Selma Maria Michelin; Felisbino, Sérgio Luís; Daneshgari, Firouz; Rudge, Marilza Vieira Cunha

doi:10.1007/s00192-013-2218-4

Cited by 33 publications

(44 citation statements)

References 27 publications

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“…The STZ model generally represents the etiology of type 1 diabetes or advanced stages of type 2 diabetes (Bell Jr. and Hye, 1983). The STZ model has been extensively studied and used to assess the complications of type 1 diabetes (Badalzadeh et al, 2015;Piculo et al, 2014), learning and memory (Bellush and Rowland, 1989;Flood et al, 1990), natural hedonic processing and drug reward (Carr, 1994;Carr et al, 2000;Galici et al, 2003b;O'Dell et al, 2014). Thus, the STZ model represents a common method of inducing diabetes via disruptions in insulin signaling.…”

Section: Rodent Models Of Diabetes Used To Study Nicotine Rewardmentioning

confidence: 98%

Enhanced vulnerability to tobacco use in persons with diabetes: A behavioral and neurobiological framework

O’Dell

Nazarian

2016

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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Section: Rodent Models Of Diabetes Used To Study Nicotine Rewardmentioning

confidence: 98%

Enhanced vulnerability to tobacco use in persons with diabetes: A behavioral and neurobiological framework

O’Dell

Nazarian

2016

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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“…At term pregnancy, the morphological analysis of the extracellular matrix and urethral striated muscle from urethrovaginal tissues showed that the urethral layers were thin, disorganized, and atrophic and there were more fibrosis replacement and collagen fiber deposition associated to muscle atrophy. In addition to structural analyses, one of the most significant findings was the colocalization of fast and slow fibers and a steady decrease in the proportion of fast to slow fibers by immunohistochemical examination in the mild diabetic pregnant rats [64]. Diabetes is related to accumulation of reactive oxygen species, and tissue ischemia which can interactively or independently contribute to the myopathy causes of skeletal muscle dysfunctions [65, 66].…”

Section: Models Of Mild Diabetes Induction and Repercussions On Thmentioning

confidence: 99%

“…Glycogen granules, lipid droplets, and numerous mitochondria were apparent in the striated muscle cells [64]. Lipid droplets are not membrane bound, and their number and size may vary considerably among different muscle types.…”

Section: Models Of Mild Diabetes Induction and Repercussions On Thmentioning

confidence: 99%

Mild Diabetes Models and Their Maternal-Fetal Repercussions

Damasceno

Sinzato

Bueno

et al. 2013

Journal of Diabetes Research

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The presence of diabetes in pregnancy leads to hormonal and metabolic changes making inappropriate intrauterine environment, favoring the onset of maternal and fetal complications. Human studies that explore mechanisms responsible for changes caused by diabetes are limited not only for ethical reasons but also by the many uncontrollable variables. Thus, there is a need to develop appropriate experimental models. The diabetes induced in laboratory animals can be performed by different methods depending on dose, route of administration, and the strain and age of animal used. Many of these studies are carried out in neonatal period or during pregnancy, but the results presented are controversial. So this paper, addresses the review about the different models of mild diabetes induction using streptozotocin in pregnant rats and their repercussions on the maternal and fetal organisms to propose an adequate model for each approached issue.

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“…Os estudos dessa associação são escassos na literatura. Nosso grupo de pesquisa iniciou a pesquisa translacional em ratas prenhes com diabetes de intensidade grave e moderada com Marini et al 5 e Piculo et al 6 , que evidenciaram que o diabetes e a prenhez em ratas danificam o músculo estriado esquelético e a matriz extracelular uretral.…”

Section: Introductionunclassified

“…A análise imunoistoquímica evidenciou perda da localização anatômica normal das fibras musculares com aumento na proporção de fibras oxidativas 5 . No segundo trabalho, com o intuito de mimetizar os efeitos do diabetes com elevação moderada da glicemia (glicemia entre 120 e 300 mg/dL), verificamos que, além das alterações musculares, podem ser observadas modificações significativas na matriz extracelular, como fibrose, e na ultraestrutura com acúmulo de mitocôndrias, aumento do número de gotas de lipídios e acúmulo de grânulos de glicogênio no grupo diabético prenhe 6 . As alterações encontradas nos dois trabalhos do nosso grupo de pesquisa podem explicar a alta prevalência de IU e disfunção do assoalho pélvico em mulheres com diabetes mellitus (DM) gestacional.…”

Section: Introductionunclassified

Alterações da matriz extracelular causadas pelo diabetes: o impacto sobre a continência urinária

Marini¹,

Rinaldi²,

Damasceno³

et al. 2014

Rev. Bras. Ginecol. Obstet.

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ResumoA prevalência de incontinência urinária em gestantes diabéticas é significantemente elevada e persiste por até dois anos após o parto cesárea, podendo ser a sequela mais frequente da hiperglicemia gestacional comparada a outras complicações. Dessa forma, identificar os fatores de risco para o desenvolvimento da incontinência urinária em diabéticas é o principal objetivo na prevenção dessa condição tão comum. Pesquisas recentes apontam que não apenas o músculo uretral mas também a matriz extracelular uretral desempenham papel importante no mecanismo da continência urinária. Os trabalhos do nosso grupo de pesquisa evidenciaram que, em ratas, o diabetes durante a prenhez lesa a matriz extracelular e o músculo estriado uretral, o que pode explicar a alta prevalência de incontinência e disfunção do assoalho pélvico em mulheres com diabetes mellitus gestacional. O diabetes exerce efeito sobre a expressão, organização e alteração dos componentes da matriz extracelular em diversos órgãos, e a remodelação do tecido e a fibrose parecem ser uma consequência direta dele. Assim, a compreensão do impacto de fatores de risco modificáveis, como o diabetes, permitirá que, utilizando estratégias preventivas, reduzamos as taxas de incontinência urinária, bem como os custos de assistência à saúde, e melhoremos a qualidade de vida das mulheres, especialmente na gestação e no pós-parto. AbstractThe prevalence of urinary incontinence in diabetic pregnant women is significantly high two years after cesarean section. Incontinence can be the most common consequence of hyperglycemia compared to other complications. Thus, identifying the risk factors for the development of urinary incontinence in diabetes is the major aim in the prevention of this very common condition. Recent surveys have shown that not only muscle but also the urethral extracellular matrix play an important role in the mechanism of urinary continence. Translational work on rats by our research group showed that diabetes during pregnancy damages the extracellular matrix and urethral striated muscle, a fact that may explain the high prevalence of urinary incontinence and pelvic floor dysfunction in women with gestational diabetes mellitus. Diabetes affects the expression, organization and change in extracellular matrix components in different organs, and tissue remodeling and fibrosis appear to be a direct consequence of it. Therefore, understanding the impact of modifiable risk factors, such as diabetes, which involves using preventive strategies, can reduce the rates of urinary incontinence and the health care costs, and improve the quality of life of women, especially during pregnancy and postpartum.

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Urethral striated muscle and extracellular matrix morphological characteristics among mildly diabetic pregnant rats: translational approach

Abstract: Mild DM and pregnancy can lead to a time-dependent disorder and tissue remodeling in which the urethral striated muscle and ECM has a fundamental function.

Cited by 33 publications

References 27 publications

Enhanced vulnerability to tobacco use in persons with diabetes: A behavioral and neurobiological framework

Enhanced vulnerability to tobacco use in persons with diabetes: A behavioral and neurobiological framework

Mild Diabetes Models and Their Maternal-Fetal Repercussions

Alterações da matriz extracelular causadas pelo diabetes: o impacto sobre a continência urinária

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