Mild Diabetes Models and Their Maternal-Fetal Repercussions

Damasceno, Débora Cristina; Sinzato, Yuri Karen; Bueno, Aline; Netto, A.O.; Dallaqua, Bruna; Gallego, Franciane Quintanilha; Iessi, Isabela Lovizutto; Corvino, Silvana Barroso; Serrano, R.G.; Marini, Gabriela; Pículo, Fernanda; Calderon, Iracema de Mattos Paranhos; Rudge, Marilza Vieira Cunha

doi:10.1155/2013/473575

Cited by 28 publications

(17 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A continuous glycemic stimulus induces the second phase of insulin secretion, which is less intense and more prolonged 14,15 . In our study, it was found before pregnancy that the pancreas was tolerant to glucose stimulus during oral glucose tolerance test, confirmed by adequate insulin secretion due to the blood glucose levels maintenance below 140 mg/dL (minimum reference value for intolerance glucose) and below 200 mg/dL (minimum reference value for Diabetes mellitus) 16 .…”

supporting

confidence: 67%

Physiological and biochemical measurements before, during and after pregnancy of healthy rats

et al. 2015

View full text Add to dashboard Cite

PURPOSE:To analyze the physiological and biochemical measurements before, during and after pregnancy of healthy rats. METHODS:Wistar adult females rats (n=8) were weighed and blood samples were obtained before, during and after pregnancy for biochemical determinations, chow intake, water consumption and milk production were evaluated. At day 10 postpartum, the rats were killed for weighing of organs and adipose tissues. RESULTS:The results showed increase in body weight, serum insulin and ingestion of water and chow. At day 17 pregnancy, presented normal values in the OGTT. At days 7, 14 and 20 of pregnancy, there was increase in triglyceride levels. At term pregnancy, there was weight gain due to fetal growth. In the postpartum period presented reduced blood glucose levels. The glycemic means were reduced during and after pregnancy compared to after pregnancy. The triglyceride concentrations were increased before and during pregnancy in relation to after pregnancy. The total cholesterol levels presented no changes. CONCLUSION:The use of experimental animals is suitable for evaluation of metabolic changes because the profile of answers found in this study was similar to human profile, showing the relevance of translational research to better understand the pathophysiological mechanisms and possible treatment for diseases.

show abstract

supporting

confidence: 67%

Physiological and biochemical measurements before, during and after pregnancy of healthy rats

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Varios autores han reportado signos de regeneración evidentes a las dos semanas de evolución en ratas inoculadas en el mismo día del nacimiento y en el día dos, mientras que las inyectadas en el quinto día mostraban alteraciones en el patrón histológico en ese mismo tiempo. La capacidad de regeneración de las células β del páncreas en ratas Wistar disminuye rápidamente durante la primera semana a partir del nacimiento y es insignificante en los roedores adultos (1,6,11,12), de ahí que el momento de la administración determine el tipo de diabetes que se induce experimentalmente.…”

Section: Discussionunclassified

“…A pesar de que la acción de la estreptozotocina es específica contra las células β, el resto del islote se ve afectado por los posibles mecanismos de regeneración ya demostrados en modelos experimentales similares al empleado en este trabajo. La generación de nuevas células β puede ocurrir a partir de células indiferenciadas (neogénesis), de células ya diferenciadas que se convierten en otro tipo celular (transdiferenciación) y de la replicación de las propias células β remanentes (proliferación) (1,12).…”

Section: Discussionunclassified

Acción de la estreptozotocina en un modelo experimental de inducción neonatal de la diabetes

Bequer¹,

Gómez²,

Molina³

et al. 2016

biomedica

View full text Add to dashboard Cite

Introducción. El estudio de la diabetes mediante modelos experimentales es fundamental para entender los mecanismos fisiopatológicos de la enfermedad. Objetivos. Comparar en ratas Wistar adultas la acción de inducción de la diabetes de la estreptozotocina según el momento y la vía de inoculación del fármaco durante el periodo neonatal mediante la evaluación de variables bioquímicas, metabólicas e histológicas. Materiales y métodos. Se conformaron ocho grupos con ratas Wistar hembras recién nacidas (n=10). Se evaluó la inducción con estreptozotocina (100 mg/kg de peso corporal) según el día (segundo y quinto después del nacimiento) y la vía de inoculación (subcutánea o intraperitoneal). Los controles se inyectaron con solución tampón de citrato sódico. Durante 12 semanas se evaluaron la glucemia, el peso, y la cantidad de alimento y de agua consumida. Se hicieron pruebas de tolerancia a la glucosa oral, se evaluó la hemoglobina ‘glicosilada’, y se hizo el estudio morfométrico e histopatológico del páncreas. Resultados. Casi todos los animales inoculados con estreptozotocina en el quinto día murieron, en tanto que todos los inoculados en el segundo día sobrevivieron. La administración subcutánea de estreptozotocina en el segundo día produjo hiperglucemia, polifagia, polidipsia y disminución de la ganancia de peso corporal, así como alteración de los valores de hemoglobina ‘glicosilada’ y en la prueba de tolerancia a la glucosa. Las lesiones histopatológicas del páncreas, así como la disminución del número de islotes, se observaron con mayor frecuencia con la estreptozotocina aplicada de forma subcutánea en el segundo día, lo cual corroboró que el efecto de este agente inoculado de forma subcutánea causa mayor daño. Conclusiones. La inyección subcutánea de una dosis de 100 mg/kg de estreptozotocina en el segundo día después del nacimiento logró mayor efectividad en la inducción de diabetes moderada en ratas Wistar adultas.

show abstract

“…; Damasceno et al . ). Recently, mild diabetes model was characterized by changes in two or more points in the glucose curve during OGTT (Santos et al .…”

Section: Discussionmentioning

confidence: 97%

Mild diabetes: long‐term effects on gastric motility evaluated in rats

Hauschildt

Corá

Volpato

et al. 2018

Int J Experimental Path

View full text Add to dashboard Cite

Moderate hyperglycaemic levels seem to be related to abnormal gastric motility in diabetes mellitus. However, experimental models designed to evaluate the relationship between motility and diabetes over time are not yet well established. Our objective was to investigate the long-term effects of mild diabetes on gastric motility in rats. Newborn male rats received streptozotocin (mild diabetes groups - MD) or vehicle (control groups - C), and both groups were evaluated after 3 (C3 and MD3) and 6 months (C6 and MD6) postinduction. Mild diabetic animals (MD3 and MD6) showed moderately elevated blood glucose and decreased insulin levels compared with control (C3 and C6). Insulin secretion was enhanced in MD6 compared with MD3, most likely due to partial β-cell regeneration indicated by HOMA-β. In HOMA-IR, it was noticed that MD6 animals had impaired insulin response compared with MD3. Gastric emptying was faster, amplitude of contraction was stronger in MD6 compared with MD3, and in both groups, the differences were significant when compared with control animals. A significant abnormal rhythmic index was calculated for the mild diabetic groups, despite unchanged mean frequency of contraction. In conclusion, despite increased insulin levels over time, constant levels of moderate hyperglycaemia are also related to abnormal gastric motility and impairment of gastric function.

show abstract

Mild Diabetes Models and Their Maternal-Fetal Repercussions

Cited by 28 publications

References 75 publications

Physiological and biochemical measurements before, during and after pregnancy of healthy rats

Physiological and biochemical measurements before, during and after pregnancy of healthy rats

Acción de la estreptozotocina en un modelo experimental de inducción neonatal de la diabetes

Mild diabetes: long‐term effects on gastric motility evaluated in rats

Contact Info

Product

Resources

About