Urethral Replacement Using Cell Seeded Tubularized Collagen Matrices

Filippo, Roger E. De; Yoo, James J.; Atala, Anthony

doi:10.1097/00005392-200210020-00032

Cited by 77 publications

(88 citation statements)

References 21 publications

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“…Scaffolds seeded with cells, particularly when they are preconditioned in a bioreactor prior to implantation, often integrate into tissue in a vascularized, non-fibrotic manner. The scaffold without cells can heal in a fibrotic, avascular manner [15]. Though this is speculation, it may be the presence of cell-secreted ECM components that are transitioning the biomaterial scaffold from a "biocompatible" material generating the classic FBR to a structure that heals in a vascularized, reconstructive manner.…”

Section: The Future Of Biocompatibilitymentioning

confidence: 99%

The Biocompatibility Manifesto: Biocompatibility for the Twenty-first Century

Ratner

2011

J. of Cardiovasc. Trans. Res.

122

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Section: The Future Of Biocompatibilitymentioning

confidence: 99%

The Biocompatibility Manifesto: Biocompatibility for the Twenty-first Century

Ratner

2011

J. of Cardiovasc. Trans. Res.

122

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“…Urethral reconstruction is frequently used in the treatment of both congenital and acquired anomalies (1)(2)(3)(4)(5)(6). When the urethra needs to be repaired or reconstructed, either partially or totally, different surgical approaches can be used such as anastomosis of the urethral stumps, flaps or grafts.…”

Section: Introductionmentioning

confidence: 99%

“…Although such techniques are still largely employed, in several occasions adverse effects such as fistulas, stenosis, infections, stone formation, graft contracture or diverticula formation may appear. In addition to morbidity at the donor site, there is tissue scarcity when such patients undergo a number of surgical interventions (1,2,(4)(5)(6)(7)(8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Integration of collagen matrices into the urethra when implanted as onlay graft

Sayeg¹,

Freitas-Filho²,

Waitzberg

et al. 2013

Int. braz j urol.

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Objective: To assess the integration of decellularized heterologous collagen matrices into the urethra, when implanted with no cells or when seeded with autologous smooth muscle cells. Materials and Methods: Eighteen New Zealand rabbits were randomly assigned to two groups: Group I (n = 9) -animals undergoing urethral segment resection with interposition of a patch of heterologous collagen matrix seeded with autologous smooth muscle cells; Group II (n = 9) -animals undergoing resection of a urethral segment with interposition of a decellularized heterologous collagen matrix patch. Two animals from each group were sacrificed on postoperative days seven, fourteen and twenty-eight; three animals from each group were sacrificed at the end of three postoperative months. At the end of the third month one animal from each group underwent urethroscopy for urethral integrity assessment and one animal from each group had its microcirculation image captured by a SDF device (Side-stream Dark Field -Microscan Analysis Software). One animal from each group in each euthanasia period underwent cystourethrography so as the urethra could be viewed at flow time. The matrices integration was assessed through histological examination using hematoxylin and eosin (H&E), Masson trichrome (MT), Picrosirius red and Von Willebrand staining. In a blind study with two pathologists all the slides were studied. Results: The matrices whether seeded or not with autologous muscle cells were able to restore the architecture of the urethra, but were eliminated from the first week on, before incorporation. Microcirculation of the neourethra, at the end of the third month, showed the same characteristics as a normal urethra in both groups of animals. Conclusion: Natural heterologous matrices implanted in the urethra as onlay graft were not incorporated into its walls but were able to fully restore the cell architecture of the organ, regardless of being seeded or not with autologous muscle cells.

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“…This matrix has been used experimentally and clinically for several applications, including urethra and bladder reconstruction procedures. [7][8][9] In this study, we investigated whether ABM possesses the ideal material characteristics required for penile reconstruction. We examined the applicability and functional outcome of ABM for tunica repair.…”

Section: Introductionmentioning

confidence: 99%

Tunica repair with acellular bladder matrix maintains corporal tissue function

et al. 2007

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Penile conditions, such as Peyronie's disease or tumor resection may require surgical reconstruction of the tunica albuginea. Various materials have been proposed, as a biomaterial for tunica albuginea repair, however, little functional data are available. We examined the applicability and functional outcome of a collagen-based matrix derived from the bladder (acellular bladder matrix (ABM)), as a biomaterial for tunica repair. Biocompatibility testing was performed on the matrix, which included mitochondrial metabolic activity, cell viability and apoptosis. Approximately 50% of the dorsal penile tunica albuginea was replaced with the collagen-based matrix patch after surgical removal in 24 New Zealand White rabbits. Cavernosometry and cavernosography were performed. The animals were killed 1, 2 and 3 months after surgery for analyses. The matrix showed excellent biocompatibility. All animals implanted with the matrix survived without any noticeable untoward effects. There was no evidence of inflammation or infection at the time of retrieval. Cavernosometry of the implanted animals demonstrated normal intracavernosal pressures with visual erections. Cavernosography of the repaired corpora showed a normal anatomical configuration. Biomechanical analysis of the retrieved matrices demonstrated similar tensile strengths as native tunica. Histologically, there was only a minimal inflammatory response, which gradually decreased over time. These results show that ABM is biocompatible, durable and effective when used as a tunica substitute. The matrix may be useful as an off-the-shelf biomaterial for patients requiring tunica albuginea repair.

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Urethral Replacement Using Cell Seeded Tubularized Collagen Matrices

Cited by 77 publications

References 21 publications

The Biocompatibility Manifesto: Biocompatibility for the Twenty-first Century

The Biocompatibility Manifesto: Biocompatibility for the Twenty-first Century

Integration of collagen matrices into the urethra when implanted as onlay graft

Tunica repair with acellular bladder matrix maintains corporal tissue function

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