Urethral catheterization after pharmacological induction for semen collection in dog

Kuczmarski, Antonio Henrique; Barros, Marisol Alves de; Lima, Luiz Felipe Souza de; Motheo, T. F.; Bento, H.J.; Iglesias, G.A.; Sônego, Dábila Araújo; Paz, Regina Celia Rodrigues da

doi:10.1016/j.theriogenology.2020.04.035

Cited by 7 publications

(8 citation statements)

References 22 publications

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“…Etorphine in combination with medetomidine and midazolam was used repeatedly for intensive medical management in a black rhinoceros in captivity ( 11 ) and resulted in reliable immobilization and rapid recovery. The specific α 2 -adrenoceptor agonist medetomidine provides sedation, muscle relaxation and anxiolysis ( 12 ) and, additionally, was found to promote semen emission in other species ( 13 , 14 ). Midazolam, a benzodiazepine receptor agonist, also provides sedation and muscle relaxation ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Cardiopulmonary Parameters and Arterial Blood Gases During Etorphine-Medetomidine-Midazolam Immobilization in Free-Ranging Black Rhinoceroses (Diceros bicornis) Undergoing Electro-Ejaculation—A Preliminary Study

et al. 2021

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Conservation management interventions for the critically endangered black rhinoceros (Diceros bicornis) require immobilization, which offer opportunities for semen collection and cryopreservation to establish genetic reservoirs. In free-ranging rhinoceroses, a combination of the potent opioid etorphine and the tranquilizer azaperone is routinely used for chemical immobilization but is associated with muscle rigidity and severe cardiopulmonary changes. Additionally, azaperone inhibits semen emission. Seven free-ranging, male, sexually mature black rhinoceroses were immobilized with an alternative protocol consisting of 4.5 mg etorphine, 5 mg medetomidine, 50 mg midazolam and 2,500 IU hyaluronidase delivered remotely by darting from a helicopter. During the immobilization, electro-ejaculation was performed with a portable electro-ejaculator, and a species-specific rectal probe. Animals were observed for muscle tremors. Longitudinal changes in respiratory rate, heart rate and peripheral oxyhemoglobin saturation, measured at 5 min intervals, were assessed using a general mixed model. Non-invasive oscillometric blood pressure and arterial blood gas variables were measured at first handling and before reversal and compared using the Wilcoxon rank sum test. All animals were successfully immobilized, showed no muscle tremors, presented with normal heart rates and lactate concentration (<5 mmol/L), recovered uneventfully, but experienced acidemia, hypoxemia and hypercapnia. Induction time and total time in recumbency were 4.2 ± 0.41 and 38.4 ± 6.9 min, respectively. Electro-stimulation commenced after 11.7 ± 3.98 min and completed after 24.3 ± 6.65 min. Semen-rich fractions were successfully collected from six animals. Our observations indicate that etorphine-medetomidine-midazolam provides a promising immobilization protocol for free-ranging black rhinoceroses, that allows for successful electro-ejaculation.

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Section: Introductionmentioning

confidence: 99%

Cardiopulmonary Parameters and Arterial Blood Gases During Etorphine-Medetomidine-Midazolam Immobilization in Free-Ranging Black Rhinoceroses (Diceros bicornis) Undergoing Electro-Ejaculation—A Preliminary Study

et al. 2021

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“…In fact, semen with spermatozoa could not be collected from the Japanese macaques using the UC protocol in felids [ 3 , 24 , 50 ]. It is thought that the following three factors are involved in the regulation of semen collection by UC: adrenergic innervation [ 35 , 47 , 50 ], dose of medetomidine [ 6 ] and the type of drug used for pharmacological stimulation [ 19 , 40 , 47 ]. The release of sperm from the epididymis and/or ductus deferens to the urethra by administration of a α2-adrenergic agonist was reported [ 25 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (<i>Macaca fuscata</i>)

et al. 2022

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“…Other semen collection techniques are urethral catheterization and electro ejaculation. However, this procedure is much more invasive and may lead to urine contamination [70], which in turn may impair semen quality and fertility [71].…”

Section: Semen Collection Proceduresmentioning

confidence: 99%

Diagnostic tests in canine andrology - What do they really tell us about fertility?

et al. 2023

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Urethral catheterization after pharmacological induction for semen collection in dog

Cited by 7 publications

References 22 publications

Cardiopulmonary Parameters and Arterial Blood Gases During Etorphine-Medetomidine-Midazolam Immobilization in Free-Ranging Black Rhinoceroses (Diceros bicornis) Undergoing Electro-Ejaculation—A Preliminary Study

Cardiopulmonary Parameters and Arterial Blood Gases During Etorphine-Medetomidine-Midazolam Immobilization in Free-Ranging Black Rhinoceroses (Diceros bicornis) Undergoing Electro-Ejaculation—A Preliminary Study

Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (<i>Macaca fuscata</i>)

Diagnostic tests in canine andrology - What do they really tell us about fertility?

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