“…These parameters were chosen as commonly used during clinical as well as research settings to estimate sperm fertilizing ability [ 33 ]. While there are in vitro and in vivo models to assess specific aspects of spermatozoa fertilizing ability in other species, we do not have such a capability in the dog [ 16 , 27 , 35 ]. The breeding soundness evaluation guidelines as established by the Society for Theriogenology and the American College of Theriogenologists correlate with the fertilizing ability of stud dogs.…”
Our objective was to determine a clinically relevant range of centrifugation parameters for processing canine semen. We hypothesized that higher gravitational (g) force and longer time of centrifugation would result in improved spermatozoa recovery rate (RR) but poorer semen quality. Cooled storage under standard shipping conditions was used as a stressor to evaluate long-term treatment effects. Individual ejaculates collected from 14 healthy dogs were split into six treatment groups (400 g, 720 g, and 900 g for 5 or 10 min). Sperm RR (%) was calculated post-centrifugation, and plasma membrane integrity (%, Nucleocounter® SP-100™), total and progressive motility (%, subjective and computer-assisted sperm analysis), and morphology (%, eosin-nigrosin staining) were assessed on initial raw semen (T0), post-centrifugation (T1), and 24 h (T2) and 48 h (T3) after cooling. Sperm losses were minimal, and RRs were similar across treatment groups (median >98%, p ≥ 0.062). Spermatozoa membrane integrity was not different between centrifugation groups at any time point (p ≥ 0.38) but declined significantly during cooling (T1 vs. T2/T3, p ≤ 0.001). Similarly, total and progressive motility did not differ across treatments but declined in all groups from T1 to T3 (p ≤ 0.02). In conclusion, our study showed that centrifugation within a range of 400 g–900 g for 5–10 min is appropriate for processing canine semen.
“…These parameters were chosen as commonly used during clinical as well as research settings to estimate sperm fertilizing ability [ 33 ]. While there are in vitro and in vivo models to assess specific aspects of spermatozoa fertilizing ability in other species, we do not have such a capability in the dog [ 16 , 27 , 35 ]. The breeding soundness evaluation guidelines as established by the Society for Theriogenology and the American College of Theriogenologists correlate with the fertilizing ability of stud dogs.…”
Our objective was to determine a clinically relevant range of centrifugation parameters for processing canine semen. We hypothesized that higher gravitational (g) force and longer time of centrifugation would result in improved spermatozoa recovery rate (RR) but poorer semen quality. Cooled storage under standard shipping conditions was used as a stressor to evaluate long-term treatment effects. Individual ejaculates collected from 14 healthy dogs were split into six treatment groups (400 g, 720 g, and 900 g for 5 or 10 min). Sperm RR (%) was calculated post-centrifugation, and plasma membrane integrity (%, Nucleocounter® SP-100™), total and progressive motility (%, subjective and computer-assisted sperm analysis), and morphology (%, eosin-nigrosin staining) were assessed on initial raw semen (T0), post-centrifugation (T1), and 24 h (T2) and 48 h (T3) after cooling. Sperm losses were minimal, and RRs were similar across treatment groups (median >98%, p ≥ 0.062). Spermatozoa membrane integrity was not different between centrifugation groups at any time point (p ≥ 0.38) but declined significantly during cooling (T1 vs. T2/T3, p ≤ 0.001). Similarly, total and progressive motility did not differ across treatments but declined in all groups from T1 to T3 (p ≤ 0.02). In conclusion, our study showed that centrifugation within a range of 400 g–900 g for 5–10 min is appropriate for processing canine semen.
“…° These tests will identify extremely poor or excellent semen, although there are no assurance of fertilizing capability based on a single evaluation. 6 ° The HOST test is positively correlated with normal progressive motility and normal morphology in fresh semen. 7 ° In stallions, the phase contrast technique used for morphologic evaluations resulted in better agreement between evaluators when compared to other morphologic evaluation methods.…”
Section: Importance Of Semen Evaluation At Collectionmentioning
confidence: 94%
“…This is an astute way to maneuver around small volumes from each individual dog collected; however, has the weakness of making it impossible to measure the effect of the experimental parameter on an individual dog. 6 This must be kept in mind when discussing papers moving forward.…”
Section: Comparison (Canine and Equine) And Application Of Semen Proc...mentioning
Advanced reproductive techniques for dogs have become commonplace in general practice during last decade. Most procedures performed in these practices are based on what is known for semen processing and shipping in the equine industry. This presentation reviews current literature of what is known on canine semen processing and attempts to standardize techniques to maximize their successful use in clinical settings.
“…Breeding soundness examination (BSE) in adult male dogs requires serum testosterone (T) assay to assess normality of testicular endocrine function as well as an assessment of prostatic condition whenever infertility is suspected (Arlt et al, 2023; de Souza et al, 2015). When performing a BSE, a single blood sample may be used for the assay of hormones and prostatic serum biomarkers such as canine prostatic‐specific esterase (CPSE).…”
Section: Introductionmentioning
confidence: 99%
“…Breeding soundness examination (BSE) in adult male dogs requires serum testosterone (T) assay to assess normality of testicular endocrine function as well as an assessment of prostatic condition whenever infertility is suspected (Arlt et al, 2023;de Souza et al, 2015).…”
The gonadotropin‐releasing hormone (GnRH) stimulation test is used to investigate testicular production of testosterone (T) when performing a breeding soundness examination. In male dogs with fertility problems, the prostate should also be investigated as prostatic conditions may frequently lower semen quality. Serum concentrations of canine prostatic‐specific esterase (CPSE) increase in dogs with benign prostatic hyperplasia (BPH). When performing a breeding soundness examination in a male dog, GnRH administration is frequently done at the beginning of the process and then both T and CPSE are assayed on the same serum sample collected 1 h following the GnRH injection. The aim of this study was to assess whether or not the administration of GnRH may alter CPSE concentrations in dogs with a healthy prostate. Twenty‐eight client‐owned intact adult male dogs were included in the study. Following a 7‐day sexual rest all male dogs underwent a clinical examination and an ultrasonographic examination of the prostatic gland. Prostatic size and parenchyma of every tested dog were evaluated by ultrasonography to assess prostatic conditions. Two different GnRH stimulation protocols were used, A = gonadorelin 50μg/dog SC (n = 15) and B = buserelin 0.12 μg/kg IV (n = 13). T and CPSE concentrations were measured before and 1 h after GnRH administration by a laser‐induced fluorescence analysis. Buserelin and gonadorelin were equally effective in causing a significant increase in serum T concentrations in the post GnRH sample. When considering the 28 dogs together, CPSE concentrations did not change following the stimulation test with either GnRH compound; however, in 4/28 cases, the post GnRH value was markedly increased to values compatible with a diagnosis of BPH. There was no difference in the action of buserelin or gonadorelin in causing an increase in serum T concentrations. CPSE secretion was increased in approximately 15% of dogs treated with either buserelin or gonadorelin. Therefore, whenever performing diagnostic testing in intact male dogs, CPSE should not be assayed on a post‐GnRH serum sample.
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