Urban morphology is a crucial contributor to urban heat island (UHI) effects. However, few studies have explored the complex effect of 2D/3D urban morphology on UHI from a multiscale perspective. In this study, We chose the central area of Jinan city, which was commonly known as the "furnace", as the case study area. novel 2D/3D urban morphology indexes-building coverage ratio (BCR)(for assessing the 2D building density), building volume density (BVD)( for assessing the 3D building density), and the frontal area index (FAI)(for assessing 3D ventilation conditions) were calculated and derived to investigated complexity of relationship between 2D/3D urban morphology and land surface temperature(LST) at different scales using the maximum information coefficient (MIC) and geographically weighted regression (GWR). The results indicated that (1) These newly 2D/3D urban morphology indexes as essential factors that are responsible for LST variation, BCR is the most important urban morphology index affecting the LST, followed by BVD and FAI. Importantly, the relationship between the BCR, BVD, and FAI and the LST was an inverse U-shaped curve. (2) The relationship between 2D/3D urban morphology and LST variation showed a significant scale effect. With increased grid size, the correlation between the BCR, BVD, and FAI and the LST strengthened, "inflection point" of inverse U-shaped curve was significantly declined, and their explanation rate to LST first increased and then decreased, with a maximum value at the 700-m scale. Additionally, the FAI exerted a stronger negative effect, while the BCR and BVD generally had stronger positive effects on LST as the grid size increasing. This study extends our scientific understanding of the complexity effect of urban morphology on LST and is of great practical significance for urban thermal environment regulation at multi-scale.