Uranium(VI) bio-coordination chemistry from biochemical, solution and protein structural data

Horn, J. David Van; Huang, Huan Chang

doi:10.1016/j.ccr.2005.09.010

Cited by 185 publications

(63 citation statements)

References 60 publications

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“…The uranium binding can produce conformational changes in proteins (Gray, 1994; Ghosh & Rosen, 2002;Yazzie et al, 2003). Uranium ions can generate ligands with functional groups of thiolates as well as carboxylate from acidic amino acids, such as aspartate or glutamate (Ghosh & Rosen, 2002;Van Horn & Huang, 2006;Zobel & Beer, 1961). Enzymes in G. sulfurreducens that assist in protein folding may help to avoid these potential deleterious effects.…”

Section: Protein and Dna Damagementioning

confidence: 99%

“…sulfurreducens is an aerotolerant anaerobe, with effective mechanisms for dealing with oxidative stress (Lin et al, 2004;Van Horn & Huang, 2006). Many proteins that are involved in the typical oxidative stress response in bacteria are also induced in response to other environmental stimuli, such as heat (Geslin et al, 2001;Privalle & Fridovich, 1987), high salt concentrations (Bernhardt et al, 1997) and heavy metals stress (Bernhardt et al, 1997;Chourey et al, 2006;Geslin et al, 2001;Roux & Covés, 2002).…”

Section: Proteins Involved In Oxidative Stressmentioning

confidence: 99%

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Proteome of Geobacter sulfurreducens in the presence of U(VI)

et al. 2014

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Geobacter species often play an important role in the in situ bioremediation of uranium-contaminated groundwater, but little is known about how these microbes avoid uranium toxicity. To evaluate this further, the proteome of Geobacter sulfurreducens exposed to 100 µM U(VI) acetate was compared to control cells not exposed to U(VI). Of the 1363 proteins detected from these cultures, 203 proteins had higher abundance during exposure to U(VI) compared with the control cells and 148 proteins had lower abundance. U(VI)-exposed cultures expressed lower levels of proteins involved in growth, protein and amino acid biosynthesis, as well as key central metabolism enzymes as a result of the deleterious effect of U(VI) on the growth of G. sulfurreducens. In contrast, proteins involved in detoxification, such as several efflux pumps belonging to the RND (resistance–nodulation–cell division) family, and membrane protection, and other proteins, such as chaperones and proteins involved in secretion systems, were found in higher abundance in cells exposed to U(VI). Exposing G. sulfurreducens to U(VI) resulted in a higher abundance of many proteins associated with the oxidative stress response, such as superoxide dismutase and superoxide reductase. A strain in which the gene for superoxide dismutase was deleted grew more slowly than the WT strain in the presence of U(VI), but not in its absence. The results suggested that there is no specific mechanism for uranium detoxification. Rather, multiple general stress responses are induced, which presumably enable Geobacter species to tolerate high uranium concentrations.

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Section: Protein and Dna Damagementioning

confidence: 99%

Section: Proteins Involved In Oxidative Stressmentioning

confidence: 99%

Proteome of Geobacter sulfurreducens in the presence of U(VI)

et al. 2014

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“…Recently, separation of uranium from diluted aqueous solution has been playing important role in separation sciences and has been engaging more attention than before (De 2000;Gupta and Singh 2003) with regard to environmental impact. Release of uranium-bearing aqueous solution from uranium material processing plant is found to be one of the major sources of environmental contaminants with the radioactive material, which is required to be separated to avoid a detrimental effect to aquatic life as well as human beings (Liao et al 2004;Van Horn and Huang 2006;Li et al 2010;Zhang et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Extraction of uranium from nuclear industrial effluent using polyacrylhydroxamic acid sorbent

Satpati

Pal

Goswami

et al. 2013

Int. J. Environ. Sci. Technol.

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Solid-phase extraction process using chelating sorbent is a novel technique due to its high separation efficiency and simplicity. A designed three-dimensional crosslinked hydrophilic chelating polymeric sorbent, polyacrylhydroxamic acid, was developed for enhanced uptake of uranium from waste solution (pH 6-9) by complexation between the sorbent's active functional group and uranium. The sorbent was synthesized by polymerization of acrylamide with cross-linking agent, followed by conversion and functionalization with hydroxylamine hydrochloride. In this paper, removal and recovery of uranium from effluent of uranium material processing plant in the presence of competitive ions such as sodium, calcium and magnesium were studied using the sorbent. Uranium uptake property of the developed sorbent was also investigated with respect to sorbent's physical characteristics such as bead size distribution and bead swelling, in batch experiments. Distribution coefficient of uranium in the sorbent was substantially high (1,250 mL of effluent/g of sorbent), and immobilization factor was 0.028. The results showed that more than 90 % recovery of uranium is viable from nuclear effluent without preconditioning. Breakthrough profiles of column operation were successfully described for effective removal of uranium in continuous mode. The novel sorbent has been used for polishing the nuclear wastewater and hence to mitigate the environmental issues.

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“…[3] Uranyl compounds are also neurotoxins. In mammals, uranyl ion (UO 2 2 + ) is mainly complexed by blood-transferring [4] and by low molecular weight complexing agents such as citrates, bicarbonates and phosphates.…”

mentioning

confidence: 99%

“…The hemoglobin (Hb) microspheres fabricated by the porous template covalent layer-by-layer (LbL) assembly were utilized as artificial oxygen carriers and blood substitutes. Magnetic nanoparticles of iron oxide (Fe 3 O 4 ) were loaded in porous CaCO 3 particles for magnetically assisted chemical separation (MACS). Through the adsorption spectrum of magnetic Hb microspheres after adsorbing UO 2 2 + , it was found that UO 2 2 + was highly loaded in the magnetic Hb microspheres, and it shows that the presence of UO 2 2 + in vivo destroys the structure and oxygen-transporting capability of Hb microspheres.…”

mentioning

confidence: 99%

High Impact of Uranyl Ions on Carrying–Releasing Oxygen Capability of Hemoglobin‐Based Blood Substitutes

Jia

et al. 2014

Chemistry A European J

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The effect of radioactive UO2 (2+) on the oxygen-transporting capability of hemoglobin-based oxygen carriers has been investigated in vitro. The hemoglobin (Hb) microspheres fabricated by the porous template covalent layer-by-layer (LbL) assembly were utilized as artificial oxygen carriers and blood substitutes. Magnetic nanoparticles of iron oxide (Fe3 O4 ) were loaded in porous CaCO3 particles for magnetically assisted chemical separation (MACS). Through the adsorption spectrum of magnetic Hb microspheres after adsorbing UO2 (2+) , it was found that UO2 (2+) was highly loaded in the magnetic Hb microspheres, and it shows that the presence of UO2 (2+) in vivo destroys the structure and oxygen-transporting capability of Hb microspheres. In view of the high adsorption capacity of UO2 (2+) , the as-assembled magnetic Hb microspheres can be considered as a novel, highly effective adsorbent for removing metal toxins from radiation-contaminated bodies, or from nuclear-power reactor effluent before discharge into the environment.

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Uranium(VI) bio-coordination chemistry from biochemical, solution and protein structural data

Cited by 185 publications

References 60 publications

Proteome of Geobacter sulfurreducens in the presence of U(VI)

Proteome of Geobacter sulfurreducens in the presence of U(VI)

Extraction of uranium from nuclear industrial effluent using polyacrylhydroxamic acid sorbent

High Impact of Uranyl Ions on Carrying–Releasing Oxygen Capability of Hemoglobin‐Based Blood Substitutes

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