Uptake, tissue distribution and toxicological effects of environmental microplastics in early juvenile fish Dicentrarchus labrax

Zitouni, Nesrine; Bousserrhıne, Noureddine; Missawi, Omayma; Boughattas, Iteb; Chèvre, Nathalie; Santos, Raphaël; Belbekhouche, Sabrina; Alphonse, Vanessa; Tisserand, Floriane; Balmassiere, Ludivine; Santos, Sofia Pereira Dos; Mokni, Moncef; Guerbej, Hamadi; Bannı, Mohamed

doi:10.1016/j.jhazmat.2020.124055

Cited by 104 publications

(38 citation statements)

References 80 publications

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“…6.16%, 1.53 and 0.46%, respectively) suggested that the smaller the particles, the greater the proportion of PS particles that enter the body. This indicates a ubiquitous size dependency in regard to PS bioavailability in organisms, as similar effects have also been reported in zebrafish [ 20 ], early juvenile fish [ 21 ], in vitro intestinal cell models [ 22 ], and rats [ 23 ]. However, conflicting findings have also been reported.…”

Section: Discussionsupporting

confidence: 66%

“…The color bars were used for grouping, one bar represented a dose of 250 mg/kg body weight, two bars with same color represented a dose of 500 mg/kg body weight, and two bars with different colors represented the mix exposure group with a dose of 250 mg/kg body weight of each particle. PC, pathological change; MU, mucin secretion; AP, apoptosis; TJ, tight junction; UC, unchanged; CR, compensatory response fish [21], in vitro intestinal cell models [22], and rats [23]. However, conflicting findings have also been reported.…”

Section: Discussionmentioning

confidence: 99%

“…However, particles > 1.5 μm rarely penetrate into the organs, and thus are unlikely to cause any organ damage [15]. The absorption of micro-and nanoplastics increases in organisms as particle size decreases [20][21][22][23]. Despite what is known, more research is needed on the adverse effects of microplastics and nanoplastics on human health.…”

Section: Introductionmentioning

confidence: 99%

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Underestimated health risks: polystyrene micro- and nanoplastics jointly induce intestinal barrier dysfunction by ROS-mediated epithelial cell apoptosis

et al. 2021

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Background Micro- and nanoplastic pollution has become a global environmental problem. Nanoplastics in the environment are still hard to detect because of analysis technology limitations. It is believed that when microplastics are found in the environment, more undetected nanoplastics are around. The current “microplastic exposure” is in fact the mixture of micro- and nanoplastic exposures. Therefore, the biological interaction between organisms among different sizes of micro- and nanoplastics should not be neglected. Results We measured the biodistribution of three polystyrene (PS) particles (50 nm PS, PS50; 500 nm PS, PS500; 5000 nm PS, PS5000) under single and co-exposure conditions in mice. We explored the underlying mechanisms by investigating the effects on three major components of the intestinal barrier (the mucus layer, tight junctions and the epithelial cells) in four intestine segments (duodenum, jejunum, ileum and colon) of mice. We found that the amounts of both PS500 and PS5000 increased when they were co-exposed with PS50 for 24 h in the mice. These increased amounts were due primarily to the increased permeability in the mouse intestines. We also confirmed there was a combined toxicity of PS50 and PS500 in the mouse intestines. This manifested as the mixture of PS50 and PS500 causing more severe dysfunction of the intestinal barrier than that caused by PS50 or PS500 alone. We found that the combined toxicity of PS micro- and nanoplastics on intestinal barrier dysfunction was caused primarily by reactive oxygen species (ROS)-mediated epithelial cell apoptosis in the mice. These findings were further confirmed by an oxidants or antioxidants pretreatment study. In addition, the combined toxicity of PS micro- and nanoplastics was also found in the mice after a 28-day repeated dose exposure. Conclusions There is a combined toxicity of PS50 and PS500 in the mouse intestines, which was caused primarily by ROS-mediated epithelial cell apoptosis in the mice. Considering that most recent studies on PS micro- and nanoplastics have been conducted using a single particle size, the health risks of exposure to PS micro- and nanoplastics on organisms may be underestimated.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Underestimated health risks: polystyrene micro- and nanoplastics jointly induce intestinal barrier dysfunction by ROS-mediated epithelial cell apoptosis

et al. 2021

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“…An oxidative stress status induced by microplastics was also detected on larvae gills after dietary exposures [140]. According to Zitouni et al [129], oxidative alterations were highly correlated with MPs size range. Moreover, the authors emphasized that the toxicity of smaller MPs was closely related to other different factors, including the target tissue, exposure duration, and MPs chemical properties.…”

Section: Occurrence Of Micro and Nano-plastics In Fish Gills And Consequent Effectsmentioning

confidence: 91%

“…Like fish collected in fish farm and mariculture areas, those exposed to MPs in laboratory conditions show a higher or, at most, a comparable accumulation of MPs in gills than in gut [127,128]. Therefore the MPs accumulation in gills could be influenced by microplastics shape [126], and the entity of accumulation in gills increases with increasing time of exposure [40] and is related to MPs size [129]. It is intriguing to notice that, once taken up, microplastics still exist in gill fishes after depuration for seven days [130].…”

Section: Occurrence Of Micro and Nano-plastics In Fish Gills And Consequent Effectsmentioning

confidence: 99%

Micro and Nano Plastics Distribution in Fish as Model Organisms: Histopathology, Blood Response and Bioaccumulation in Different Organs

et al. 2021

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Micro- and nano-plastic (MP/NP) pollution represents a threat not only to marine organisms and ecosystems, but also a danger for humans. The effects of these small particles resulting from the fragmentation of waste of various types have been well documented in mammals, although the consequences of acute and chronic exposure are not fully known yet. In this review, we summarize the recent results related to effects of MPs/NPs in different species of fish, both saltwater and freshwater, including zebrafish, used as model organisms for the evaluation of human health risk posed by MNPs. The expectation is that discoveries made in the model will provide insight regarding the risks of plastic particle toxicity to human health, with a focus on the effect of long-term exposure at different levels of biological complexity in various tissues and organs, including the brain. The current scientific evidence shows that plastic particle toxicity depends not only on factors such as particle size, concentration, exposure time, shape, and polymer type, but also on co-factors, which make the issue extremely complex. We describe and discuss the possible entry pathways of these particles into the fish body, as well as their uptake mechanisms and bioaccumulation in different organs and the role of blood response (hematochemical and hematological parameters) as biomarkers of micro- and nano-plastic water pollution.

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Ingestion of Microplastics by Marine Animals

Courtene‐Jones

Clark

Fischer

et al. 2022

Plastics and the Ocean

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Uptake, tissue distribution and toxicological effects of environmental microplastics in early juvenile fish Dicentrarchus labrax

Cited by 104 publications

References 80 publications

Underestimated health risks: polystyrene micro- and nanoplastics jointly induce intestinal barrier dysfunction by ROS-mediated epithelial cell apoptosis

Underestimated health risks: polystyrene micro- and nanoplastics jointly induce intestinal barrier dysfunction by ROS-mediated epithelial cell apoptosis

Micro and Nano Plastics Distribution in Fish as Model Organisms: Histopathology, Blood Response and Bioaccumulation in Different Organs

Ingestion of Microplastics by Marine Animals

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