Ingestion of Microplastics by Marine Animals

Courtene‐Jones, Winnie; Clark, Nathaniel J.; Fischer, Astrid; Smith, Natalie S.; Thompson, Richard C.

doi:10.1002/9781119768432.ch12

Cited by 16 publications

(6 citation statements)

References 140 publications

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“…Marine ecosystems, for instance, are known reservoirs of microplastics (Andrady, 2011;Cole et al, 2011;Ivar do Sul and Costa, 2014). This results in bioaccumulation within marine life, such as fish and oysters (Galloway et al, 2017;Sharma and Chatterjee, 2017;Bhuyan, 2022;Courtene-Jones et al, 2022). When such marine organisms serve as the source animals for cell extraction, undetected microplastics could be inadvertently introduced into the cultivation process.…”

Section: Introductionmentioning

confidence: 99%

Enhancing food safety and cultivated meat production: exploring the impact of microplastics on fish muscle cell proliferation and differentiation

Sun,

Timoneda,

Banavar

et al. 2024

Front. Food. Sci. Technol.

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Cultivated meat, a sustainable alternative to traditional livestock farming, has gained attention for its potential environmental and health benefits. However, concerns about microplastic contamination pose challenges, especially when sourcing cells from marine organisms prone to microplastic bioaccumulation. Additionally, the pervasive presence of microplastics in laboratory settings, ingredients, and during the production, increases the risk of unintentional contamination. This study focused on Atlantic mackerel (Scomber scombrus) skeletal muscle cell lines to examine the effects of microplastic exposure, represented by fluorescent polyethylene microspheres (10–45 µm) on cell performance including cell proliferation, cell viability, gene expression, and differentiation processes critical for cultivated meat production. The results revealed significant impacts on cell attachment and proliferation at microplastic concentrations of 1 μg/mL, 10 μg/mL, and 50 μg/mL. Notably, the 10 μg/mL concentration exerted the most pronounced effects on cell viability during both attachment and proliferation phases. While the results indicated that both microplastic concentration and size influence cell viability, cell differentiation remained unaffected, and additional contributing factors require further investigation. These findings underscore the necessity of thoroughly exploring microplastic-cell interactions to ensure food safety and safeguard health within the burgeoning cultivated meat industry.

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Section: Introductionmentioning

confidence: 99%

Enhancing food safety and cultivated meat production: exploring the impact of microplastics on fish muscle cell proliferation and differentiation

Sun,

Timoneda,

Banavar

et al. 2024

Front. Food. Sci. Technol.

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“…Studying MPs in gastropods can indicate MP contamination in freshwater systems. Additionally, it serves as a bioindicator of environmental pollutants because humans are the primary consumers in the food chain, raising awareness of microplastic pollution and food safety ( Azhari et al, 2022 ; Multisanti et al, 2022 ; Cera et al, 2022 ; Hu, Shen & Tang, 2022 ; Courtene-Jones et al, 2022 ). To date, there have been limited studies on MP contamination in freshwater gastropods living in rivers in tropical areas.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of microplastics in freshwater gastropods from a tropical river U-Taphao, southern Thailand

Jitkaew

Pradit

Noppradit

et al. 2023

PeerJ

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Background Microplastics (MPs) are pollutants in rivers and marine environments. Rivers can be sources and sinks of MPs that enter the biota. Previous studies focusing on freshwater species are quite limited, especially for gastropods. Freshwater gastropods are essential to aquatic ecosystems because they are food to other aquatic animals, such as fish, shrimp, and crabs. They are a crucial link in the food chain between water resources and human food. Therefore, this study aimed to investigate MP accumulation in freshwater gastropods, commonly known as snails (Filopaludina sumatrensis speciosa and Pomacea canaliculata), in a river flowing into a shallow coastal lagoon. Method In this study, snail tissue samples were digested with 30% hydrogen peroxide. The mixture was heated at 60 °C for 24 h. MP particles were identified, counted, and characterized (shape, size, and color) by visual identification under a stereomicroscope. Furthermore, polymer-type identification was performed using Fourier transform infrared spectroscopy (FTIR). Analysis of variance (ANOVA) was applied for the statistical analysis. Results The MPs found were as follows: 4.76 particles/individual were found in F. sumatrensis speciosa upstream, 5.20 particles/individual were found in F. sumatrensis speciosa downstream, 7.28 particles/individual were found in P. canaliculata upstream, and 4.00 particles/individual were found in P. canaliculata downstream. It was found in the two-way ANOVA that the accumulation of MPs in gastropods was affected by species and study sites (upstream and downstream). There was a significant difference in the amount of MPs in P. canaliculata between upstream and downstream sites (p = 0.003). Fibers were the most common MPs in both species. Moreover, P. canaliculata upstream had the most significant amount of MPs. The smallest amount of MPs was recorded for P. canaliculata downstream, but there was great diversity in shape, size, and polymer type. MPs sized 500 μm–1 mm were the most common in both species. Fourier transform infrared spectroscopy revealed six polymers: poly (ethylene terephthalate), polypropylene, rayon, polyethyleneimine, polyamine, and poly (propylene: ethylene). The occurrence of MPs in gastropods is alarming for food security in Thailand. The results of this study can be used to support baseline data on MP accumulation among freshwater gastropods.

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“…Marine ecosystems, for instance, are known reservoirs of microplastics (Andrady, 2011; Cole et al, 2011; Ivar do Sul & Costa, 2014). This results in bioaccumulation within marine life, such as fish and oysters (Bhuyan, 2022; Courtene-Jones et al, 2022; Galloway et al, 2017; Sharma & Chatterjee, 2017). When such marine organisms serve as the source animals for cell extraction, undetected microplastics could be inadvertently introduced into the cultivation process.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigating the Impact of Microplastics on Fish Muscle Cell Proliferation and Differentiation: Enhancing Food Safety in Cultivated Meat Production

Sun,

Timoneda,

Banavar

et al. 2023

Preprint

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Cultivated meat, a sustainable alternative to traditional livestock farming, has gained attention for its potential environmental and health benefits. However, concerns about microplastic contamination pose challenges, especially when sourcing cells from marine organisms prone to microplastic bioaccumulation. Additionally, the pervasive presence of microplastics in laboratory settings, ingredients, and during the production, increases the risk of unintentional contamination. This study focused on Atlantic mackerel (Scomber scombrus) skeletal muscle cell lines to examine the effects of microplastic exposure, represented by fluorescent polyethylene microspheres (10-45 um) on cell performance including cell proliferation, cell viability, gene expression, and differentiation processes critical for cultivated meat production. The results revealed significant impacts on cell attachment and proliferation at microplastic concentrations of 1 ug/mL, 10 ug/mL, and 50 ug/mL. Notably, the 10 ug/mL concentration exerted the most pronounced effects on cell viability during both attachment and proliferation phases. While the results indicated that both microplastic concentration and size influence cell viability, cell differentiation remained unaffected, and additional contributing factors require further investigation. These findings underscore the necessity of thoroughly exploring microplastic-cell interactions to ensure food safety and safeguard health within the burgeoning cultivated meat industry.

show abstract

Ingestion of Microplastics by Marine Animals

Cited by 16 publications

References 140 publications

Enhancing food safety and cultivated meat production: exploring the impact of microplastics on fish muscle cell proliferation and differentiation

Enhancing food safety and cultivated meat production: exploring the impact of microplastics on fish muscle cell proliferation and differentiation

Occurrence of microplastics in freshwater gastropods from a tropical river U-Taphao, southern Thailand

Investigating the Impact of Microplastics on Fish Muscle Cell Proliferation and Differentiation: Enhancing Food Safety in Cultivated Meat Production

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