Uptake route and resulting toxicity of silver nanoparticles in Eisenia fetida earthworm exposed through Standard OECD Tests

García-Velasco, Nerea; Gandariasbeitia, Maite; Irizar, Amaia; Soto, Manu

doi:10.1007/s10646-016-1710-2

Cited by 53 publications

(23 citation statements)

References 42 publications

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“…Antisari et al [ 223 ] reported for Co NP a reduction in soil microbial biomass and changes in its community composition, which may partly explain alterations in earthworms’ fatty acid composition. The uptake of TiO 2 NP via contaminated food seems to be of high relevance for terrestrial [ 224 , 225 ] and aquatic invertebrates [ 226 ]. This direct uptake pathway increases NP exposure and might influence the food quality through impacts on food-associated microorganisms.…”

Section: Np Effects In Communities and Consequences For Trophic Intermentioning

confidence: 99%

Nanoparticles in the environment: where do we come from, where do we go to?

et al. 2018

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Nanoparticles serve various industrial and domestic purposes which is reflected in their steadily increasing production volume. This economic success comes along with their presence in the environment and the risk of potentially adverse effects in natural systems. Over the last decade, substantial progress regarding the understanding of sources, fate, and effects of nanoparticles has been made. Predictions of environmental concentrations based on modelling approaches could recently be confirmed by measured concentrations in the field. Nonetheless, analytical techniques are, as covered elsewhere, still under development to more efficiently and reliably characterize and quantify nanoparticles, as well as to detect them in complex environmental matrixes. Simultaneously, the effects of nanoparticles on aquatic and terrestrial systems have received increasing attention. While the debate on the relevance of nanoparticle-released metal ions for their toxicity is still ongoing, it is a re-occurring phenomenon that inert nanoparticles are able to interact with biota through physical pathways such as biological surface coating. This among others interferes with the growth and behaviour of exposed organisms. Moreover, co-occurring contaminants interact with nanoparticles. There is multiple evidence suggesting nanoparticles as a sink for organic and inorganic co-contaminants. On the other hand, in the presence of nanoparticles, repeatedly an elevated effect on the test species induced by the co-contaminants has been reported. In this paper, we highlight recent achievements in the field of nano-ecotoxicology in both aquatic and terrestrial systems but also refer to substantial gaps that require further attention in the future.

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Section: Np Effects In Communities and Consequences For Trophic Intermentioning

confidence: 99%

Nanoparticles in the environment: where do we come from, where do we go to?

et al. 2018

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“…When combined with the dermal absorption of Ag + ions, this increased metabolism could explain the higher toxicity of AgNO 3 . However, (Diez-Ortiz et al, 2015b;Garcia-Velasco et al, 2016) showed that Ag is mainly internalized by soil ingestion. Our bioaccumulation results show that Ag can enter the earthworm body.…”

Section: Discussionmentioning

confidence: 99%

“…Inf 1). Four different Ag concentrations, C1, C2, C3 and C4, were used for the AgNP and AgNO 3 microcosms: 30 (± 20), 70 (± 10), 120 (± 15) and 280 (± 40) mg kg -1 (dry matter), respectively (these concentrations were chosen based on mortality rates reported in Garcia-Velasco et al (2016) and Gomes et al (2015)). Four different volumes of NM300K dispersant were used in the dispersant microcosms, which served as controls.…”

Section: Experimental Scheme (Earthworm Exposure)mentioning

confidence: 99%

Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida

Courtois¹,

Rorat²,

Lemière³

et al. 2020

Environ Sci Pollut Res

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The use of silver nanoparticles (AgNPs) in agriculture and many consumer products has led to significant release of Ag in the environment. Although Ag toxicity in terrestrial organisms has been studied extensively, very little is known about the accumulation capacity and coping mechanisms of organisms in Agcontaminated soil. In this context, we exposed Eisenia fetida earthworms to artificial OECD soil spiked with a range of concentrations of Ag (AgNPs or AgNO 3 ). The main aims were to (1) identify the location and form of accumulation of Ag in the exposed earthworms and (2) better understand the physiological mechanisms involved in Ag detoxification. The results showed that similar doses of AgNPs or AgNO 3 did not have the same effect on E. fetida survival. The two forms of Ag added to soil exhibited substantial differences in speciation at the end of exposure, but the Ag speciation and content of Ag in earthworms were similar, suggesting that biotransformation of Ag occurred. Finally, 3D images of intact earthworms obtained by X-ray micro-computed tomography revealed that Ag accumulated preferentially in the chloragogen tissue, coelomocytes and nephridial epithelium.Thus, E. fetida bioaccumulates Ag, but a regulation mechanism limit its impact in a very efficient manner. The location of Ag in the organism, the competition between Ag and Cu, and the speciation of internal Ag suggest a link between Ag and the thiol-rich proteins that are widely present in these tissues, most probably metallothioneins, which are key proteins in the sequestration and detoxification of metals.

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“…Cells exposed to other nanoparticles, such as Ag NPs, nZVI NPs or ZnO NPs release significantly greater ROS amounts. Contrary to TiO 2 NPs, ROS production could be elicited by the metal ions released from these nanoparticles [11,29,30].…”

Section: Mrna Levels Of Detoxification Immune Antioxidant and Signal Transduction Moleculesmentioning

confidence: 99%

In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment

et al. 2021

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Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cells exposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 µg/mL) for 2, 6, and 24 h. TiO2 NPs at all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactive oxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity. Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPs at the cellular level. At the gene expression level, slight changes were detected. Metallothionein, fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to 10 µg/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells. TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strong effects of these nanoparticles on coelomocytes at both the cellular and molecular levels.

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Uptake route and resulting toxicity of silver nanoparticles in Eisenia fetida earthworm exposed through Standard OECD Tests

Cited by 53 publications

References 42 publications

Nanoparticles in the environment: where do we come from, where do we go to?

Nanoparticles in the environment: where do we come from, where do we go to?

Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida

In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment

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