Uptake of silica particulate drug carriers in an intestine-on-a-chip: towards a better <i>in vitro</i> model of nanoparticulate carrier and mucus interactions

Pocock, Kyall; Delon, Ludivine; Khatri, Aparajita; Prestidge, Clive A.; Gibson, Rachel J.; Barbe, Chris; Thierry, Benjamin

doi:10.1039/c9bm00058e

Cited by 28 publications

(20 citation statements)

References 60 publications

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“…According to previous reports, the mucus layer of organs often allows smaller particles to enter and block larger ones. 33 Therefore, the nanoparticles prepared by microfluidics may have better absorption characteristics. In addition, as illustrated in Figure 2c, the PDI of the nanoparticles prepared by microfluidics was less than 0.15 and the yield of FX/SH NPs was about 1.4 mg/ min, which was much smaller than that of the nanoparticles prepared by bulk mixing.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Microfluidic Fabrication of pH-Responsive Nanoparticles for Encapsulation and Colon-Target Release of Fucoxanthin

Liang

Zhao

et al. 2021

J. Agric. Food Chem.

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Improving the stability of fucoxanthin in the gastrointestinal tract is an important approach to enhance its oral bioavailability. The study proposed a new microfluidic device allowing for the synthesis of a structurally well-defined nanoscale delivery system with a uniform size for encapsulation and colon-target release of fucoxanthin. The rapid mixing in the microfluidic channel ensured that the mixing time was shorter than the aggregation time, thus realizing the controllable control of the coprecipitation of fucoxanthin and shellac polymer. In vitro digestion tests showed that a pH stimulus-responsive release of fucoxanthin from FX/SH NPs was observed under alkaline pH conditions. The fluorescence colocalization imaging indicated that FX/SH NPs did not affect the intestine function and had a protective effect on Caco-2 cells damaged by H2O2 by enhancing their antioxidant capacity. Overall, this work illustrated the promise of using a microfluidic approach to fabricate the biomimetic nanodelivery system for better biocompatibility and targeting efficacy.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Microfluidic Fabrication of pH-Responsive Nanoparticles for Encapsulation and Colon-Target Release of Fucoxanthin

Liang

Zhao

et al. 2021

J. Agric. Food Chem.

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“…Caco‐2 cells monolayers displayed a dense F‐actin network ( Figure A), high level of tight junctions (ZO‐1 protein expression shown in Figure 5C), and high numbers of active mitochondria (Figure 5D). These observations are in excellent agreement with Caco‐2 monolayers cultured under an identical FSS applied using an external pump [ 4,20,23 ] (Figure 5) and demonstrates that efficient differentiation occurs within 5 days of culture in the pumpless device.…”

Section: Resultsmentioning

confidence: 99%

Unlocking the Potential of Organ‐on‐Chip Models through Pumpless and Tubeless Microfluidics

Delon

Nilghaz

Cheah

et al. 2020

Adv Healthcare Materials

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Microfluidic organs‐on‐chips are rapidly being developed toward eliminating the shortcomings of static in vitro models and better addressing basic and translational research questions. A critical aspect is the dynamic culture environment they provide. However, the associated inherent requirement for controlled fluid shear stress (FSS) and therefore the need for precise pumps limits their implementation. To address this issue, here a novel approach to manufacture pumpless and tubeless organs‐on‐chips is reported. It relies on the use of a hydrophilic thread to provide a driving force for the perfusion of the cell culture medium through constant evaporation in the controlled conditions of a cell incubator. Well‐defined and tuneable flow rates can be applied by adjusting the length and/or diameter of the thread. This approach for the preparation of an intestine‐on‐chip model based on the Caco‐2 cell line is validated. Five days culture under 0.02 dyn·cm−2 shear conditions yield monolayers similar to those prepared using a high‐precision peristaltic pump. A pumpless device can also be used to delineate the effect of FSS on the phenotype of adenocarcinomic human alveolar basal epithelial A549 cells. It is anticipated that the pumpless approach will facilitate and herefore increase the use of organs‐on‐chips models in the future.

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“…Key breakthroughs and developments in intestinal epithelium culture in on-chip devices include the in vitro recapitulation of crypt–villus architecture, − barrier function, ,, and mucus layer formation. , To form the crypt–villus architecture in microfluidics, for example, the Ingber group developed a device featuring two parallel hollow chambers separated by a porous extracellular matrix (ECM) coated membrane (Figure A) . Using this multichamber setup, they grew biopsy-derived human intestinal epithelial cells to confluence on one side while applying fluid flow on the apical surface of cells and cyclic stresses to induce the spontaneous formation of folded structures similar to the crypt–villus shape. − Subsequent research indicates a direct role for shear stress in inducing further differentiation of intestinal epithelial cells, which highlights the unique capacity for the multichamber microfluidic design for achieving robust expansion and differentiation of intestinal epithelium in vitro …”

Section: Construction Of Brain-on-a-chip and Gut–brain Axis (Gba) Modelsmentioning

confidence: 99%

Emerging Roles of Microfluidics in Brain Research: From Cerebral Fluids Manipulation to Brain-on-a-Chip and Neuroelectronic Devices Engineering

et al. 2022

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Remarkable progress made in the past few decades in brain research enables the manipulation of neuronal activity in single neurons and neural circuits and thus allows the decipherment of relations between nervous systems and behavior. The discovery of glymphatic and lymphatic systems in the brain and the recently unveiled tight relations between the gastrointestinal (GI) tract and the central nervous system (CNS) further revolutionize our understanding of brain structures and functions. Fundamental questions about how neurons conduct two-way communications with the gut to establish the gut–brain axis (GBA) and interact with essential brain components such as glial cells and blood vessels to regulate cerebral blood flow (CBF) and cerebrospinal fluid (CSF) in health and disease, however, remain. Microfluidics with unparalleled advantages in the control of fluids at microscale has emerged recently as an effective approach to address these critical questions in brain research. The dynamics of cerebral fluids (i.e., blood and CSF) and novel in vitro brain-on-a-chip models and microfluidic-integrated multifunctional neuroelectronic devices, for example, have been investigated. This review starts with a critical discussion of the current understanding of several key topics in brain research such as neurovascular coupling (NVC), glymphatic pathway, and GBA and then interrogates a wide range of microfluidic-based approaches that have been developed or can be improved to advance our fundamental understanding of brain functions. Last, emerging technologies for structuring microfluidic devices and their implications and future directions in brain research are discussed.

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Uptake of silica particulate drug carriers in an intestine-on-a-chip: towards a better in vitro model of nanoparticulate carrier and mucus interactions

Abstract: An intestine-on-a-chip model was used for the first time to study the intestinal uptake of nanoparticulate oral drug carriers and their ability to overcome the mucus barrier.

Cited by 28 publications

References 60 publications

Microfluidic Fabrication of pH-Responsive Nanoparticles for Encapsulation and Colon-Target Release of Fucoxanthin

Microfluidic Fabrication of pH-Responsive Nanoparticles for Encapsulation and Colon-Target Release of Fucoxanthin

Unlocking the Potential of Organ‐on‐Chip Models through Pumpless and Tubeless Microfluidics

Emerging Roles of Microfluidics in Brain Research: From Cerebral Fluids Manipulation to Brain-on-a-Chip and Neuroelectronic Devices Engineering

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