Uptake of metabolizable sugars bySaccharomyces cerevisiae

Burger, M.; Hejmová, Libuše

doi:10.1007/bf02868229

Cited by 20 publications

(9 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on these observations, it is concluded that the uptake kinetic properties are pH dependent. This has been shown for sugar uptake in S. cerevisiae, Rhodotorula gracilis, and soybean, whereby the affinity coefficient (K m ) was found to increase at decreasing pH levels, while the maximum uptake rate decreased (7,25,31). Cassio and Leao (10) also reported that in C. utilis for succinic acid uptake.…”

Section: Resultsmentioning

confidence: 68%

pH-Dependent Uptake of Fumaric Acid in Saccharomyces cerevisiae under Anaerobic Conditions

Jamalzadeh

Verheijen

Heijnen

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

Microbial production of C 4 dicarboxylic acids from renewable resources has gained renewed interest. The yeast Saccharomyces cerevisiae is known as a robust microorganism and is able to grow at low pH, which makes it a suitable candidate for biological production of organic acids. However, a successful metabolic engineering approach for overproduction of organic acids requires an incorporation of a proper exporter to increase the productivity. Moreover, low-pH fermentations, which are desirable for facilitating the downstream processing, may cause back diffusion of the undissociated acid into the cells with simultaneous active export, thereby creating an ATP-dissipating futile cycle. In this work, we have studied the uptake of fumaric acid in S. cerevisiae in carbon-limited chemostat cultures under anaerobic conditions. The effect of the presence of fumaric acid at different pH values (3 to 5) has been investigated in order to obtain more knowledge about possible uptake mechanisms. The experimental results showed that at a cultivation pH of 5.0 and an external fumaric acid concentration of approximately 0.8 mmol · liter ؊1 , the fumaric acid uptake rate was unexpectedly high and could not be explained by diffusion of the undissociated form across the plasma membrane alone. This could indicate the presence of protein-mediated import. At decreasing pH levels, the fumaric acid uptake rate was found to increase asymptotically to a maximum level. Although this observation is in accordance with proteinmediated import, the presence of a metabolic bottleneck for fumaric acid conversion under anaerobic conditions could not be excluded.

show abstract

Section: Resultsmentioning

confidence: 68%

pH-Dependent Uptake of Fumaric Acid in Saccharomyces cerevisiae under Anaerobic Conditions

Jamalzadeh

Verheijen

Heijnen

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…4, for example), it seemed worthwhile t o investigate the permeability of the two yeasts to sugars a t various temperatures. Glucosamine was chosen as the substrate for the permeating mechanism because it accumulates in the cell and is easily determined, and its uptake has been fairly thoroughly studied (3).…”

Section: Injeuence Of Temperatz~re On Permeability To Glucosaminementioning

confidence: 99%

Observations on the Physiology of Psychrophilism in a Yeast

Baxter¹,

Gibbons²

1962

Can. J. Microbiol.

View full text Add to dashboard Cite

I n a psychrophilic Candida species, survival, respiration of intact cells, activity of alcohol dehydrogenase, and entry of glucosamine into the cell were more sensitive t o temperatures above 10" C than in a mesophilic species, Candida lipolylica. However, growth was more sensitive t o temperature than any of these other properties. Respiration of the psychrophile was more rapid than that of the mesophile a t all temperatures up to 30°, the difference being most striking a t Oolo0 C. I t is suggested that the upper temperature limit for growth of the psychrophile is set by the reversible inactivation of some vital reaction not yet identified.Uptalce of glucosa~nine in the psychrophile was rapid and the rate only slightly influenced by temperature between 0" and 30". I n contrast there was scarcely any uptake by the mesophile a t O0 and lo0, and the rate a t 20° was much lower than that a t 30". I t is suggested that the lower temperature limit of growth for the mesophile is set by the temperature dependence of the mechanisms for transporting substrates into the cell, and that a n increased permeability a t low temperatures may be an important factor in permitting some organisms to thrive in the cold.'Manuscript

show abstract

“…In S. cerevisiae, GlcN was reported to produce a repression similar to that exerted by glucose itself, probably as a glucose analogue. 41 ) Alternatively, accumulation of certain sugar phosphate in the mycelium, which inhibits macromolecular synthesis such as that of RNA, might occur. Maitra 42 ) reported that in a mutant of S. cerevisiae deficient in phosphoglucoisomerase, glucose caused accumulation of glucose-6-phosphate and suppressed synthesis of RNA.…”

Section: Effect Of Glen On Hyphal Morphologymentioning

confidence: 99%