Uptake of maternal immunoglobulins in the enterocytes of suckling piglets: improved detection with a streptavidin-biotin bridge gold technique.

Kömüves, L.G.; Heath, Julian P.

doi:10.1177/40.11.1431052

Cited by 23 publications

(14 citation statements)

References 14 publications

(18 reference statements)

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“…166 The first 24 hours after birth of the newborn pigs is exceptionally crucial for the transfer and intestinal absorption of maternal IgG from colostrum. [166][167][168] Although maternal antibodies through suckling can protect newborn piglets to some extent, the piglets are still highly susceptible to new infections. Highdensity commercial farming practices, where piglets are typically sent to market at 6 months of age, can therefore have profound effects on the transmission and diversity of IAVs in various swine populations.…”

Section: Pigs: the Mixing Vessel?mentioning

confidence: 99%

The ecology and adaptive evolution of influenza A interspecies transmission

Joseph

Vijaykrishna

et al. 2016

Influenza Resp Viruses

100

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Since 2013, there have been several alarming influenza‐related events; the spread of highly pathogenic avian influenza H5 viruses into North America, the detection of H10N8 and H5N6 zoonotic infections, the ongoing H7N9 infections in China and the continued zoonosis of H5N1 viruses in parts of Asia and the Middle East. The risk of a new influenza pandemic increases with the repeated interspecies transmission events that facilitate reassortment between animal influenza strains; thus, it is of utmost importance to understand the factors involved that promote or become a barrier to cross‐species transmission of Influenza A viruses (IAVs). Here, we provide an overview of the ecology and evolutionary adaptations of IAVs, with a focus on a review of the molecular factors that enable interspecies transmission of the various virus gene segments.

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Section: Pigs: the Mixing Vessel?mentioning

confidence: 99%

The ecology and adaptive evolution of influenza A interspecies transmission

Joseph

Vijaykrishna

et al. 2016

Influenza Resp Viruses

100

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“…Directly after birth the piglet takes up macromolecules from the intestinal lumen in a non-selective way. IgG from the sow's colostrum is absorbed by the newborn via enterocytes [22,23]. A rapid "closure" of the gut for the macromolecular uptake occurs within 24 and 48 h after birth [25].…”

Section: Gut Function and Immunitymentioning

confidence: 99%

Postnatal development of intestinal immune system in piglets: implications for the process of weaning

Stokes¹,

Bailey²,

Haverson³

et al. 2004

Anim. Res.

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-European-wide directives are in place to establish a sustainable production of pigs without using production enhancers and chemotherapeutics. Thus, an economically-viable pig production is now only possible when the physiological mechanisms of defense against pathogens and tolerance against nutrients and commensal bacteria in the intestinal immune system are taken into account. During the postnatal period the piglet is facing first the time large amounts of new antigens and at weaning a second wave of nutritional antigens is entering the intestinal tract. The appropriate development of humoral and cellular functions of the intestinal immune system is essential for optimum growth and performance of the piglets. The integrity of the intestinal surfaces is a prerequisite of intestinal immunity and tolerance. Secretory IgA serves to exclude harmful antigens from uptake. The induction of intestinal immune reactions starts with antigen presentation by professional antigen presenting cells of Peyer's patches and mesenteric lymph nodes. In addition, the intestinal lamina propria serves as a mucosal compartment for regulation of immune responses. Here especially T regulatory cells (CD4 + CD25 + ) have their function for maintaining intestinal homeostasis. The network of mucosal T and B cells develops after birth in a programmed sequence; it is almost completed at week 7 after birth. Weaning is associated with changes in the regulation of the lymphoid cells in the mucosa. In small and large intestine increases in pro-and anti-inflammatory cytokines were observed after weaning in lymphocytes. Epithelial cells were studied both in intestinal samples and in vitro. Here the cytokine patterns provide evidence that weaning is inducing a transient inflammation of the mucosa. Piglets weaned under conventional conditions have a thicker mucosa than pigs weaned from isolators. Cells of isolator-reared pigs show slightly higher levels of activation markers -probably * Corresponding author: hermann-josef.rothkoetter@medizin.uni-magdeburg.de 326 C.R. Stokes et al.reflecting the interaction of the foreign protein derived from bovine milk. The results presented in this overview demonstrate that further effort is necessary to elucidate the function of the porcine intestinal immune system in the postnatal period and at the time of weaning to provide criteria for porcine intestinal health.inflammation / intestinal immunity / mucosa / nutrition / pig / postnatal / weaning Résumé -Développement postnatal de l'immunité intestinale chez le porcelet : implications pour le processus de sevrage. Des directives européennes ont été adoptées, en vue de mettre en place une production durable de porcs sans facteurs de croissance ni antibiotiques. Dans ce contexte, un élevage porcin ne sera économiquement viable que si sont pris en compte les mécanismes physiologiques de défense contre les agents pathogènes, et de tolérance vis-à-vis des aliments et des bactéries commensales, mécanismes engageant le système immunitaire intestinal des animaux. ...

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“…We have shown in an earlier study, using immunocytochemical detection methods at both the light and EM levels (Komuves and Heath, 1992), that the endocytotic granules are enriched in maternal IgG. When, in a search for lenten cells, we re-examined the sections from that study, we noted that immunoglobulins were never seen in the cytoplasm of these cells.…”

Section: Absorptive Characteristics Of Lenten Cellsmentioning

confidence: 81%

Lenten cell: Ultrastructure, absorptive properties, and enzyme expression of a novel type of cell in the newborn and suckling pig intestinal epithelium

1996

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Background The small intestinal epithelium is made up of columnar absorptive enterocytes and a smaller number of specialized non‐absorptive cells, including goblet cells, enteroendocrine cells, M cells, cup cells, and tuft cells. During a study on milk protein absorption in newborn pigs, we identified an enterocyte that showed no uptake of milk proteins and that could be found only in the jejunum and ileum of pigs during the first 2 weeks of life. We call this previously undescribed enterocyte the lenten cell. Methods We used light microscopy and transmission electron microscopy in conjunction with immunolabelling and cytochemical techniques to determine the occurrence, ultrastructure, absorptive properties, and brush border hydrolase expression of lenten cells. Results Lenten cells constituted ∼1–2% of the villous epithelium. They were seen in newborn and suckling pigs 1–9 days of age, but were not found in weaned pigs. Morphologically, lenten cells were spindle‐ or wineglass‐shaped, with a ventrally sited nucleus and an electron‐dense cytoplasm with numerous cytokeratin filaments. Lenten cells had a normal brush border with microvilli that were slightly thicker than those of absorptive enterocytes, but they did not express the brush border hydrolases lactase, aminopeptidase N, and alkaline phosphatase. Lenten cells did not endocytose milk proteins or horseradish peroxidase, but contained some endocytic or secretory vacuoles and a few dense granules. Conclusions No role for lenten cells has been identified in this study, but presence of these cells during the neonatal period, when growth and differentiation of the gastrointestinal tract is at a peak, clearly suggests that lenten cells may play a role in this process. © 1996 Wiley‐Liss, Inc.

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Uptake of maternal immunoglobulins in the enterocytes of suckling piglets: improved detection with a streptavidin-biotin bridge gold technique.

Cited by 23 publications

References 14 publications

The ecology and adaptive evolution of influenza A interspecies transmission

The ecology and adaptive evolution of influenza A interspecies transmission

Postnatal development of intestinal immune system in piglets: implications for the process of weaning

Lenten cell: Ultrastructure, absorptive properties, and enzyme expression of a novel type of cell in the newborn and suckling pig intestinal epithelium

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