Uptake of HgCl2and MeHgCl in an Insect Cell Line (Aedes albopictusC6/36)

Braeckman, Bart P.; Cornelis, Rita; Rzeznik, Urszula; Raes, Hilda

doi:10.1006/enrs.1998.3841

Cited by 17 publications

(14 citation statements)

References 29 publications

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“…This agrees with a study by Lawson & Mason (1998) showing that inorganic mercury is readily retained in the pecto-cellulosic cell wall of unicellular algae, while the organic form appears quickly taken up. Braeckman et al (1998) demonstrated a similar distribution pattern of both compounds in insect cell cultures. Moreover, using autoradiography to reveal bioaccumulated Hg in the beetle, Lindqvist et al (1995) found that methylmercury is evenly distributed in most body tissues, whereas inorganic mercury stays confined to the interface organs.…”

Section: Discussionmentioning

confidence: 57%

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

Laporte¹,

Truchot

Mesmer-Dudons³

et al. 2002

Mar. Ecol. Prog. Ser.

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In order to better understand the role of the gills in the accumulation of contaminants, branchial uptake and bioaccumulation of inorganic (HgCl 2 ) and monomethylmercury (CH 3 HgCl) were quantified in the shore crab Carcinus maenas, using living animals and an in vitro perfused gill preparation exposed to 50 µg l -1 of either chemical forms in the external medium. In addition, localization of accumulated mercury was studied using the histochemical autometallographic technique by both light and electron microscopy. Gill tissue strongly accumulated either inorganic or methylated mercury at similar levels in vitro and in vivo. For both chemical forms of the metal, only about 1% of the total mercury input was recovered in the effluent fluid from in vitro perfused gills and could thus be considered available to distribute inside the animal via the circulatory system. Inorganic Hg was histochemically found to be accumulated at 2 markedly different locations in the gills: at the cuticular surface in direct contact with the contaminated medium and at high local levels in the central vacuole of gill nephrocytes. Although also present at these 2 locations, methylmercury was distributed more diffusely and more evenly in all cells. These results suggest that the high affinity of gill tissue for both forms of mercury may confer on this organ the role of an external barrier strongly limiting the invasion of the metal toward other compartments of the body.

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Section: Discussionmentioning

confidence: 57%

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

Laporte¹,

Truchot

Mesmer-Dudons³

et al. 2002

Mar. Ecol. Prog. Ser.

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“…The uptake of Cd was a very rapid process; the time necessary to reach half maximum uptake (T 0.5 ) was 37.5 min, compared to 130 min for HgCl 2 , a related heavy metal dichloride (Braeckman et al, 1998). The rapidity of the Cd accumulation process at the cellular level varies considerably among literature data.…”

Section: Discussionmentioning

confidence: 97%

“…As we found earlier (Braeckman et al, 1998) that viability decrease does not necessarily parallel metal accumulation, we also studied the temperature effect on Cd accumulation. In the viability experiments, Cd toxicity was proved to be temperature sensitive.…”

Section: Temperature Effect On CD Toxicity and Accumulationmentioning

confidence: 99%

Cadmium Uptake and Defense Mechanism in Insect Cells

Braeckman

Smagghe

Brutsaert

et al. 1999

Environmental Research

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“…Mercury competes for common transport and cellular sites in the body (Gochfeld, 1997); may inhibit enzyme systems (Bidstrup, 1964;Passino & Cotant, 1979); and shows a strong affinity for phosphates, cysteinyl and histidyl side chains of proteins, purines, and porphyrins (Corbett, 1974). Moreover, in some cell systems, the main cellular target for HgCl 2 is the plasma membrane, whereas methyl Hg more readily crosses this barrier and reacts with intracellular targets (Braeckman et al, 1998).…”

Section: Hg Metabolism Excretion and Mechanisms Of Actionmentioning

confidence: 99%

“…These include duration of exposure, time after cessation of exposure, intracellul ar compartmentation, cell type, and species (Fowler, 1987). Further work has emphasized that cellular uptake of Hg is inversely related to cell density; increases at higher exposure temperatures; and is influenced by membrane fluidity/potential, stress proteins, and the presence of sodium in the incubation medium (Endo et al, 1997;Braeckman et al, 1998). In addition, studies suggested a Refers to nonoccupationally exposed and/or low fish consumption.…”

mentioning

confidence: 95%

Toxicology and Immunotoxicology of Mercury: A Comparative Review in Fish and Humans

Sweet¹,

Zelikoff²

2001

Journal of Toxicology and Environmental Health Part B: Critical Reviews

206

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This review addresses an important area of environmental and mammalian toxicology by evaluating and comparing mercury-induced effects upon the immune responses of two evolutionarily divergent yet immunologically-related species. The mechanisms of mercury toxicology and immunotoxicology are described herein, including supporting data from the following: sources of exposure; bioavailability and biodistribution; metabolism; and laboratory and field investigations. Based upon the studies presented, the relative sensitivities of fish and human immune cells to mercury exposure are compared and contrasted with regard to mercury's ability to stimulate and/or suppress host immunocompetence. In addition, results from immune assays are compared to mercury tissue burdens, as well as to toxicological threshold level estimates. Such comparisons may help to resolve gaps in our knowledge regarding sensitivity of immunological assays, standardization of immunotoxicological techniques between species, and the extent to which the vertebrate immune system possesses functional reserve and redundancy in response to xenobiotics. A review of this type begins to provide support for the potential usefulness of fish immune cells to serve as indicators for human immunotoxicology risk assessment. Analysis of the reviewed studies supports the following conclusions in both lower and higher vertebrates: a threshold for mercury-induced immunotoxicological effects is likely; multiple exposure scenarios involving high and/or chronic exposures leading to increased body burdens are linked to increased risk of immunomodulation; and highly exposed and/or susceptible subpopulations are at greater risk of toxicological impact.

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Uptake of HgCl2and MeHgCl in an Insect Cell Line (Aedes albopictusC6/36)

Cited by 17 publications

References 29 publications

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

Bioaccumulation of inorganic and methylated mercury by the gills of the shore crab Carcinus maenas: transepithelial fluxes and histochemical localization

Cadmium Uptake and Defense Mechanism in Insect Cells

Toxicology and Immunotoxicology of Mercury: A Comparative Review in Fish and Humans

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