Uptake, Metabolism, and Effects on Detoxication Enzymes of Isoproturon in Spawn and Tadpoles of Amphibians

Greulich, Kerstin; Hoque, Enamul; Pflugmacher, Stephan

doi:10.1006/eesa.2002.2182

Cited by 28 publications

(20 citation statements)

References 34 publications

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“…heat shock, ozone, ethylene, heavy metals, and xenobiotic compounds), as well as by some inducers or chemoprotective substances (Manar et al 2004). Resultant glutathione conjugates are less toxic than the parent compounds and can be stored in vacuoles or excreted from the biological system, helping the organism regain normal cellular functions and reducing the cellular toxicity of reactive parent or organic intermediates (Greulich et al 2002). While examination of the kidney sections from prophylactic group showed moderate GST immunoreaction in the tubular cells, however, some cells showed strong reaction.…”

Section: Discussionmentioning

confidence: 99%

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Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

Fattah

He²,

Fa³

et al. 2010

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Abstract. This study aimed to investigate the possible protective role of curcumin against renal damage caused by administration of cyclosporine A (CsA) in adult male rats. For this purpose, 27 adult male albino rats were used and divided into three equal groups. Group I (control group) and group II (CsA-treated group) received a daily subcutaneous injection of CsA at a dose of 20 mg/kg b.w. Group III (prophylactic group) received a daily oral curcumin at a dose of 15 mg/kg b.w. simultaneously with CsA. After 21 days, all the animals were anaesthetized and the kidneys were rapidly removed and processed to prepare paraffin sections stained with H&E, PAS and Masson's trichrome. In addition, the glutathione S-transferase (GST) enzyme was detected immunohistochemically. The optical density and the area (in %) of positive GST immunoreactions were measured in the cytoplasm of renal tubules and glomeruli and the data were statistically analyzed. Examination of sections from CsA-treated group showed renal tubules with vacuolated cytoplasm and others with darkly stained pyknotic nuclei. Apical brush borders of proximal tubules were undefined and PAS positive granules were noticed in their cytoplasm. The renal corpuscles contained shrunken glomeruli with widening of their Bowman's spaces. Inflammatory cellular infiltrate and increase in the collagen fibers were observed between the renal tubules. In prophylactic group, the structure of renal tubules and corpuscles were preserved except few tubular darkly stained pyknotic nuclei. Numerous blood vessels, few cellular infiltration and thin collagen fibers were observed between the renal tubules. Statistical analysis of morphometric data showed significant increase in the optical density of GST immunoreactivity in the cells of renal tubules and glomeruli of CsAtreated group when compared with the control or prophylactic groups. However, a significant decrease in the area of GST immunoreactivity in sections from prophylactic group was observed when compared with control or CsA-treated groups. In conclusion, protective effect of curcumin against cyclosporine-induced nephrotoxicity in rats was proven based on the study of histological changes and GST immunoexpression. This study supposes the possible therapeutic applications of curcumin in CsA-treated patients.

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Section: Discussionmentioning

confidence: 99%

“…They can catalyze the conjugation of many electrophilic endogenous and xenobiotic compounds to glutathione (Park et al 2001). The resultant glutathione conjugates are less toxic than the parent compounds (Greulich et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

Fattah

He²,

Fa³

et al. 2010

gpb

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“…Much more cytosolic GST activity toward CDNB was detected than with the microsomal one in tadpole Bombina variegata, and the former activity showed a maximum at G stages 24 and 25, where the development of operculum and the disappearance of external gills were observed. 22) The authors also reported that the cytosolic GST activity at G stage 25 significantly increased by exposure to isoproturon as either its concentration or exposure period increased. A similar increase of GST activity in the same species was induced by cypermethrin.…”

Section: Glutathione-s-transferases (Gst)mentioning

confidence: 94%

“…22) Hydroxylation at the gem-methyl group of the cyclopropyl ring was reported for cypermethrin in adult R. temporaria.…”

Section: Metabolism Of Chemicals Including Pesticidesmentioning

confidence: 98%

“…22,23) When the spawn of R. temporaria within 3 days before hatching was exposed to DDT (log K ow =6.9) at 0.5 ppm for a day, no pesticide residue was detected in tadpoles hatching 21 days later, while the exposure of spawn 5 days before hatching resulted in significant residues (19.4 ppm) in the tadpoles with toxic signs and poor development. 24) This may imply the ability of a well-developed jelly coat to act as a barrier to pesticide exposure, but the possibility of less metabolic detoxification at an early developmental stage cannot be ruled out.…”

Section: Uptake and Depurationmentioning

confidence: 99%

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Bioconcentration and metabolism of pesticides and industrial chemicals in the frog

Katagi

Ose

2014

J. Pestic. Sci.

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Exposure to pesticide residues is claimed to be one of the possible causes of frog decline. Knowledge of basic information on the uptake, metabolism and depuration processes of pesticides in the frog is needed to understand the relationship between exposure and toxic effects from their actual body burden, together with their bioconcentration. The hydrophobicity of pesticides and industrial chemicals was one of the most important factors controlling bioconcentration, similarly to fish, when frogs are exposed to contaminated water. Skin absorption was also a key route in the uptake process especially in the adult frog. The metabolic profiles in the frog, mainly examined by an intraperitoneal injection technique, were common to other aquatic species without any frog-specific transformation reaction. The effects of developmental stage, sex, species and environmental factors such as temperature were observed for bioconcentration and metabolism.

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A Cloud Point Extraction Approach Developed for Analyzing Pesticides Prometryne and Isoproturon from Multi‐media

Tan

Jin

Yang

2013

CLEAN Soil Air Water

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A new analytic methodology based on the cloud point extraction coupled with HPLC (CPE‐HPLC) was developed and successfully applied to determination of the pesticides isoproturon (IPU) and prometryne (PRO) from multi‐media (contaminated water, soil, and food vegetable). Several non‐ionic surfactants including poly ethylene glycol 6000 (PEG‐6000), TritonX‐114, and Triton X‐100 were comparatively analyzed as extraction solvents. Other parameters such as surfactant concentration, ionic strength, and equilibration temperature, and duration were also investigated. The optimal conditions for CPE were presented with 2.4% w/v PEG‐6000, 11% w/v Na2SO4 and heating assistance at 50°C for 25 min. The calibration curves for the two analytes were linear ranging from 0.001 to 10.0 mg L−1, with correlation coefficients being 0.99 determined by a HPLC–UV detector. Under the condition, the average recoveries were 85.4–90.6% for water, 84.4–92.7% for soil, and 84.4–92.1% for vegetable. Thus, the method presented here was proved to be rapid, efficient, and green for extraction and determination of isoproturon and prometryne residues from food and multi‐environmental media.

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Uptake, Metabolism, and Effects on Detoxication Enzymes of Isoproturon in Spawn and Tadpoles of Amphibians

Cited by 28 publications

References 34 publications

Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

Prophylactic role of curcumin against cyclosporine-induced nephrotoxicity: Histological and immunohistological study

Bioconcentration and metabolism of pesticides and industrial chemicals in the frog

A Cloud Point Extraction Approach Developed for Analyzing Pesticides Prometryne and Isoproturon from Multi‐media

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