Uptake, disposition and depuration of domoic acid by blue mussels (Mytilus edulis)

Novaczek, Irené; Madhyastha, M. S.; Ablett, Richard F.; Johnson, Gerald R.; Nijjar, M. S.; Sims, David E.

doi:10.1016/0166-445x(91)90009-x

Cited by 50 publications

(28 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All P. multiseries clones were harvested at the stationary phase of growth, when cultures exhibited the maximum intra-cellular toxicity throughout the growth cycle. Only cellular toxicities are reported in Table 1, as particulate DA is known to be the primary route for toxin accumulation (Novaczek et al 1991). The clones were predominantly single-celled in the stationary phase, with < 5% of the cells forming short chains of ≤ 4 cells, such that chain-formation was not a confounding variable in these experiments.…”

Section: Methodsmentioning

confidence: 99%

Mechanisms contributing to low domoic acid uptake by oysters feeding on Pseudo-nitzschia cells. II. Selective rejection

Mafra¹,

Bricelj²,

Ward³

2009

Aquat. Biol.

View full text Add to dashboard Cite

Oysters accumulate relatively low levels of domoic acid (DA) compared to other bivalves. Mafra et al. (2009, in this Theme Section) identified feeding mechanisms of oysters that may lead to low DA accumulation during monospecific blooms of Pseudo-nitzschia multiseries. However, several different species of Pseudo-nitzschia, as well as other diatoms and flagellates, may co-occur during a bloom. Therefore, the present study investigates pre-ingestive feeding processes that operate when oysters Crassostrea virginica are exposed to mixed phytoplankton assemblages containing P. multiseries of varying cell length. Guided by video-endoscopy, material transported along the ventral and dorsal gill tracts was sampled and analyzed to determine the site for sorting of microalgae on the pallial organs. There was no preferential rejection of P. multiseries in pseudofeces when oysters were exposed to the alga in a mixed suspension with other diatom species (Thalassiosira weissflogii or Chaetoceros muelleri). In contrast, P. multiseries was preferentially rejected when mixed with the flagellates Isochrysis galbana or Rhodomonas lens, suggesting a qualitative mechanism for particle sorting. This occurred on the gills, followed by further selection on the palps. Oysters also preferentially rejected larger P. multiseries cells (82 to 90 µm) relative to smaller ones (24 to 28 µm) on the gills, while no further selection based on size occurred on the palps. This effect is attributed to the fact that P. multiseries cells with a length that exceeds the width of the principal filament aperture (ca. 68 µm) are more likely directed to the ventral tract and rejected in pseudofeces. These findings offer an additional explanation for the relatively low DA levels found in oysters during natural Pseudo-nitzschia spp. blooms.

show abstract

Section: Methodsmentioning

confidence: 99%

Mechanisms contributing to low domoic acid uptake by oysters feeding on Pseudo-nitzschia cells. II. Selective rejection

Mafra¹,

Bricelj²,

Ward³

2009

Aquat. Biol.

View full text Add to dashboard Cite

show abstract

“…Similarly, studies with the three species of gastropod (Lymnaea stagnalis, Helisoma trivolvis, and Physa gyrina) (Zurawell et al, 1999), three freshwater Unionid bivalves (A. Woodiana, C. plicata, and Unio dougiasiae) (Yokoyama and Park, 2002) indicated that the concentration of microcystins in the tissue of the invertebrates was correlated with the toxin in phytoplankton, but not with extracellular aqueous microcystins. Meanwhile, some studies with the freshwater clam, Anodonta grandis simpsoniana (Prepas et al, 1997), the blue mussel, Mytilus edulis (Novaczek et al, 1991), and the gastropods (Lance et al, 2006) pointed out that the primary routes of uptake of algal toxins are through diet, and to a lesser extent, via uptake of dissolved toxins. In the present study, PCCA indicates that among biological variables, Microcystis biomass showed closer positive relationships with intracellular and extracellular toxins content than with MCs content in snail hepatopancreas, suggesting that in addition to Microcystis, other factors (e.g., water temperature) also substantially affected the accumulation of MCs in snail hepatopancreas.…”

Section: Discussionmentioning

confidence: 99%

Spatial and temporal variations of microcystins in hepatopancreas of a freshwater snail from Lake Taihu

Zhang

Xie

Liu

et al. 2009

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

“…On the other hand, DA levels in mussels, the sentinel species that was used as an indicator of DA in coastal California, did not effectively predict the toxic potential of this bloom event. Past studies have indicated the difficulty in using mussels to detect toxic events due to their fast depuration of toxin (Novaczek et al 1991;Langlois et al 1993). During the spring 1998 episode, levels of DA in mussels reached 2.1 g DA g Ϫ1 tissue, whereas toxin levels in sardines and anchovies reached 257 g DA g Ϫ1 tissue (G. Langlois and S. Luscutoff, California Department of Health).…”

Section: Discussionmentioning

confidence: 99%

Domoic acid production near California coastal upwelling zones, June 1998

Trainer

Adams

Bill

et al. 2000

Limnology & Oceanography

179

149

View full text Add to dashboard Cite

Sea lion mortalities in central California during May and June 1998 were traced to their ingestion of sardines and anchovies that had accumulated the neurotoxin domoic acid. The detection of toxin in urine, feces, and stomach contents of several sea lions represents the first proven occurrence of domoic acid transfer through the food chain to a marine mammal. The pennate diatoms, Pseudo-nitzschia multiseries and P. australis, were the dominant, toxinproducing phytoplankton constituting algal blooms near Monterey Bay, Half Moon Bay, and Oceano Dunes, areas where sea lions with neurological symptoms stranded. Toxic Pseudo-nitzschia were also found near Morro Bay, Point Conception, Point Arguello, and Santa Barbara, demonstrating that these species were widespread along the central California coast in June 1998. Measurements of domoic acid during three cruises in early June showed the highest cellular toxin levels in P. multiseries near Point Año Nuevo at 6 pg cell Ϫ1 and in P. australis from Morro Bay at 78 pg cell Ϫ1 . Maximum cellular domoic acid levels were observed within 20 km of the coast between 0 and 5 m depth, although toxin was also measured to depths of 40 m. Hydrographic data indicated that the highest toxin levels and greatest numbers of toxic cells were positioned in water masses associated with upwelling zones near coastal headlands. Nutrient levels at these sites were less than those typically measured during periods of active upwelling, due to the 1998 El Niño event. The flow of cells and/or nutrients from coastal headlands into embayments where cells can multiply in a stratified environment is a possible mechanism of bloom development along the central California coast. This coupling of toxic Pseudo-nitzschia growth near upwelling zones with physical processes involved in cell transport will be understood only when long-term measurements are made at several key coastal locations, aiding in our capability to predict domoic-acid producing algal blooms.In May and June 1998, at least 70 California sea lions (Zalophus californianus) and one northern fur seal (Callorhinus ursinus) were found stranded along the central California coast. This stranding event occurred between 15 May 1 Corresponding author (Vera.L.Trainer@noaa.gov). AcknowledgmentsWe thank the captain and the crew of the RV Ballena, RV Shana Rae, and PB Bluefin for their help during these cruises, at times under strenuous conditions. We acknowledge the following people for their help in providing useful information that directed the locations of the cruises:

show abstract

Uptake, disposition and depuration of domoic acid by blue mussels (Mytilus edulis)

Cited by 50 publications

References 18 publications

Mechanisms contributing to low domoic acid uptake by oysters feeding on Pseudo-nitzschia cells. II. Selective rejection

Mechanisms contributing to low domoic acid uptake by oysters feeding on Pseudo-nitzschia cells. II. Selective rejection

Spatial and temporal variations of microcystins in hepatopancreas of a freshwater snail from Lake Taihu

Domoic acid production near California coastal upwelling zones, June 1998

Contact Info

Product

Resources

About