Uptake and transport of positron-emitting tracer (18F) in plants

Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil

“…Plant samples containing a positron-emitting nuclide were placed between two opposed 2D block detectors composed of Bi 4 Ge 3 O 12 scintillator arrays. Two annihilation ␥-rays from the decaying positrons were detected in coincidence by these detectors (24). The original position of the annihilation was localized at the intersection of the object plane with a line connecting the two detection points on the detectors.…”

Quantitative Modeling of Photoassimilate Flow in an Intact Plant Using the Positron Emitting Tracer Imaging System (PETIS)

“…It collects serial images of the two-dimensional distribution of a positron-emitting radiotracer in a test plant without contact (Kume et al 1997; Ishioka et al 1999; Uchida et al 2003). However, the advantage of PETIS is more than just capturing a movie for qualitative analysis.…”

Effects of anion channel blockers on xylem nitrate transport in barley seedlings

“…PETIS has been shown to be a useful tool for the detection of two-dimensional and real-time distribution of uN by measuring the amount of )I-rays emitted from positrons without destruction of the plants (Hayashi et al 1997;Kume et al 1997;Sato et al 1999;Kiyomiya et al 2001;Ohtake et al 2001). Since NR activities in both shoots and roots were undetectable for the first several hours when nitrate-starved barley plants were exposed to the medium containing nitrate (Somers et al 1983;Melzer et al 1989), the 13N accumulated in the shoots observed in this experiment consisted mostly of i3NO, -which is transported from roots.…”

“…For the measurement of the nitrate flux in intact plants, I3NO]-and 15N03 -were used as tracers. Positron Emitting Tracer Imaging Systems (PETIS) could detect the two-dimensional and real-time distribution of 13N by measuring the amount of 'Y-rays emitted from positrons without destruction of the plant (Hayashi et al 1997;Kume et al 1997;Sato et al 1999;Kiyomiya et al 2001;Ohtake et al 2001). The net nitrate uptake was measured accurately by emission spectrometry using 15N03 -.…”