Quantitative Modeling of Photoassimilate Flow in an Intact Plant Using the Positron Emitting Tracer Imaging System (PETIS)

Matsuhashi, Sachiko; Fujimaki, Shu; Kawachi, Naoki; Sakamoto, Koichi; Ishioka, Noriko S.; Kume, Tamikazu

doi:10.1111/j.1747-0765.2005.tb00047.x

Cited by 30 publications

(25 citation statements)

References 8 publications

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“…Carbon older than 2-3 days contributed little to new tissue growth, and shaded plants tended to grow more slowly, further reinforcing the close linkage between photosynthesis and growth rate. Short-term labelling studies show that photoassimilates can travel 2-3 cm per minute in Vicia faba L. [98], potentially allowing C to reach leaf growth zones within 10 to 20 min from assimilation, and reinforcing the close linkage between photosynthesis and growth. Similarly to new tissues, when we look at root exudates, we find that approximately 58% of the C in L. perenne root exudates derives from recent assimilation [99].…”

Section: Carbon Allocationmentioning

confidence: 99%

Carbon Assimilation, Biomass Partitioning and Productivity in Grasses

Irving

2015

Agriculture

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Plant growth correlates with net carbon gain on a whole plant basis. Over the last several decades, the driving factors shaping plant morphology and performance have become increasingly clear. This review seeks to explore the importance of these factors for grass performance. Briefly, these fall into factors influencing photosynthetic rates directly, competition between plants in a canopy, and nutrient status and availability.

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Section: Carbon Allocationmentioning

confidence: 99%

Carbon Assimilation, Biomass Partitioning and Productivity in Grasses

Irving

2015

Agriculture

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“…Furthermore, the time course of tracer amounts within any selected region of interest (ROI) on the obtained image can be easily generated and applied for mathematical analyses because PETIS provides highly quantitative images. The rates of photoassimilation and the velocities of phloem transport in intact plants under various environmental conditions have been estimated quantitatively using PETIS (Matsuhashi et al, 2005;Kawachi et al, 2006). However, to our knowledge, no study has been carried out to describe the whole dynamics and kinetics of a substance in an intact plant body by taking full advantage of PETIS, namely noninvasive visualization and quantitative time-course analysis.…”

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confidence: 99%

Tracing Cadmium from Culture to Spikelet: Noninvasive Imaging and Quantitative Characterization of Absorption, Transport, and Accumulation of Cadmium in an Intact Rice Plant

et al. 2010

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We characterized the absorption and short-term translocation of cadmium (Cd) in rice (Oryza sativa 'Nipponbare') quantitatively using serial images observed with a positron-emitting tracer imaging system. We fed a positron-emitting 107 Cd (halflife of 6.5 h) tracer to the hydroponic culture solution and noninvasively obtained serial images of Cd distribution in intact rice plants at the vegetative stage and at the grain-filling stage every 4 min for 36 h. The rates of absorption of Cd by the root were proportional to Cd concentrations in the culture solution within the tested range of 0.05 to 100 nM. It was estimated that the radial transport from the culture to the xylem in the root tissue was completed in less than 10 min. Cd moved up through the shoot organs with velocities of a few centimeters per hour at both stages, which was obviously slower than the bulk flow in the xylem. Finally, Cd arrived at the panicles 7 h after feeding and accumulated there constantly, although no Cd was observed in the leaf blades within the initial 36 h. The nodes exhibited the most intensive Cd accumulation in the shoot at both stages, and Cd transport from the basal nodes to crown root tips was observed at the vegetative stage. We conclude that the nodes are the central organ where xylem-to-phloem transfer takes place and play a pivotal role in the half-day travel of Cd from the soil to the grains at the grain-filling stage.

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“…Various important compounds that contain positron-emitting nuclides (e.g., major nutrients, water, amino acids, glucose, minor elements, and environmental pollutants) can be synthesized routinely and safely after bombardment of the target elements with the accelerated beam from a cyclotron (Ishioka et al, 1999;Kume et al, 1997). By using PETIS and 11 C, it recently became possible to observe the translocation of photosynthate in intact plants quantitatively and noninvasively (Kawachi et al, 2006;Matsuhashi et al, 2005).…”

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Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System

Kikuchi

Ishii

Fujimaki

et al. 2008

J. Japan. Soc. Hort. Sci.

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A recently developed positron-emitting tracer imaging system (PETIS) noninvasively produces quantitative realtime images of the movement of various compounds in plants. To clarify the mechanism of the growth and development of the fruit of eggplant (Solanum melongena L.), we fed 11 CO 2 to a leaf and monitored the translocation of 11 C-labeled photoassimilate into the fruit by PETIS. Continuous images of the translocation of [11 C]photoassimilate from the leaf to the fruit and inside the fruit were produced, with a shorter time resolution than reported previously. 11 C signal intensity in the fruit increased gradually, and its distribution was not uniform. The fruits were sliced after PETIS measurement and exposed to the imaging plates of a bio-imaging analyzer system. The resultant images showed the localization of 11 C activity inside the fruit, and indicated that translocation of photoassimilate within the fruit was not uniform. The velocity of photoassimilate translocation in the peduncle and changes in the rate of translocation of photoassimilate in the fruit were estimated by analysis of PETIS data. The velocity of photoassimilate translocation through the peduncle was estimated as 1.17 cm·min −1. About 60 min after the start of 11 CO 2 feeding, the 11 C activity of the fruit began to increase, and by 120 min it had reached about 8% of feeding 11 CO 2 . These results indicate that it took about 60 min for the first [11 C]photoassimilates to reach the fruit. Real time observation of photoassimilate translocation within a fruit has never been reported. PETIS may be a powerful tool for revealing the mechanisms of fruit development and maturity.

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Quantitative Modeling of Photoassimilate Flow in an Intact Plant Using the Positron Emitting Tracer Imaging System (PETIS)

Cited by 30 publications

References 8 publications

Carbon Assimilation, Biomass Partitioning and Productivity in Grasses

Carbon Assimilation, Biomass Partitioning and Productivity in Grasses

Tracing Cadmium from Culture to Spikelet: Noninvasive Imaging and Quantitative Characterization of Absorption, Transport, and Accumulation of Cadmium in an Intact Rice Plant

Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System

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