Upstream regulatory regions from the maize Sh1 promoter confer tissue-specific expression of the , -glucuronidase gene in tomato

D’Aoust, Marc‐André; Nguyen‐Quoc, Binh; Le, Van Quy; Yelle, Serge

doi:10.1007/s002990050665

Cited by 11 publications

(5 citation statements)

References 21 publications

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“…3b). This observed difference in tissue specificity might be attributed to post-transcriptional regulation of GUS expression, as has been previously reported (D'Aoust et al, 1999). Therefore, whether we were dealing with stable or transient expression of a reporter or any other useful gene, expression levels of the gene of interest were significantly enhanced when the untranslated leader sequence of AMV RNA4 was added to the chimeric gene construct.…”

Section: Resultsmentioning

confidence: 70%

Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

Krasnyanski

Sandhu

Domier

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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Two different promoters, a cauliflower mosaic virus (CaMV) 35S promoter with a 5 H -untranslated leader sequence from alfalfa mosaic virus RNA4 (designated as CaMV 35S/AMV) and an E-8 fruit-ripening-specific promoter, were compared to evaluate their effects on expression of the uidA reporter gene in transgenic tomato plants. In order to generate sufficient numbers of transgenic tomato plants, both a reliable regeneration system and an efficient Agrobacterium transformation protocol were developed using 8-d-old cotyledons of tomato (Lycopersicon ecsulentum Mill. cv. Swifty Belle). Two sets of constructs, both derivatives of the binary vector pBI121, were used in transformation of tomato whereby the uidA gene was driven either by the CaMV 35S/AMV or the E-8 fruit-ripening-specific promoter. Southern blot hybridization confirmed the stable integration of the chimeric uidA gene into the tomato genome. Fruit and leaf tissues were collected from T 0 and T 1 plants, and assayed for b-glucuronidase (GUS) enzyme activity. As expected, both vegetative and fruit tissues of transgenic plants carrying the uidA gene under the control of CaMV 35S/AMV showed varying levels of GUS activity, while no expression was observed in vegetative tissues of transgenic plants carrying the uidA gene driven by the E-8 promoter. All fruits from transgenic plants produced with both sets of constructs displayed expression of the uidA gene. However, when this reporter gene was driven by the CaMV 35S/AMV, GUS activity levels were significantly higher than when it was driven by the E-8 fruit-specific promoter. The presence/absence of the uidA gene in T 1 plants segregated in a 3:1 Mendelian ratio.

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Section: Resultsmentioning

confidence: 70%

Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

Krasnyanski

Sandhu

Domier

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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“…Tomato plants were transformed with each of the above silencing or overexpression constructs according to D'Aoust et al (1999). Briefly, seeds were germinated in half-strength MS medium.…”

Section: Gene Constructs and Plant Transformationmentioning

confidence: 99%

Posttranslational Elevation of Cell Wall Invertase Activity by Silencing Its Inhibitor in Tomato Delays Leaf Senescence and Increases Seed Weight and Fruit Hexose Level

2009

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Invertase plays multiple pivotal roles in plant development. Thus, its activity must be tightly regulated in vivo. Emerging evidence suggests that a group of small proteins that inhibit invertase activity in vitro appears to exist in a wide variety of plants. However, little is known regarding their roles in planta. Here, we examined the function of INVINH1, a putative invertase inhibitor, in tomato (Solanum lycopersicum). Expression of a INVINH1:green fluorescent protein fusion revealed its apoplasmic localization. Ectopic overexpression of INVINH1 in Arabidopsis thaliana specifically reduced cell wall invertase activity. By contrast, silencing its expression in tomato significantly increased the activity of cell wall invertase without altering activities of cytoplasmic and vacuolar invertases. Elevation of cell wall invertase activity in RNA interference transgenic tomato led to (1) a prolonged leaf life span involving in a blockage of abscisic acid-induced senescence and (2) an increase in seed weight and fruit hexose level, which is likely achieved through enhanced sucrose hydrolysis in the apoplasm of the fruit vasculature. This assertion is based on (1) coexpression of INVINH1 and a fruit-specific cell wall invertase Lin5 in phloem parenchyma cells of young fruit, including the placenta regions connecting developing seeds; (2) a physical interaction between INVINH1 and Lin5 in vivo; and (3) a symplasmic discontinuity at the interface between placenta and seeds. Together, the results demonstrate that INVINH1 encodes a protein that specifically inhibits the activity of cell wall invertase and regulates leaf senescence and seed and fruit development in tomato by limiting the invertase activity in planta.

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“…Although the 35S promoter directs a high level of expression in the endosperm and embryo tissues of tomato seeds (D'Aoust et al, 1999), expression of an antisense RNA from TOMSSF only slightly inhibited SuSy in the endosperm and did not reduce SuSy activity in the embryo (Table 1). In transgenic lines A48 and A45, which exhibited as much as 98 and 90% SuSy inhibition, respectively, the respective reduction of SuSy activity in the endosperm tissue was only 33 and 20%.…”

Section: Production Of Tomato Plants With Reduced Fruit Susy Activitymentioning

confidence: 99%

“…Tomato plants were transformed essentially as described previously (D'Aoust et al, 1999). Briefly, well-expanded cotyledons of 10-dayold seedlings were excised and placed upside down on the induction medium.…”

Section: Plant Transformationmentioning

confidence: 99%

Antisense Inhibition of Tomato Fruit Sucrose Synthase Decreases Fruit Setting and the Sucrose Unloading Capacity of Young Fruit

1999

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The role of sucrose synthase (SuSy) in tomato fruit was studied in transgenic tomato ( Lycopersicon esculentum ) plants expressing an antisense fragment of fruit-specific SuSy RNA ( TOMSSF ) under the control of the cauliflower mosaic virus 35S promoter. Constitutive expression of the antisense RNA markedly inhibited SuSy activity in flowers and fruit pericarp tissues. However, inhibition was only slight in the endosperm and was undetectable in the embryo, shoot, petiole, and leaf tissues. The activity of sucrose phosphate synthase decreased in parallel with that of SuSy, but acid invertase activity did not increase in response to the reduced SuSy activity. The only effect on the carbohydrate content of young fruit was a slight reduction in starch accumulation. The in vitro sucrose import capacity of fruits was not reduced by SuSy inhibition at 23 days after anthesis, and the rate of starch synthesized from the imported sucrose was not lessened even when SuSy activity was decreased by 98%. However, the sucrose unloading capacity of 7-day-old fruit was substantially decreased in lines with low SuSy activity. In addition, the SuSy antisense fruit from the first week of flowering had a slower growth rate. A reduced fruit set, leading to markedly less fruit per plant at maturity, was observed for the plants with the least SuSy activity. These results suggest that SuSy participates in the control of sucrose import capacity of young tomato fruit, which is a determinant for fruit set and development.

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Upstream regulatory regions from the maize Sh1 promoter confer tissue-specific expression of the , -glucuronidase gene in tomato

Cited by 11 publications

References 21 publications

Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

Posttranslational Elevation of Cell Wall Invertase Activity by Silencing Its Inhibitor in Tomato Delays Leaf Senescence and Increases Seed Weight and Fruit Hexose Level

Antisense Inhibition of Tomato Fruit Sucrose Synthase Decreases Fruit Setting and the Sucrose Unloading Capacity of Young Fruit

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