Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

Krasnyanski, Sergei F.; Sandhu, Jagdeep Singh; Domier, Leslie L.; Buetow, D.E.; Korban, Schuyler S.

doi:10.1007/s11627-001-0075-1

Cited by 33 publications

(18 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KRASNYANSKI et al (2001) also studied the influence of TDZ on shoot organogenesis of cotyledon explants. In their experiment the mean number of shoots per explant was significantly increased by using the combination of BAP and TDZ, and by elevating the concentration of TDZ from 2.27 µM to 4.54 µM.…”

Section: Discussionmentioning

confidence: 99%

Influence of growth regulators on plant regeneration in tomato

Gubiš¹,

Lajchová²,

Klčová³

et al. 2005

Hort. Sci. (Prague)

View full text Add to dashboard Cite

ABSTRACT:We studied the effect of different plant growth regulators on in vitro regeneration and plant growth of three cultivars of tomato (Lycopersicon esculentum Mill.) from explants derived from hypocotyls and cotyledons of aseptically grown seedlings. The regeneration capacity was significantly influenced by cultivar and explant type. The highest number of shoots regenerated in both types of explants was recorded on MS medium supplemented with 1.0 mg/dm 3 zeatin and 0.1 mg/dm 3 IAA. The cultivar UC 82 showed the best regeneration capacity on all types of used media. The most responsive explants were hypocotyls with 90-92% regeneration in dependence on the used cultivars and mean production from 0.18 to 0.38 shoots per explant. 119of MURASHIGE and SKOOG (1962) (hereinafter abbreviated as MS), 100 mg/dm 3 myo-inositol, 2 mg/dm 3 thiamine.HCl, 0.5 mg/dm 3 pyridoxine. HCl, 0.5 mg/dm 3 nicotinic acid, 1% (w/v) sucrose and 0.7% (w/v) agar. The cultures were initially kept in the dark at 27 ± 1°C for two days and then maintained under a 16 h photoperiod at 50 µmol m 2 s, with day/night temperature of 25°C/20°C. Hypocotyl and cotyledon segments were cut from the seedlings grown in vitro. The hypocotyls were cut into three segments. Each cotyledon was transversally cut into two segments. Hypocotyls were transversally cut into 4-7 mm segments and leaf-blades into pieces of 30-40 mm 2 . The hypocotyl explants were placed horizontally on the medium surface and cotyledon explants with the adaxial surface in contact with the medium. Regeneration was induced on MS medium supplemented with different concentrations of cytokinins (ZEA, BAP, TDZ) and auxin (IAA) (Table 1). After 3 weeks cultured explants were transferred on: 1) MS without plant growth regulators (PGRs), 2) MS medium contains half concentration of PGRs and 3) MS medium with full concentration of PGRs (Fig. 1). The media were adjusted to pH 5.8 prior to autoclaving. Glass containers with 25 cm 3 of medium were used.Six weeks later the regeneration capacity of explants was assessed. The following parameters were evaluated: the frequency of regeneration (percent of regenerating explants) and the number of shoots per explant. Data on regeneration frequency (%) were transformed by arcsin√x prior to statistical analysis. The experiment was repeated twice using 30 explants per variant. Significance of difference between the results was estimated by analysis of variance (ANOVA). Variation among means was analysed using LSD (P ≤ 0.05) procedure. RESULTSThe effect of different tomato cultivars and different PGRs concentrations on shoot regeneration from aseptically grown hypocotyls and cotyledons showed significant variation in both the genotype and PGRs. A large variability in the number of shoots was observed between cultivars and between the different PGRs concentrations (Table 2). Cultivar mean comparisons (least significant difference, LSD, P ≤ 0.05) showed only two classes differing in the induction potential. The cultivar which produced the most shoots on hypocot...

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of growth regulators on plant regeneration in tomato

Gubiš¹,

Lajchová²,

Klčová³

et al. 2005

Hort. Sci. (Prague)

View full text Add to dashboard Cite

show abstract

“…The sense primer contained a 37-bp untranslated region sequence from Alfalfa mosaic virus upstream of the SlBRI1 start sequence that has been shown previously to enhance the expression of transgenes in transformed tomato plants (Krasnyanski et al, 2001). The PCR product was gel purified, digested with SmaI and SacI, and introduced into the binary vector pBI121 (Clontech) that had also been digested with SmaI/SacI and gel purified to remove the uidA gene, to yield the plasmid (35S:SlBRI1-Flag/pBI121).…”

Section: Cloning Of Slbri1-flag Into Plant Transformation Vectorsmentioning

confidence: 99%

“…Agrobacterium tumefaciens-mediated transformation was performed essentially as described by Krasnyanski et al (2001) with modifications. Seeds of the weak allele of SlBRI1, cu3 -abs (Montoya et al, 2002), were sterilized with 70% …”

mentioning

confidence: 99%

Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites

Bajwa¹,

Blackburn

Goshe

et al. 2013

Plant Physiology

View full text Add to dashboard Cite

Brassinosteroids (BRs) are plant hormones that are perceived at the cell surface by a membrane-bound receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1). BRI1 interacts with BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) to initiate a signal transduction pathway in which autophosphorylation and transphosphorylation of BRI1 and BAK1, as well as phosphorylation of multiple downstream substrates, play critical roles. Detailed mechanisms of BR signaling have been examined in Arabidopsis (Arabidopsis thaliana), but the role of BRI1 and BAK1 phosphorylation in crop plants is unknown. As a foundation for understanding the mechanism of BR signaling in tomato (Solanum lycopersicum), we used liquid chromatography-tandem mass spectrometry to identify multiple in vitro phosphorylation sites of the tomato BRI1 and BAK1 cytoplasmic domains. Kinase assays showed that both tomato BRI1 and BAK1 are active in autophosphorylation as well as transphosphorylation of each other and specific peptide substrates with a defined sequence motif. Site-directed mutagenesis revealed that the highly conserved kinase domain activation loop residue threonine-1054 was essential for tomato BRI1 autophosphorylation and peptide substrate phosphorylation in vitro. Furthermore, analysis of transgenic lines expressing full-length tomato BRI1-Flag constructs in the weak tomato bri1 allele, curl3 -abs1 , demonstrated that threonine-1054 is also essential for normal BRI1 signaling and tomato growth in planta. Finally, we cloned the tomato ortholog of TGF-b Receptor Interacting Protein (TRIP1), which was previously shown to be a BRI1-interacting protein and kinase domain substrate in Arabidopsis, and found that tomato TRIP1 is a substrate of both tomato BRI1 and BAK1 kinases in vitro.Brassinosteroids (BRs) are regulators of plant growth and development that signal through the membranebound receptor kinase BRASSINOSTEROID INSEN-STIVE1 (BRI1) and its coreceptor BRI1-ASSOCIATED

show abstract

“…In tomato, E8 is regulated by ethylene and is activated at the onset of fruit ripening (Peñarrubia et al, 1992;Kneissl and Deikman, 1996). The promoter of E8 has been characterized and is widely used to drive the expression of exogenous genes in transgenic tomato fruits (Sandhu et al, 2000;Krasnyanski et al, 2001;Kesanakurti et al, 2012).…”

mentioning

confidence: 99%

A Tomato MADS-Box Transcription Factor, SlMADS1, Acts as a Negative Regulator of Fruit Ripening

et al. 2013

View full text Add to dashboard Cite

box genes encode a highly conserved gene family of transcriptional factors that regulate numerous developmental processes in plants. In this study, a tomato (Solanum lycopersicum) MADS-box gene, SlMADS1, was cloned and its tissue-specific expression profile was analyzed. The real-time polymerase chain reaction results showed that SlMADS1 was highly expressed in sepals and fruits; its expression level was increased with the development of sepals, while the transcript of SlMADS1 decreased significantly in accordance with fruit ripening. To further explore the function of SlMADS1, an RNA interference (RNAi) expression vector targeting SlMADS1 was constructed and transformed into tomato plants. Shorter ripening time of fruit was observed in SlMADS1-silenced tomatoes. The accumulation of carotenoid and the expression of PHYTOENE SYNTHETASE1 were enhanced in RNAi fruits. Besides, ethylene biosynthetic genes, including 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE1A, 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE6, 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE1, and 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE3, and the ethylene-responsive genes E4 and E8, which were involved in fruit ripening, were also up-regulated in silenced plants. SlMADS1 RNAi fruits showed approximately 2-to 4-fold increases in ethylene production compared with the wild type. Furthermore, SlMADS1-silenced seedlings displayed shorter hypocotyls and were more sensitive to 1-aminocyclopropane-1-carboxylate than the wild type. Additionally, a yeast two-hybrid assay revealed a clear interaction between SlMADS1 and SlMADS-RIN. These results suggest that SlMADS1 plays an important role in fruit ripening as a repressive modulator.

show abstract

Effect of an enhanced CaMV 35S promoter and a fruit-specific promoter on uida gene expression in transgenic tomato plants

Cited by 33 publications

References 33 publications

Influence of growth regulators on plant regeneration in tomato

Influence of growth regulators on plant regeneration in tomato

Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites

A Tomato MADS-Box Transcription Factor, SlMADS1, Acts as a Negative Regulator of Fruit Ripening

Contact Info

Product

Resources

About