Upregulation of Vascular Endothelial Growth Factor Expression in the Kidney Could Be Reversed Following Treadmill Exercise Training in Type I Diabetic Rats

Al-Jarrah,

doi:10.14740/wjnu153e

Cited by 9 publications

(11 citation statements)

References 35 publications

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“…Furthermore, moderate-intensity exercise has been reported to reduce advanced glycation end products and ameliorate early diabetic nephropathy in obese Zucker rats 35 . Therefore, we agree with the conclusion that exercise training may reduce VEGF upregulation in diabetic kidneys by reducing hyperglycemia and increasing insulin sensitivity 34 . Similar favorable alterations occurred in kidney tissue in response to exercise performed at various frequencies.…”

Section: Discussionsupporting

confidence: 90%

“…VEGF upregulation has been reported in diabetic kidney tissue similar to our study 5 . In a study conducted by Al-Jarrah et al 34 rats were given treadmill exercise at a speed of 18 m/min 40 min/day for 5 days/week and diabetes-related elevation of VEGF levels declined. In our study, VEGF expression declined in the exercise groups, in line with the literature.…”

Section: Discussionmentioning

confidence: 99%

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Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Alaca¹,

Uslu²,

Başdemir³

et al. 2019

MMJ

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Objective: Exercise has many beneficial effects in the treatment and prevention of Type 2 Diabetes Mellitus (T2DM). The aim of this study was to evaluate the effect of physical activities with different frequencies performed within a total total duration of one week on the heart and kidney tissues and vascular endothelial growth factor (VEGF) expressions in experimental T2DM model. Method: Rats (n: 30) were divided into sedentary control (SC), sedentary T2DM (SD), T2DM and continuous exercise (DEc, 30 min/day, 5 days/week), T2DM and short bouts exercise (DEsb, 3x10 min/day, 5 days/week), T2DM and weekend warrior exercise (DEww, 35+40 min/day, 2 days/week) groups. Rats were administered streptozotocin (65 mg/kg) and nicotinamide (110 mg/kg) through intraperitoneal route. After 6-weeks of swimming exercise (total duration 150 min/week), biochemical analyzes were performed to measure oral glucose tolerance test, insulin sensitivity and cytokines. Histopathological and immunohistochemical analyses [VEGF, capillary density, Transforming growth factor beta (TGF-β)] were performed in heart and kidney tissues. Results: Compared with sedentary T2DM rats, significant improvements were observed in all exercise groups in terms of blood glucose level, insulin sensitivity, capillary density in heart tissue, VEGF expressions in tissues, TGF-β expressions in kidney tissue and all histopathological analysis (p<0.05). Conclusion: This study shows that physical activity at various frequencies may significantly ameliorate harmful effects of T2DM on heart and kidney tissue without significant differences between exercise frequencies, provided that the total duration of aerobic exercise remains the same (150 min/week).

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Alaca¹,

Uslu²,

Başdemir³

et al. 2019

MMJ

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“…Immunohistochemistry of IL-1β, TNF-α, and NF kappa B p65 was performed using a previously described protocol [25,28–32]. Briefly, deparaffinization and rehydration were performed in the prepared 4-μm-thick paraffin-embedded gastrocnemius skeletal muscle sections.…”

Section: Methodsmentioning

confidence: 99%

“…A digital camera was used to photograph 10 random regions of each section that were subsequently examined for IL-1β expression, TNF-α expression, and nuclear localization of NF kappa B in the gastrocnemius skeletal muscle. Then, total pixels region of positive staining (Figures 1C, 2C, 3C) was computed in proportion to the entire pixels region in each photographed area of each section using Adobe Photoshop software, as described previously [25,28–32]. After that, the mean of the pixels region of positive immunostaining relative to the entire pixels region was computed for every animal in the 2 different groups.…”

Section: Methodsmentioning

confidence: 99%

Interleukin-1 Beta and Tumor Necrosis Factor Alpha Upregulation and Nuclear Factor Kappa B Activation in Skeletal Muscle from a Mouse Model of Chronic/Progressive Parkinson Disease

Erekat

Al-Jarrah

2018

Med Sci Monit

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Background Skeletal muscle atrophy has been reported in patients with Parkinson disease (PD). The purpose of this study was to examine the potential implication of interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and nuclear factor kappa B (NF kappa B) in skeletal muscle atrophy following PD induction. Material/Methods Chronic Parkinsonism was induced in 10 albino mice by MPTP/probenecid treatment, while 10 other albino mice remained without treatment and were subsequently used as controls. Gastrocnemius muscles were examined for the expression of IL-1β and TNF-α, as well as the nuclear localization of NF kappa B, indicative of its activation, using immunohistochemistry in the 2 different groups. Results IL-1β and TNF-α expression and NF kappa B nuclear localization were significantly higher in the PD skeletal muscle compared with those in the controls (P value <0.01). Conclusions The present data are indicative of an association of PD with IL-1β and TNF-α upregulation and NF kappa B activation in gastrocnemius muscles, potentially promoting the atrophy frequently observed in PD.

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“…Then, serial longitudinal 4-μm–thick paraffin-embedded sections were cut. The sections were processed via immunohistochemistry according to previously described protocols [ 28 , 30 – 36 ]. To summarize, the sections were firstly deparaffinized and rehydrated.…”

Section: Methodsmentioning

confidence: 99%

Association of Parkinson Disease Induction with Cardiac Upregulation of Apoptotic Mediators P53 and Active Caspase-3: An Immunohistochemistry Study

Erekat

Al-Jarrah

2018

Med Sci Monit Basic Res

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BackgroundApoptosis plays a key role in the pathogenesis of Parkinson disease (PD). Active caspase-3, which is a proapoptotic factor, has been shown to reduce cardiac contractility, causing cardiac dysfunction in many pathological diseases. Reduced cardiac contractility and cardiac autonomic dysfunction have been reported in PD patients and PD mice treated with MPTP. The aim of this study was to show the impact of PD induction on the expression of the apoptotic mediators p53 and active caspase-3 in the heart.Material/MethodsEqual control and PD groups were formed by 20 randomly selected normal albino mice. We used 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (25 mg/kg) and probenecid (250 mg/kg) (MPTP/p) to induce chronic Parkinsonism in the PD group. Immunohistochemistry was performed to investigate the expression of p53, active caspase-3, and β-adrenergic receptor in hearts from the 2 animal groups.ResultsP53 and active caspase-3 expression was significantly higher in PD hearts than in the control hearts (p value <0.01). β-adrenergic receptor expression was significantly lower in PD hearts than in control hearts (p value <0.01).ConclusionsOur results show an association of PD with p53 and active caspase-3 overexpression and β-adrenergic receptor underexpression in the heart, potentially promoting the cardiac autonomic dysfunction frequently observed in PD.

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Upregulation of Vascular Endothelial Growth Factor Expression in the Kidney Could Be Reversed Following Treadmill Exercise Training in Type I Diabetic Rats

Cited by 9 publications

References 35 publications

Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Effects of three different frequencies of aerobic physical activity on heart and kidney tissues in type 2 diabetes-induced rats

Interleukin-1 Beta and Tumor Necrosis Factor Alpha Upregulation and Nuclear Factor Kappa B Activation in Skeletal Muscle from a Mouse Model of Chronic/Progressive Parkinson Disease

Association of Parkinson Disease Induction with Cardiac Upregulation of Apoptotic Mediators P53 and Active Caspase-3: An Immunohistochemistry Study

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