Upregulation of matrix metalloproteinase‐1 production by prostaglandin F_2α in human gingival fibroblasts

Abstract

In the present study, we investigated the effect of prostglandin F2alpha (PGF2alpha) and combination of PGF2alpha and interleukin(IL)-1beta on matrix metalloproteinase (MMP)-1 production in human gingival fibroblasts (HGF). PGF2alpha enhanced MMP-1 production in a dose-dependent manner in HGF. Combination of PGF2alpha and IL-1beta induced a synergistic increase of MMP-1 production in HGF. Furthermore, fluprostenol, a specific FP receptor agonist, increased MMP-1 production and induced a synergistic enhancement of IL-1beta-induced MMP-1 production in HGF, similar to PGF2alpha. FP receptor mRNA expression was detected in HGF, by reverse transcription-polymerase chain reaction. Northern blot analysis revealed that PGF2alpha enhanced MMP-1 mRNA expression in HGF and that PGF2alpha increased MMP-1 mRNA levels induced by IL-1beta. In conclusion, we suggest that PGF2alpha increases MMP-1 production in HGF and synergistically enhances MMP-1 production in IL-1beta-stimulated HGF. PGF2alpha may be involved in degradation of connective tissue in periodontal lesions.

Prostaglandin F2α-Induced Interleukin-8 Production in Human Dental Pulp Cells Is Associated With MEK/ERK Signaling

“…PGF 2a has been shown to induce the differentiation of cementoblasts and stimulate the matrix metalloproteinase activity as well as IL-6 production of gingival fibroblasts (10,11,31). In this study, exposure to PGF 2a stimulates IL-8 production of pulp cells.…”

“…Prostaglandin F 2alpha (PGF 2a ) is one of the PG family members that has diverse functions such as mitogenic to fibroblasts and osteoblasts as well as the induction of bone resorption (10,11). PGF 2a might also affect the pathobiologic processes of tissue inflammation and cardiovascular and rheumatic diseases (12).…”

Phenytoin Effects on Proliferation and Induction of IL1<i>β</i> and PGE2 in Pediatric and Adults’ Gingival Fibroblasts

“…This cytokine probably causes a decrease in the expression of procollagen and an increase in expression of procollagenase, and so could be known as a stimulator for destroying collagen. Noguchi [47] showed that at the presence of phenytoin, the number of IL-1 β receptors increase, by the way, the IL-1β which has been produced by local immune cells has more paracrine attachment.…”

Prostaglandin F2α stimulates MEK‐ERK signalling but decreases the expression of alkaline phosphatase in dental pulp cells

“…Interestingly, the FP receptor mRNA expression in GF, buccal mucosa fibroblasts and MG63 cells was identified, but not in GK, SAS tongue cancer epithelial cells and Eahy 926 endothelial cells. Prior reports have detected the expression of FP receptor in GF and MG63 osteoblasts (Noguchi et al. 2001b, Samadfam et al.…”