Upregulation of KIF20A correlates with poor prognosis in gastric cancer

Sheng, Yi; Wang, Wei; Hong, Bo; Jiang, Xingwang; Sun, Ruochuan; Yan, Qiang; Zhang, Shangxin; Lu, Mingdian; Wang, Shengyi; Zhang, Zhen; Lin, Wenchu; Li, Yongxiang

doi:10.2147/cmar.s176147

Cited by 39 publications

(30 citation statements)

References 39 publications

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“…KIF4A, KIF14 and KIF20A are shown to be overexpressed across many human cancer types suggesting a link between expression levels and overall survival. Furthermore, their knockdown inhibited proliferation and migration in breast, prostate, lung, ovarian and gastric tumors [73,74,75,76,77,78,79,80,81,82,83,84,85]. In meningiomas, however, KIF20A demonstrated an effect on progression-free survival but failed to show a significant functional impact on cell proliferation in vitro.…”

Section: Discussionmentioning

confidence: 99%

Identification of KIF11 As a Novel Target in Meningioma

Jungwirth

Tao

Moustafa

et al. 2019

Cancers

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Kinesins play an important role in many physiological functions including intracellular vesicle transport and mitosis. The emerging role of kinesins in different cancers led us to investigate the expression and functional role of kinesins in meningioma. Therefore, we re-analyzed our previous microarray dataset of benign, atypical, and anaplastic meningiomas (n = 62) and got evidence for differential expression of five kinesins (KIFC1, KIF4A, KIF11, KIF14 and KIF20A). Further validation in an extended study sample (n = 208) revealed a significant upregulation of these genes in WHO°I to °III meningiomas (WHO°I n = 61, WHO°II n = 88, and WHO°III n = 59), which was most pronounced in clinically more aggressive tumors of the same WHO grade. Immunohistochemical staining confirmed a WHO grade-associated upregulated protein expression in meningioma tissues. Furthermore, high mRNA expression levels of KIFC1, KIF11, KIF14 and KIF20A were associated with shorter progression-free survival. On a functional level, knockdown of kinesins in Ben-Men-1 cells and in the newly established anaplastic meningioma cell line NCH93 resulted in a significantly inhibited tumor cell proliferation upon siRNA-mediated downregulation of KIF11 in both cell lines by up to 95% and 71%, respectively. Taken together, in this study we were able to identify the prognostic and functional role of several kinesin family members of which KIF11 exhibits the most promising properties as a novel prognostic marker and therapeutic target, which may offer new treatment options for aggressive meningiomas.

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Section: Discussionmentioning

confidence: 99%

Identification of KIF11 As a Novel Target in Meningioma

Jungwirth

Tao

Moustafa

et al. 2019

Cancers

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“…KIF20A is a member of the kinesin family protein, which participates in spindle assembly during mitosis [19,20] . Numerous studies have shown that KIF20A promotes the proliferation, invasion and migration of cancer cells, and the high expression of KIF20A is significantly related to the occurrence, migration and prognosis of various tumors, such as glioma [21] , gastric cancer [22] , ovarian cancer [23] , and pancreatic cancer [24] . The prognostic value of KIF20A for HCC is also demonstrated [25] .…”

Section: Discussionmentioning

confidence: 99%

Identification and Analysis of the Key Genes associated with the Development from Liver Cirrhosis to Hepatocellular Carcinoma Based on Bioinformatics Analysis

Li¹,

Ding²,

Yan³

2020

Preprint

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Background: Hepatocellular carcinoma (HCC) is the most frequent primary liver tumor, and one of the most common malignant cancer with poor prognosis. Liver cirrhosis is the major risk factor for HCC. The aim of this study was to identify potential key genes associated with the development from liver cirrhosis to HCC and explore their potential mechanisms. Methods: Four microarray datasets GSE17548, GSE63898, GSE25097 and GSE89377 were downloaded from the Gene Expression Omnibus database. A protein-protein interaction (PPI) network was constructed using the STRING database, and potential hub genes were screened using MCODE plug-in in Cytoscape software. The Oncomine database was used to verify the expression of differential genes in cirrhosis and HCC. In order to further verify those hub genes, the hierarchical cluster between normal and HCC tissues was constructed using the UCSC Cancer Genomics Browser. The UALCAN database was used to verify the difference of hub genes in normal and HCC tissues and in different tumor grades. Finally, the cBioPortal online platform was used to analyze the association between the expression of hub genes and prognosis in HCC.Results: A total of 360 DEGs, including 280 downregulated and 80 upregulated genes, were identified. Gene ontology enrichment (GO) analysis showed that these DEGs were mainly enriched in monooxygenase activity, cofactor binding, and oxidoreductase activity (acting on CH-OH group of donors). The mainly enriched pathways were complement and coagulation cascades, prion diseases, and arachidonic acid metabolism. By extracting key modules from the PPI network, 16 hub genes were screened out. In the hierarchical cluster of hub genes between normal and HCC tissues, the results showed that the expression level of 16 hub genes in HCC tissues was significantly higher than that in normal tissues. In addition, expression level of the hub genes was significantly associated with the tumor grades. The survival analysis showed that six hub DEGs, including KIF20A,HMMR, RRM2, TPX2, TTK and UBE2C, were closely associated with the poor prognosis of HCC.Conclusion: Our study discovered six novel potential genes associated with the development from liver cirrhosis to HCC. These key genes may be used as prognostic biomarkers and molecular therapeutic targets for HCC.

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“…KIF20A is a member of the kinesin family protein which is located in the Golgi apparatus and participates in the dynamics of organelle and cell division [24, 25]. To date, KIF20A has been found to be highly expressed in various tumors such as cervical squamous cell carcinoma, ovarian clear-cell carcinoma, hepatocellular carcinoma, gastric cancer, bladder cancer, and glioma [10, 15, 16, 26–28]. With regard to that, further studies have confirmed that high expression of KIF20A is strongly associated with aggressive phenotypes of cancer.…”

Section: Discussionmentioning

confidence: 99%

“…It is an essential factor in the chromosome passenger complex (CPC) relocation; the latter is a key player in cytokinesis [9]. Until now, KIF20A is proved to be a key point in the development and progression of many cancers, such as leukemia, ovarian cancer, glioma, non-small cell lung cancer, bladder cancer, gastric cancer, and pancreatic cancer [10–16]. Recently, Li et al reported that KIF20A was obviously elevated in ovarian cancer promoting invasion and metastasis of the ovarian cancer cell line and conferring resistance to cisplatin [13].…”

Section: Introductionmentioning

confidence: 99%

Aberrant KIF20A Expression Is Associated with Adverse Clinical Outcome and Promotes Tumor Progression in Prostate Cancer

et al. 2019

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Purpose. KIF20A is essential in the process of spindle assembly and cytokinesis regulation. The role of KIF20A during tumorigenesis and tumor development has been well studied in several cancers. But the association between the KIF20A clinical role and prostate cancer (PCa) has not been reported yet. In this study, we investigated its potential prognostic effect and its role in progression of prostate cancer. Methods. Real-time quantitative polymerase chain reaction and Western blots were used to investigate the KIF20A transcription and translation levels in 7 pairs of fresh PCa tissue and adjacent normal prostate tissue. Immunohistochemistry (IHC) was used to investigate the KIF20A protein level in 114 PCa tissue samples. Bioinformatics analysis was performed to analyze the effect of KIF20A in oncologic prognosis in PCa patients. MTT assay, transwell assay, and colony formation assay in vitro and tumor formation assay in vivo were performed to evaluate the biological behavior of KIF20A in prostate cancer. Results. KIF20A was significantly elevated in tumor tissue compared with normal prostate tissue at both the mRNA and the protein level. High expression of KIF20A at the protein level was correlated with adverse clinicopathological features. Bioinformatics analysis showed that the high KIF20A expression group has a poor biochemical recurrence- (BCR-) free survival. Knocking down KIF20A suppressed the proliferation, migration, and invasion of the prostate cancer cell both in vitro and in vivo. Conclusions. Our data demonstrated that the high expression of KIF20A was associated with poor clinical outcome and targeting KIF20A could reduce proliferation, migration, and invasion of the prostate cancer cell, indicating that KIF20A might be a potential prognostic and therapeutic target for PCa patients.

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Upregulation of KIF20A correlates with poor prognosis in gastric cancer

Cited by 39 publications

References 39 publications

Identification of KIF11 As a Novel Target in Meningioma

Identification of KIF11 As a Novel Target in Meningioma

Identification and Analysis of the Key Genes associated with the Development from Liver Cirrhosis to Hepatocellular Carcinoma Based on Bioinformatics Analysis

Aberrant KIF20A Expression Is Associated with Adverse Clinical Outcome and Promotes Tumor Progression in Prostate Cancer

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