Aberrant KIF20A Expression Is Associated with Adverse Clinical Outcome and Promotes Tumor Progression in Prostate Cancer

Abstract

Purpose. KIF20A is essential in the process of spindle assembly and cytokinesis regulation. The role of KIF20A during tumorigenesis and tumor development has been well studied in several cancers. But the association between the KIF20A clinical role and prostate cancer (PCa) has not been reported yet. In this study, we investigated its potential prognostic effect and its role in progression of prostate cancer. Methods. Real-time quantitative polymerase chain reaction and Western blots were used to investigate the KIF20A transcription and translation levels in 7 pairs of fresh PCa tissue and adjacent normal prostate tissue. Immunohistochemistry (IHC) was used to investigate the KIF20A protein level in 114 PCa tissue samples. Bioinformatics analysis was performed to analyze the effect of KIF20A in oncologic prognosis in PCa patients. MTT assay, transwell assay, and colony formation assay in vitro and tumor formation assay in vivo were performed to evaluate the biological behavior of KIF20A in prostate cancer. Results. KIF20A was significantly elevated in tumor tissue compared with normal prostate tissue at both the mRNA and the protein level. High expression of KIF20A at the protein level was correlated with adverse clinicopathological features. Bioinformatics analysis showed that the high KIF20A expression group has a poor biochemical recurrence- (BCR-) free survival. Knocking down KIF20A suppressed the proliferation, migration, and invasion of the prostate cancer cell both in vitro and in vivo. Conclusions. Our data demonstrated that the high expression of KIF20A was associated with poor clinical outcome and targeting KIF20A could reduce proliferation, migration, and invasion of the prostate cancer cell, indicating that KIF20A might be a potential prognostic and therapeutic target for PCa patients.

Analysis of Hub Genes Involved in Distinction Between Aged and Fetal Bone Marrow Mesenchymal Stem Cells by Robust Rank Aggregation and Multiple Functional Annotation Methods

“…The mitotic kinesin, KIF20A, is essential for central spindle organization at anaphase as well as cytokinesis regulation (Zhang et al, 2019). It is supposedly a key factor in cell proliferation and invasion in many cancers (Sheng et al, 2018;Zhang et al, 2019). A recent study showed that KIF20A could reportedly be regulated by MYC (Pan et al, 2020).…”

FOXM1 modulates docetaxel resistance in prostate cancer by regulating KIF20A

“…Delaying resistance to docetaxel may improve the therapeutic outcome for PCa patients. Several studies indicate that development of docetaxel resistance is associated with FOXM1upregulation, and can be reversed by the down-regulation of FOXM1 expression in some cancers [12,[20][21]. High FOXM1 expression is also associated with the late stages of PCa, high gleason score, and poor prognosis [22].…”

FOXM1 modulates docetaxel resistance in prostate cancer by regulating KIF20A

“…Delaying resistance to docetaxel may improve the therapeutic outcome for PCa patients. Several studies indicate that development of docetaxel resistance is associated with FOXM1upregulation, and can be reversed by the down-regulation of FOXM1 expression in some cancers [12,[20][21]. High FOXM1 expression is also associated with late stages of PCa, high gleason score, and poor prognosis [22].…”