Upregulation of<i>MdMYB110a</i>is responsible for ABA-mediated colouration of type 2 red-fleshed apples

Sato, Hirosuke; Otagaki, Shungo; Y, Ono; Shiratake, Katsuhiro; Matsumoto, Shogo

doi:10.1080/14620316.2018.1452638

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…In many plant species, anthocyanin accumulation was mainly controlled by the transcriptional regulation of the R2R3 MYB TF. In apple (Malus domestica), MdMYB1 (An et al 2018), MdMYBA (Mahmoudi et al 2012), MdMYB10 (Espley et al 2007), and MYB110a (Sato et al 2019) were the crucial positive regulators of anthocyanin biosynthesis. MdMYB1 and MdMYBA were mainly involved in anthocyanin biosynthesis in the peel, MdMYB10 in the fruits and leaves, and MYB110a in the outer cortex of the fruit.…”

Section: Discussionmentioning

confidence: 99%

A NAC transcription factor, PpNAC1, regulates the expression of PpMYB10.1 to promote anthocyanin biosynthesis in the leaves of peach trees in autumn

Meng,

Sun,

et al. 2023

HORTIC. ADV.

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Red leaves in autumn are characteristic of a very early-maturing ripening variety of peach (Prunus persica). Analysis of the genetic factors and molecular mechanisms associated with the red-leaf phenotype can help breed peach very early maturing peach varieties. This study investigated the mechanisms underlying the red- and green-leaf phenotypes in autumn. Red compounds accumulated in the older but not younger leaves in the extremely early-maturing peach variety, ‘99-30-33’, and the leaves of the medium-maturing variety, ‘Zhongtao5 (CP5)’. Metabolic analysis showed that cyanidin-3-O-glucoside was the most abundant anthocyanin in the red leaves. The segregation of the progenies obtained from crossing revealed that the red leaves are a unique hereditary phenomenon not in line with Mendel’s law. BSA-seq and RNA-seq analyses suggest that PpNAC1 was essential for enhancing anthocyanin biosynthesis and was highly upregulated in red than in green leaves. Similar to PpNAC1, the anthocyanin activator, PpMYB10.1, was the only gene highly expressed in red leaves. Moreover, the functional genes involved in anthocyanin biosynthesis, such as Prunus persica Flavonoid 3'-hydroxylase (PpF3'H), Prunus persicaDihydroflavonol reductase (PpDFR), Prunus persica Leucoantho-cyanidin dioxygenase (Pp LDOX), Prunus persica Glutathione S-transferase (PpGST), and Prunus persica UDP-glucose, flavonoid-3-O-glucosyltransferase (PpUFGT), were upregulated in the older red leaves of 99-30-33 but downregulated in the younger 99-30-33 and green CP5 leaves. Yeast one-hybrid and dual-luciferase assays further confirmed that PpNAC1, which refers to 'Prunus persica NAC (NAM、A TAF1/2、CUC1/2)' bound to the promoter of PpMYB10.1, PpMYB10.1 stands for 'Prunus persica MYB (v-myb avian myeloblastosis viral) 10.1' and activated its expression along with those of PpGST and PpUFGT. These results provide insights into the mechanisms responsible for the development of red color in peach leaves in autumn.

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Section: Discussionmentioning

confidence: 99%

A NAC transcription factor, PpNAC1, regulates the expression of PpMYB10.1 to promote anthocyanin biosynthesis in the leaves of peach trees in autumn

Meng,

Sun,

et al. 2023

HORTIC. ADV.

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“…It was reported that UV-B irradiation enhanced the anthocyanin accumulation by upregulated MYBA in the fruit skin of 'Tsugaru' cultivar (Ban et al, 2007). Sato et al (2019) determined the correlation between anthocyanin accumulation and MYB110a expression in 'Nakano Shinku' apple; the expression level of MYB110a in the red part was significantly higher than in the white part. When darkgrown fruit was exposed to sunlight, the MYB1 transcript levels increased over several days, correlating with anthocyanin synthesis in the skin (Takos et al, 2006).…”

Section: Sunlight Stimulates the Coloration Of Apple Fruit By Modulating The Expression Of Transcription Factorsmentioning

confidence: 95%

Effect of Reflected Sunlight on Differential Expression of Anthocyanin Synthesis-Related Genes in Young Apple Fruit

Giap

Kim

Lee

et al. 2021

International Journal of Fruit Science

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Anthocyanin accumulation is responsible for the red color of the skin of apple fruits (Malus × domestica Borkh.). Environmental stimuli, such as light, temperature, soil factors, tree factors, and the application of chemicals can alter the synthesis of anthocyanins. Herein, we examined the expression of genes related to anthocyanin biosynthesis in the fruit of apple cultivars, 'Summer Prince' and 'Arisoo' at the early stage of fruit development 60 DAFB (days after full bloom). The expression of genes, which included structural and regulatory genes involved in anthocyanin biosynthesis, and light-responsive genes was determined in fruits developed under the effect of sunlight exposure for seven days. Apple fruits were divided in to: 'reflected sunlight', 'bagged', and 'control' groups. The expression levels of the anthocyanin synthesis-related genes were significantly different among the groups and between the shaded and reflected sides. We also determined the difference in coloration patterns in the different groups using the colorimetric coordinates method. The shaded side of apple fruits quickly turned more redder after exposure to reflected sunlight and the coloration was almost similar to that on the exposed side after the treatment. Strong correlation coefficients between anthocyanin-related gene expression levels and coloration patterns were obtained. This study shows that sunlight plays an important role in stimulating the coloration of apple fruit by regulating the expression of genes involved in anthocyanin accumulation during the early stage of fruit development. The present study assumes importance because redder fruits, generated through a non-transgenic approach would be more acceptable to consumers.

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“…In addition, the research explores consumers' purchasing intention, willingness to try and to buy RFA. The study aims at Several research has been carried out on the phytochemical composition of RFA, and their growing and post-harvest management factors [36,[78][79][80][81][82][83]. RFA varieties are of natural origin, and have a high content of phenolic compounds (flavonoids, flavanols, anthocyanins) which accounts for the red color of the fruit flesh, but also a bitter and astringent taste [78,84,85].…”

Section: Research Aimmentioning

confidence: 99%

Fruit Sector Strategic Management: An Exploration of Agro-food Chain Actors’ Perception of Market Sustainability of Apple Innovation

2020

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The fresh fruit industry is a highly dynamic sector in the food market. Fresh fruit chain actors have to adapt to the changing market environment in order to face upcoming challenges. The objective of this study is to analyze the market sustainability of red-fleshed apples (RFA) by exploring consumers’ and fresh fruit industry experts’ and stakeholders’ opinions in Italy and New Zealand. The study was carried out in Italy and New Zealand from December 2018 to June 2019 including 778 consumers and 29 expert and stakeholder interviews. Results show a promising market potential of RFA. Innovative attributes and nutritional value of RFA are the most important factors that push consumers’ interest and willingness to buy RFA. New Zealand consumers and women show a higher appreciation of RFA. The nutritional value of the fruit can be a strategic marketing management attribute especially for health-conscious consumers. However, experts think the market for RFA will develop slowly, and will remain a niche product. Some stakeholders are skeptical about consumer RFA appreciation, and fear that RFA are too radical a novelty on the market. The RFA may be perceived as not natural. There is the need to coordinate food chain stakeholders’ management practices on RFA. This allows to mitigate risks, set food standards acceptable for all chain stakeholders, and make sustainable economic investment on innovation.

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Upregulation ofMdMYB110ais responsible for ABA-mediated colouration of type 2 red-fleshed apples

Cited by 5 publications

References 35 publications

A NAC transcription factor, PpNAC1, regulates the expression of PpMYB10.1 to promote anthocyanin biosynthesis in the leaves of peach trees in autumn

A NAC transcription factor, PpNAC1, regulates the expression of PpMYB10.1 to promote anthocyanin biosynthesis in the leaves of peach trees in autumn

Effect of Reflected Sunlight on Differential Expression of Anthocyanin Synthesis-Related Genes in Young Apple Fruit

Fruit Sector Strategic Management: An Exploration of Agro-food Chain Actors’ Perception of Market Sustainability of Apple Innovation

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