Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix

Evangelista, Marta B.; Hsiong, Susan X.; Fernandes, Rui; Sampaio, Paula; Kong, Hyun Joon; Barrias, Cristina C.; Salema, R.; Barbosa, Mário A.; Mooney, David J.; Granja, Pedro L.

doi:10.1016/j.biomaterials.2007.04.028

Cited by 137 publications

(124 citation statements)

References 42 publications

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“…Their molecular weights, measured by TripleSEC as described in [22], were 1.5 × 10 5 Da (HMW) and 2.5 × 10 4 Da (LMW). The HMW alginate was partially oxidized with sodium periodate [29,30]. Aqueous carbodiimide chemistry was used to connect peptide N-terminal amino groups to alginate carboxyl groups via a peptide bond [31].…”

Section: Peptide-alginate Hydrogelsmentioning

confidence: 99%

“…The adequacy of this type of hydrogels for hMSCs entrapment has been previously demonstrated in vitro, namely in terms of their ability to preserve cell viability and support osteogenic differentiation [30,40,41], and also in vivo [22]. For this first set of studies, implants were performed at an ectopic site instead of a bone defect, to mitigate the effect of osteoinductive signals intrinsically present at the bone compartment, and to more specifically evaluate the direct impact of OGP analogs on hMSCs.…”

Section: In Vivo Studies With Hmscs-laden Ogp-alginate Hydrogelsmentioning

confidence: 99%

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Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Maia

Barbosa²,

Gomes

et al. 2014

Journal of Controlled Release

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The outcome of cell-based therapies can benefit from carefully designed cell carriers. A multifunctional injectable vehicle for the co-delivery of human mesenchymal stem cells (hMSCs) and osteoinductive peptides is proposed, to specifically direct hMSCs osteogenic differentiation. The osteogenic growth peptide (OGP) inspired the design of two peptides, where the bioactive portion of OGP was flanked by a protease-sensitive linker, or its scrambled sequence, to provide faster and slower release rates, respectively. Peptides were fully characterized and chemically grafted to alginate. Both OGP analogs released bioactive fragments in vitro, at different kinetics, which stimulated hMSCs proliferation and osteogenesis. hMSCs-laden OGP-alginate hydrogels were tested at an ectopic site in a xenograft mouse model. After 4 weeks, OGP-alginate hydrogels were more degraded and colonized by vascularized connective tissue than the control (without OGP). hMSCs were able to proliferate, migrate outward the hydrogels, produce endogenous extracellular matrix and mineralize it. Moreover, OGP-groups stimulated hMSCs osteogenesis, as compared with the control. Overall, the ability of the proposed platform to direct the fate of transplanted hMSCs in loco was demonstrated, and OGP-releasing hydrogels emerged as a potentially useful system to promote bone regeneration.

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Section: Peptide-alginate Hydrogelsmentioning

confidence: 99%

Section: In Vivo Studies With Hmscs-laden Ogp-alginate Hydrogelsmentioning

confidence: 99%

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Maia

Barbosa²,

Gomes

et al. 2014

Journal of Controlled Release

Self Cite

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show abstract

“…Earlier literature cites many applications of SA in agricultural applications after crosslinking with glutaraldehyde (GA) [13][14][15]. SA is one of the most versatile natural materials known to form hydrogels and films [16][17][18][19][20][21][22]. Drug-loaded films of SA are being used in pharmaceutical applications.…”

Section: Introductionmentioning

confidence: 99%

Sodium alginate/poly (ethylene oxide) blend hydrogel membranes for controlled release of valganciclovir hydrochloride

Mallikarjuna

Rao

Siraj

et al. 2012

Designed Monomers and Polymers

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Sodium alginate (SA) and poly (ethylene oxide) PEO blend membranes were prepared by solvent casting method for the controlled release of valganciclovir hydrochloride, an antiHIV drug. The prepared membranes were thin, flexible, smooth, and characterized by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), X-ray diffraction (X-RD), scanning electron microscopy (SEM), and tensile strength measurement. FTIR was used to understand the formation of hydrogen bonding between SA and PEO. The DSC and X-RD studies were performed to understand the crystalline nature of drug after encapsulation into the membranes. SEM was used to study the surface morphology of the membranes. Tensile strength measurements revealed the mechanical strength of the membranes. In vitro release studies indicated a dependence of release rate on the extent of crosslinking, amount of drug loading, and the amount of PEO, but slow release rates was extended up to 12 h. Cumulative release data were fitted onto to an empirical equation to compute diffusional exponent (n), which indicated the nonFickian trend for drug release.

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“…47,48 Another study developed RGD-modified alginate microbeads of 1 mm in diameter, but bead degradation and cell release were not mentioned. 49 A recent study fabricated alginate microbeads of approximately 200 mm for cell encapsulation; no microbead degradation or cell release was reported. 10 Therefore, compared to these previous studies, the uniqueness of the present study was that hUCMSCs were encapsulated in alginate-fibrin microbeads with several hundred mm in size suitable for injection, and the microbeads were then packed into a hydrogel matrix for muscle tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Fast-Degradable Microbeads Encapsulating Human Umbilical Cord Stem Cells in Alginate for Muscle Tissue Engineering

Liu

Zhou²,

Weir³

et al. 2012

Tissue Engineering Part A

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Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix

Cited by 137 publications

References 42 publications

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

Sodium alginate/poly (ethylene oxide) blend hydrogel membranes for controlled release of valganciclovir hydrochloride

Fast-Degradable Microbeads Encapsulating Human Umbilical Cord Stem Cells in Alginate for Muscle Tissue Engineering

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